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Difference between revisions of "High signal at zero oxygen"

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{{MitoPedia without banner
{{MitoPedia without banner
|description='A ''high signal at zero oxygen''' may be observed during [[zero calibration]]. The following instructions show how to further localize and possibly solve this technical problem.
|abbr=''R''0
|description=A '''high signal at zero oxygen''' may be observed during [[zero calibration]]. The following instructions show how to further localize and possibly solve this technical problem.
|info= [[Zero_calibration | Zero calibration]], [[OroboPOS-Connector_MitoPedia#Cleaning_the_electrical_connections | OroboPOS-Connector]]
}}
}}
== High zero current ==
== High zero current test==
:::: In order to localize the problem, the following test should be performed:
::::1. Unscrew the POS from the POS-Connector and unplug the POS-Connector from the O2k-main unit.
::::2. Switch on the O2k and start DatLab.
::::3. Record the signal for 10 min.
::::4. Connect the POS-Connector to the O2k-main unit without the POS. Set an event in DatLab called "POS-Connector".
::::5. Record the signal for 10 min.
::::6. Remove the membrane from the POS and wash the cathode and anode with distilled water.
::::7. Screw the POS to the POS-Connector. Set an event in DatLab called "POS".
::::8. Record the signal for 10 min.
::::9. Send us the file.
 


:::[[File:High zero current test 2.png|1000px]]
::::*Note: Before sending us the O2k or any spare components, please contact us via our O2k-Open Support system: [[O2k-Open Support]]
:::::References:  
:::::References:  
::::::[1] [https://wiki.oroboros.at/index.php/OroboPOS-Connector_MitoPedia#Cleaning_the_electrical_connections Cleaning the electrical connections]
::::::[1] [https://wiki.oroboros.at/index.php/OroboPOS-Connector_MitoPedia#Cleaning_the_electrical_connections Cleaning the electrical connections]
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::::::[4] [[MiPNet14.06 Instrumental O2 background]]
::::::[4] [[MiPNet14.06 Instrumental O2 background]]


::: '''Example:'''
::: '''Example:''' First, the OroboPOS-Connector and the POS were disassembled and disconnected from the main unit. Second, the OroboPOS-Connector was connected to the O2k-Main unit without the OroboPOS. Third, the OroboPOS was connected to the OroboPOS-Connector without membranes. After each step, the O<sub>2</sub> raw signal [V] was recorded for 10 min. The marks were set to the O<sub>2</sub> raw signal [V] to evaluate the zero current.
::::[[File:High zero current good example.png|600px]]
::::[[File:High zero current good example.png|700px]]
::::[[File:High zero current test bad exmaple.png|600px]]
::::'''Figure 1.''' High zero current test.  Upper panel: Zero current is in the range of ±0.00099 V. Lower panel: Zero current is higher than 0.00099 V when the OroboPOS-Connector is connected to the O2k-Main unit and when the OroboPOS is connected to the OroboPOS-Connector, which means that both the OroboPOS-Connector and OroboPOS have electrical problems. First, the OroboPOS-Connector and the POS were disassembled and disconnected from the main unit. Second, the OroboPOS-Connector was connected to the O2k-Main unit without the OroboPOS. Third, the OroboPOS was connected to the OroboPOS-Connector without membranes. After each step, the O<sub>2</sub> raw signal [V] was recorded for 10 min. The marks were set to the O<sub>2</sub> raw signal [V] to evaluate the zero current.


::::[[File:High zero current test bad exmaple.png|700px]]
::::'''Figure 1.''' High zero current test.  '''Upper panel:''' Zero current is in the range of ±0.00099 V. '''Lower panel:''' Zero current is higher than 0.00099 V when the OroboPOS-Connector is connected to the O2k-Main unit and when the OroboPOS is connected to the OroboPOS-Connector, indicating a potential electrical problem with both the OroboPOS-Connector and the OroboPOS.


== Keywords ==
== Keywords ==

Revision as of 10:53, 8 April 2021


                  


O2k-Open Support

High signal at zero oxygen



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MitoPedia O2k and high-resolution respirometry: O2k-Open Support 



Description

A high signal at zero oxygen may be observed during zero calibration. The following instructions show how to further localize and possibly solve this technical problem.

Abbreviation: R0

Reference: Zero calibration, OroboPOS-Connector

High zero current test

1. Unscrew the POS from the POS-Connector and unplug the POS-Connector from the O2k-main unit.
2. Switch on the O2k and start DatLab.
3. Record the signal for 10 min.
4. Connect the POS-Connector to the O2k-main unit without the POS. Set an event in DatLab called "POS-Connector".
5. Record the signal for 10 min.
6. Remove the membrane from the POS and wash the cathode and anode with distilled water.
7. Screw the POS to the POS-Connector. Set an event in DatLab called "POS".
8. Record the signal for 10 min.
9. Send us the file.


References:
[1] Cleaning the electrical connections
[2] MiPNet19.18B POS-service
[3] MiPNet06.03 POS-calibration-SOP
[4] MiPNet14.06 Instrumental O2 background
Example: First, the OroboPOS-Connector and the POS were disassembled and disconnected from the main unit. Second, the OroboPOS-Connector was connected to the O2k-Main unit without the OroboPOS. Third, the OroboPOS was connected to the OroboPOS-Connector without membranes. After each step, the O2 raw signal [V] was recorded for 10 min. The marks were set to the O2 raw signal [V] to evaluate the zero current.
High zero current good example.png
High zero current test bad exmaple.png
Figure 1. High zero current test. Upper panel: Zero current is in the range of ±0.00099 V. Lower panel: Zero current is higher than 0.00099 V when the OroboPOS-Connector is connected to the O2k-Main unit and when the OroboPOS is connected to the OroboPOS-Connector, indicating a potential electrical problem with both the OroboPOS-Connector and the OroboPOS.

Keywords


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