Difference between revisions of "High signal at zero oxygen"
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{{MitoPedia without banner | {{MitoPedia without banner | ||
|abbr=''R''0 | |abbr=''R''0 | ||
|description=' | |description=A '''high signal at zero oxygen''' may be observed during [[zero calibration]]. The following instructions show how to further localize and possibly solve this technical problem. | ||
|info= [[Zero_calibration | Zero calibration]], [[OroboPOS-Connector_MitoPedia#Cleaning_the_electrical_connections | OroboPOS-Connector]] | |info= [[Zero_calibration | Zero calibration]], [[OroboPOS-Connector_MitoPedia#Cleaning_the_electrical_connections | OroboPOS-Connector]] | ||
}} | }} | ||
== High zero current == | == High zero current test== | ||
::::1. Unscrew the POS from the POS-Connector and unplug the POS-Connector from the O2k-main unit. | |||
::::2. Switch on the O2k and start DatLab. | |||
::::3. Record the signal for 10 min. | |||
::::4. Connect the POS-Connector to the O2k-main unit without the POS. Set an event in DatLab called "POS-Connector". | |||
::::5. Record the signal for 10 min. | |||
::::6. Remove the membrane from the POS and wash the cathode and anode with distilled water. | |||
::::7. Screw the POS to the POS-Connector. Set an event in DatLab called "POS". | |||
::::8. Record the signal for 10 min. | |||
::::9. Send us the file. | |||
:::::References: | :::::References: | ||
::::::[1] [https://wiki.oroboros.at/index.php/OroboPOS-Connector_MitoPedia#Cleaning_the_electrical_connections Cleaning the electrical connections] | ::::::[1] [https://wiki.oroboros.at/index.php/OroboPOS-Connector_MitoPedia#Cleaning_the_electrical_connections Cleaning the electrical connections] | ||
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::: '''Example:''' First, the OroboPOS-Connector and the POS were disassembled and disconnected from the main unit. Second, the OroboPOS-Connector was connected to the O2k-Main unit without the OroboPOS. Third, the OroboPOS was connected to the OroboPOS-Connector without membranes. After each step, the O<sub>2</sub> raw signal [V] was recorded for 10 min. The marks were set to the O<sub>2</sub> raw signal [V] to evaluate the zero current. | ::: '''Example:''' First, the OroboPOS-Connector and the POS were disassembled and disconnected from the main unit. Second, the OroboPOS-Connector was connected to the O2k-Main unit without the OroboPOS. Third, the OroboPOS was connected to the OroboPOS-Connector without membranes. After each step, the O<sub>2</sub> raw signal [V] was recorded for 10 min. The marks were set to the O<sub>2</sub> raw signal [V] to evaluate the zero current. | ||
::::[[File:High zero current good example.png| | ::::[[File:High zero current good example.png|700px]] | ||
::::[[File:High zero current test bad exmaple.png|700px]] | |||
::::'''Figure 1.''' High zero current test. '''Upper panel:''' Zero current is in the range of ±0.00099 V. '''Lower panel:''' Zero current is higher than 0.00099 V when the OroboPOS-Connector is connected to the O2k-Main unit and when the OroboPOS is connected to the OroboPOS-Connector, indicating a potential electrical problem with both the OroboPOS-Connector and the OroboPOS. | |||
== Keywords == | == Keywords == |
Revision as of 11:53, 8 April 2021
High signal at zero oxygen |
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
Description
A high signal at zero oxygen may be observed during zero calibration. The following instructions show how to further localize and possibly solve this technical problem.
Abbreviation: R0
Reference: Zero calibration, OroboPOS-Connector
High zero current test
- 1. Unscrew the POS from the POS-Connector and unplug the POS-Connector from the O2k-main unit.
- 2. Switch on the O2k and start DatLab.
- 3. Record the signal for 10 min.
- 4. Connect the POS-Connector to the O2k-main unit without the POS. Set an event in DatLab called "POS-Connector".
- 5. Record the signal for 10 min.
- 6. Remove the membrane from the POS and wash the cathode and anode with distilled water.
- 7. Screw the POS to the POS-Connector. Set an event in DatLab called "POS".
- 8. Record the signal for 10 min.
- 9. Send us the file.
- Example: First, the OroboPOS-Connector and the POS were disassembled and disconnected from the main unit. Second, the OroboPOS-Connector was connected to the O2k-Main unit without the OroboPOS. Third, the OroboPOS was connected to the OroboPOS-Connector without membranes. After each step, the O2 raw signal [V] was recorded for 10 min. The marks were set to the O2 raw signal [V] to evaluate the zero current.
- Figure 1. High zero current test. Upper panel: Zero current is in the range of ±0.00099 V. Lower panel: Zero current is higher than 0.00099 V when the OroboPOS-Connector is connected to the O2k-Main unit and when the OroboPOS is connected to the OroboPOS-Connector, indicating a potential electrical problem with both the OroboPOS-Connector and the OroboPOS.
Keywords
- Bioblast links: Oxygen signal - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Specific
- O2k-Procedures
- MiPNet O2k-Procedures
- Other keyword lists
MitoPedia O2k and high-resolution respirometry: O2k hardware, O2k-Open Support, O2k-Respirometry