Difference between revisions of "Horvath 2012 Abstract Bioblast"
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|abstract=[[File:Gergö.jpg|right|150px|Gergö Horvath]] | |abstract=[[File:Gergö.jpg|right|150px|Gergö Horvath]] | ||
The mitochondria play a key role in the pathogenesis of different diseases including the illnesses of the heart. According to the World Health Organization, chronic diseases are responsible for 63% of all deaths in the world, with cardiovascular disease as the leading cause of death. The new achievements of the cardiac muscle mitochondria functions can be further used to develope new therapeutic strategies. | The mitochondria play a key role in the pathogenesis of different diseases including the illnesses of the heart. According to the World Health Organization, chronic diseases are responsible for 63% of all deaths in the world, with cardiovascular disease as the leading cause of death. The new achievements of the cardiac muscle mitochondria functions can be further used to develope new therapeutic strategies. | ||
The most important parameters of the heart mitochondrial activity are the oxidative phosphorylation capacity and the mitochondrial membrane potential with these two properties of the mitochondria we can determinate coupling state of the respiration and the impact of the mitochondrial substrate on the membrane potential.[1] The O2k-Fluorometry combines the optical measurement with high-resolution respirometry and with this new technology we can detect the changes of both parameters online. | The most important parameters of the heart mitochondrial activity are the oxidative phosphorylation capacity and the mitochondrial membrane potential with these two properties of the mitochondria we can determinate coupling state of the respiration and the impact of the mitochondrial substrate on the membrane potential.[1] The O2k-Fluorometry combines the optical measurement with high-resolution respirometry and with this new technology we can detect the changes of both parameters online. | ||
The experiments we carried out on mouse heart homogenate. The measurements were performed on Oxygraph-2k (Oroboros Instruments, Innsbruck). We detect the oxygen-consumption with Clack-electrode and the membrane potential changes with safranin fluorescence dye on 480 nm. | The experiments we carried out on mouse heart homogenate. The measurements were performed on Oxygraph-2k (Oroboros Instruments, Innsbruck). We detect the oxygen-consumption with Clack-electrode and the membrane potential changes with safranin fluorescence dye on 480 nm. | ||
The experiments we measured with the SUIT protocol, with little modification[2]. We investigated the complex I, II, I+II in different coupling control levels. | The experiments we measured with the SUIT protocol, with little modification[2]. We investigated the complex I, II, I+II in different coupling control levels. | ||
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[2]Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58. | [2]Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58. | ||
|keywords=Mitochondrial membrane potential, cardiac muscle | |keywords=Mitochondrial membrane potential, cardiac muscle | ||
|journal=Mitochondr Physiol Network | |journal=Mitochondr Physiol Network | ||
Revision as of 17:02, 27 November 2012
| Horvath G (2012) Respiratory substrates effects on mitochondrial membrane potential, experiments with mouse cardiac muscle homogenate, Mitochondr Physiol Network 17.12. |
Link: MiPNet17.12 Bioblast 2012 - Open Access
Horvath G, Eigentler A, Gnaiger E, Tretter L (2012)
Event: Bioblast 2012
The mitochondria play a key role in the pathogenesis of different diseases including the illnesses of the heart. According to the World Health Organization, chronic diseases are responsible for 63% of all deaths in the world, with cardiovascular disease as the leading cause of death. The new achievements of the cardiac muscle mitochondria functions can be further used to develope new therapeutic strategies.
The most important parameters of the heart mitochondrial activity are the oxidative phosphorylation capacity and the mitochondrial membrane potential with these two properties of the mitochondria we can determinate coupling state of the respiration and the impact of the mitochondrial substrate on the membrane potential.[1] The O2k-Fluorometry combines the optical measurement with high-resolution respirometry and with this new technology we can detect the changes of both parameters online.
The experiments we carried out on mouse heart homogenate. The measurements were performed on Oxygraph-2k (Oroboros Instruments, Innsbruck). We detect the oxygen-consumption with Clack-electrode and the membrane potential changes with safranin fluorescence dye on 480 nm. The experiments we measured with the SUIT protocol, with little modification[2]. We investigated the complex I, II, I+II in different coupling control levels.
Reference:
[1]Assessing mitochondrial dysfunction in cells Brand MD, Nicholls DG Biochem. J. (2011) 435, 297–312
[2]Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58.
• Keywords: Mitochondrial membrane potential, cardiac muscle
Labels:
Organism: Mouse
Tissue;cell: Cardiac muscle"Cardiac muscle" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property.
Preparation: Homogenate
Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k, Spectrofluorimetry"Spectrofluorimetry" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
Affiliations and author contributions
Horvath Gergoe(1), Eigentler Andrea(2), Gnaiger Erich(2,3), Tretter Laszlo(1)
1 Department of Medical Biochemistry, Semmelweis University, Budapest, Hungary
2 Medical University of Innsbruck, Department of Visceral, Transplant and Thoracic Surgery, D. Swarovski Research Laboratory, Innsbruck, Austria
3 Oroboros Instruments Corp, High Resolution Respirometry, Innsbruck, Austria Email: [email protected]
