Hroudova 2012 European Psychiatry

Hroudova J, Fisar Z, Korabecny J, Kuca K, Jirak R, Raboch J (2012) P-451 - changes of cellular respiration in patients with Alzheimer's disease. European Psychiatry 27: 01

» doi.org/10.1016/S0924-9338(12)74618-7

Hroudova J, Fisar Z, Korabecny J, Kuca K, Jirak R, Raboch J (2012) European Psychiatry

Abstract: Introduction: Alzheimer's disease (AD) is the most frequent neurodegenerative disease, characterized by progressive decline in variety of higher brain functions - memory, orientation, and thinking. According to increasing evidences, mitochondrial insufficiencies contribute to pathology of AD; changes were described in AD brains, blood cells and human fibroblasts.

Objectives: On molecular level, oxygen and glucose metabolism is altered and energy metabolism is impaired. Mitochondrial abnormalities and alterations in mitochondrial enzymes, especially complex I and cytochrome c oxidase, were observed. However, the cause and important aspects of AD mechanism have not yet been sufficiently clarified.

Aims: The aim of our study was to find whether kinetics of oxygen consumption is modified in AD patients. Further, we afford to suggest parameters that could be suitable as AD markers.

Methods: AD patients and healthy control group were included in the study. Respiratory rate of mitochondria, as measure of total activity of the system of oxidative phosphorylation (OXPHOS), was measured in mitochondria using oxygraph with Clark-type electrodes. High resolution respirometry was performed in intact as well as in permeabilized platelets.

Results: Our results indicate significantly lower respiratory rate in intact platelets as well as lower respiratory capacity of electron transfer system in patients with AD compared to controls.

Conclusions: We propose that decrease in oxygen consumption may participate in pathophysiology of AD, and respiratory rate in platelets could be AD marker. • Keywords: Alzheimer's disease, human platelets, disease marker, neurodegeneration, mitochondrial insufficiencies, oxygen and glucose metabolism

• O2k-Network Lab: CZ Prague Fisar Z

Labels: Pathology: Alzheimer's disease"Alzheimer's disease" is not in the list (Aging;senescence, Alzheimer's, Autism, Cancer, Cardiovascular, COPD, Diabetes, Inherited, Infectious, Myopathy, ...) of allowed values for the "Diseases" property.  Stress:Mitochondrial Disease; Degenerative Disease and Defect"Mitochondrial Disease; Degenerative Disease and Defect" is not in the list (Cell death, Cryopreservation, Ischemia-reperfusion, Permeability transition, Oxidative stress;RONS, Temperature, Hypoxia, Mitochondrial disease) of allowed values for the "Stress" property.  Organism: Human  Tissue;cell: Blood Cell; Suspension Culture"Blood Cell; Suspension Culture" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property.  Preparation: Intact Cell; Cultured; Primary"Intact Cell; Cultured; Primary" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property., Permeabilized cells  Enzyme: Complex I, Complex IV; Cytochrome c Oxidase"Complex IV; Cytochrome c Oxidase" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property. 

Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property. 

HRR: Oxygraph-2k