Jorgensen 2009 Am J Physiol Endocrinol Metab

Jørgensen W, Gam C, Andersen JL, Schjerling P, Scheibye-Knudsen M, Mortensen OH, Grunnet N, Nielsen MO, Quistorff B (2009) Changed mitochondrial function by pre- and/or postpartum diet alterations in sheep. Am J Physiol Endocrinol Metab 297: E1349-E1357.

» PMID: 19826104 Open Access

Joergensen W, Gam C, Andersen JL, Schjerling P, Scheibye-Knudsen M, Mortensen OH, Grunnet N, Nielsen MO, Quistorff B (2009) Am J Physiol Endocrinol Metab

Abstract: In a sheep model, we investigated diet effects on skeletal muscle mitochondria to look for fetal programming. During pregnancy, ewes were fed normally (N) or were 50% food restricted (L) during the last trimester, and lambs born to these ewes received a normal (N) or a high-fat diet (H) for the first 6 mo of life. We examined mitochondrial function in permeabilized muscle fibers from the lambs at 6 mo of age (adolescence) and after 24 mo of age (adulthood). The postpartum H diet for the lambs induced an ~30% increase (P < 0.05) of mitochondrial V_O2max and an ~50% increase (P < 0.05) of the respiratory coupling ratio (RCR) combined with lower levels of UCP3 and PGC-1αmRNA levels (P < 0.05). These effects proved to be reversible by a normal diet from 6 to 24 mo of age. However, at 24 mo, a long-term effect of the maternal gestational diet restriction (fetal programming) became evident as a lower VO2max (~40%, P < 0.05), a lower state 4 respiration (~40%, P < 0.05), and lower RCR (~15%, P < 0.05). Both PGC-1α and UCP3 mRNA levels were increased (P < 0.05). Two analyzed muscles were affected differently, and muscle rich in type I fibers was more susceptible to fetal programming. We conclude that fetal programming, seen as a reduced V_O2max in adulthood, results from gestational undernutrition. Postnatal high-fat diet results in a pronounced RCR and V_O2max increase in adolescence. However, these effects are reversible by diet correction and are not maintained in adulthood. • Keywords: Metabolic syndrome, high-fat diet, nutrient restriction, maternal diet, respiratory coupling control ratio

• O2k-Network Lab: DK Copenhagen Quistorff B

Labels:

Organism: Mammals"Mammals" is not in the list (Human, Pig, Mouse, Rat, Guinea pig, Bovines, Horse, Dog, Rabbit, Cat, ...) of allowed values for the "Mammal and model" property.  Tissue;cell: Skeletal muscle  Preparation: Permeabilized tissue 

Coupling state: OXPHOS 

HRR: Oxygraph-2k