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Difference between revisions of "Kemp 2000 Thermochim Acta (Vol 349)"

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|injuries=Genetic Defect; Knockdown; Overexpression
|instruments=Oxygraph-2k
|organism=Other Mammal
|organism=Other Mammal
|topics=Respiration; OXPHOS; ETS Capacity, Substrate; Glucose; TCA Cycle
|preparations=Intact Cell; Cultured; Primary
|instruments=Oxygraph-2k, Method
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Revision as of 12:23, 6 September 2011

Publications in the MiPMap
Kemp RB, Guan YH (2000) The application of heat flux measurements to improve the growth of mammalian cells in culture. Thermochim. Acta 349: 23-30.


Kemp RB, Guan YH (2000) Thermochim. Acta

Abstract: There is a paucity of on-line biosensors to monitor the changes in the processes that occur in cultures of animal cells growing in a bioreactor. It would be preferable for such a probe to measure a metabolic variable. Since there is a sound theoretical basis for supposing that heat flux is a reflection of the overall metabolic flux, it was decided to explore the possibilities of marrying heat conduction calorimetry that measures heat flow rate with a dielectric spectrometer that estimates the viable biomass. The ratio of the two signals gives scalar heat flux. Previous work had shown that this variable could be used to validate the growth reaction of the cells in terms of the Mayer enthalpy balance method. It was revealed by the description of this reaction that the ratio of glucose to glutamine in the culture medium was incorrect. The finding stimulated the formulation of a revised medium that improved the specific growth rate of CHO320 cells producing recombinant interferon-Ξ³ (IFN-Ξ³), as well as allowed a considerably greater yield of the target protein. In addition, the glucose and lactate fluxes decreased, indicating that there was less demand for biosynthetic precursors from glucose. β€’ Keywords: Heat flux, Genetically engineered cells, Continuous culture, Fed-batch culture, Medium optimisation


Labels:


Organism: Other Mammal"Other Mammal" is not in the list (Human, Pig, Mouse, Rat, Guinea pig, Bovines, Horse, Dog, Rabbit, Cat, ...) of allowed values for the "Mammal and model" property. 

Preparation: Intact Cell; Cultured; Primary"Intact Cell; Cultured; Primary" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property. 



HRR: Oxygraph-2k