Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Kidane 1997 J Thermal Anal

From Bioblast
Publications in the MiPMap
Kidane AH, Guan Y, Evans PM, Kaderbhai MA, Kemp RB (1997) Comparison of heat flux in wild-type and genetically-engineered Chinese hamster ovary cells. J Thermal Anal 49: 771-783.

Β» doi10.1007/BF01996760

Kidane AH, Guan Y, Evans PM, Kaderbhai MA, Kemp RB (1997) J Thermal Anal

Abstract: It is claimed, though not without dispute, that genetically engineered mammalian cells grow more slowly than their progenitor cells because the recombinant gene system causes a metabolic burden. This was found to be the case for CHO cells transfected with expression vectors forcytochrome b5. The slower growth was associated with lower metabolic activity measured by heat flux and mitochondrial activity (rhodamine 123 fluorescence). The calorimetric-respirometric ratio was similar for all cell types, implying that the greater fluxes of glucose and glutamine in the recombinant cells was channelled to biosynthesis. This demand probably restricted the supply of pyruvate to the mitochondria in these cells. β€’ Keywords: Cytochrome b 5, Genetically-engineered cells, Heat flux, Metabolic burden, Mitochondrial activity, hamster


Labels:

Stress:Genetic Defect; Knockdown; Overexpression"Genetic Defect; Knockdown; Overexpression" is not in the list (Cell death, Cryopreservation, Ischemia-reperfusion, Permeability transition, Oxidative stress;RONS, Temperature, Hypoxia, Mitochondrial disease) of allowed values for the "Stress" property.  Organism: Mammals"Mammals" is not in the list (Human, Pig, Mouse, Rat, Guinea pig, Bovines, Horse, Dog, Rabbit, Cat, ...) of allowed values for the "Mammal and model" property. 

Preparation: Intact Cell; Cultured; Primary"Intact Cell; Cultured; Primary" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property. 



HRR: Oxygraph-2k