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== Abstract continued ==
ROUTINE control ratio was significantly decreased (''p''=0.035) in 4 hours ApoA1 treated intact cells. This indicates that ROUTINE respiration is operating closer to ET capacity in control cells than in ApoA1 cells. Respiration complex IV proteins were significantly decreased in ApoA1 treated cells (''p''=0.018). Complex specific respiration in permeabilized cells was not significantly affected by ApoA1 treatment. First hypothesis was confirmed by results obtained with intact cells. These results indicate ApoA1 increased ET capacity in leukocytes. Increased ET capacity leads to increased ROUTINE respiration and residual respiration. Second hypothesis was confirmed since significant decrease in CIV protein was discovered with western blot. Based on this study, it can be concluded that ApoA1 increases cell respiration in intact leukocytes and affects respiration complex proteins. However, this study was limited preliminary study and further experiments with larger sample size are needed to confirm the obtained results. Due to several artifacts significant results were not obtained regarding complex specific respiration in permeabilized cells. Respiration in permeabilized cells should be examined in future studies to gain knowledge of more detailed effects of ApoA1 to cell respiration. Nonetheless the results obtained here are promising for the future use of leukocytes to reflect metabolism in muscle cells.
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