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Linearity

From Bioblast
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high-resolution terminology - matching measurements at high-resolution


Linearity

Description

Linearity is the ability of the method to produce test results that are proportional, either directly or by a well-defined mathematical transformation, to the concentration of the analyte in samples within a given range. This property is inherent in the Beer-Lambert law for absorbance alone, but deviations occur in scattering media. It is also a property of fluorescence, but a fluorophore may not exhibit linearity, particularly over a large range of concentrations.


Reference: Paul WL (1991) USP perspectives on analytical methods evaluation. Pharm. Technol. 15: 130-141. Elwell CE (1995) A practical users guide to near infrared spectroscopy. Hamamatsu Photonics, 155pp.



MitoPedia methods: Respirometry, Fluorometry, Spectrophotometry 


MitoPedia O2k and high-resolution respirometry: Oroboros QM 



Contributed by Harrison DK, 2011-11-18