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Meszaros 2018 Mt Med Hinxton - Revision history
2024-03-29T01:59:49Z
Revision history for this page on the wiki
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https://wiki.oroboros.at/index.php?title=Meszaros_2018_Mt_Med_Hinxton&diff=223745&oldid=prev
Gnaiger Erich at 17:03, 10 January 2022
2022-01-10T17:03:59Z
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 17:03, 10 January 2022</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Mitochondrial ''p<sub>50</sub>'' measurement is a quick, highly accurate and sensitive way for early detection of reduced CIV excess capacity, as affinity of mitochondria for O<sub>2</sub> decreases already at a slight CIV inhibition. Determination of mitochondrial O<sub>2</sub> dependence has implications in a wide range of biomedical research topics, particularly in studies with CIV mutations (e.g. Leigh syndrome), or with small inhibitory molecules (nitric oxide). ''O2'''kinetics''''' provides a fast and simple method for the detection of CIV impairment and extends standard OXPHOS analysis to the intracellular O<sub>2</sub> regime ''in vivo'', reflecting low physiological O<sub>2</sub> concentrations or tissue hypoxia.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Mitochondrial ''p<sub>50</sub>'' measurement is a quick, highly accurate and sensitive way for early detection of reduced CIV excess capacity, as affinity of mitochondria for O<sub>2</sub> decreases already at a slight CIV inhibition. Determination of mitochondrial O<sub>2</sub> dependence has implications in a wide range of biomedical research topics, particularly in studies with CIV mutations (e.g. Leigh syndrome), or with small inhibitory molecules (nitric oxide). ''O2'''kinetics''''' provides a fast and simple method for the detection of CIV impairment and extends standard OXPHOS analysis to the intracellular O<sub>2</sub> regime ''in vivo'', reflecting low physiological O<sub>2</sub> concentrations or tissue hypoxia.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|editor=[[Kandolf G]], [[Meszaros AT]],</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|editor=[[Kandolf G]], [[Meszaros AT]],</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|mipnetlab=<del style="font-weight: bold; text-decoration: none;">AT Innsbruck Gnaiger E, </del>AT Innsbruck Oroboros</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|mipnetlab=AT Innsbruck Oroboros</div></td></tr>
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<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">== Affiliations ==</ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">::::Meszaros AT(1), Haider M(2), Di Marcello M(1,3), Gnaiger E(1,3)</ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">:::::#Oroboros Instruments, Innsbruck, Austria</ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">:::::#Steinhauser & Haider Technology Consulting OG, Innsbruck, Austria</ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">:::::#Daniel Swarovski Research Lab, Dept Visceral, Transplant Thoracic Surgery, Medical Univ Innsbruck, Austria. - andras.meszaros@oroboros.at</ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">== Cited by ==</ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">{{Template:Cited by Huete-Ortega M 2021 MitoFit Dark respiration}}</ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>{{Labeling</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>{{Labeling</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|area=Respiration, mt-Medicine</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|area=Respiration, mt-Medicine</div></td></tr>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>}}</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>}}</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">== Affiliations ==</del></div></td><td colspan="2"></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">::::Meszaros AT(1), Haider M(2), Di Marcello M(1,3), Gnaiger E(1,3)</del></div></td><td colspan="2"></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">:::::#Oroboros Instruments, Innsbruck, Austria</del></div></td><td colspan="2"></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">:::::#Steinhauser & Haider Technology Consulting OG, Innsbruck, Austria</del></div></td><td colspan="2"></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">:::::#Daniel Swarovski Research Lab, Dept Visceral, Transplant Thoracic Surgery, Medical Univ Innsbruck, Austria. - andras.meszaros@oroboros.at</del></div></td><td colspan="2"></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">== Cited by ==</del></div></td><td colspan="2"></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">{{Template:Cited by Huete-Ortega M 2021 MitoFit Dark respiration}}</del></div></td><td colspan="2"></td></tr>
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Gnaiger Erich
https://wiki.oroboros.at/index.php?title=Meszaros_2018_Mt_Med_Hinxton&diff=214882&oldid=prev
Huete-Ortega Maria at 13:49, 1 February 2021
2021-02-01T13:49:15Z
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 13:49, 1 February 2021</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|pathways=N, CIV, NS</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|pathways=N, CIV, NS</div></td></tr>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Affiliations ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Affiliations ==</div></td></tr>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::::#Steinhauser & Haider Technology Consulting OG, Innsbruck, Austria</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::::#Steinhauser & Haider Technology Consulting OG, Innsbruck, Austria</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::::#Daniel Swarovski Research Lab, Dept Visceral, Transplant Thoracic Surgery, Medical Univ Innsbruck, Austria. - andras.meszaros@oroboros.at</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::::#Daniel Swarovski Research Lab, Dept Visceral, Transplant Thoracic Surgery, Medical Univ Innsbruck, Austria. - andras.meszaros@oroboros.at</div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">== Cited by ==</ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">{{Template:Cited by Huete-Ortega M 2021 MitoFit Dark respiration}}</ins></div></td></tr>
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Huete-Ortega Maria
https://wiki.oroboros.at/index.php?title=Meszaros_2018_Mt_Med_Hinxton&diff=180577&oldid=prev
Gnaiger Erich: Gnaiger Erich moved page Meszaros 2018 Mitochondrial Medicine 2018 Hinxton UK to Meszaros 2018 Mt Med Hinxton
2019-06-15T09:29:15Z
<p>Gnaiger Erich moved page <a href="/index.php/Meszaros_2018_Mitochondrial_Medicine_2018_Hinxton_UK" class="mw-redirect" title="Meszaros 2018 Mitochondrial Medicine 2018 Hinxton UK">Meszaros 2018 Mitochondrial Medicine 2018 Hinxton UK</a> to <a href="/index.php/Meszaros_2018_Mt_Med_Hinxton" title="Meszaros 2018 Mt Med Hinxton">Meszaros 2018 Mt Med Hinxton</a></p>
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Gnaiger Erich
https://wiki.oroboros.at/index.php?title=Meszaros_2018_Mt_Med_Hinxton&diff=180576&oldid=prev
Gnaiger Erich at 09:27, 15 June 2019
2019-06-15T09:27:14Z
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|authors=Meszaros AT, Haider M, Di Marcello M, Gnaiger E</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|authors=Meszaros AT, Haider M, Di Marcello M, Gnaiger E</div></td></tr>
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Gnaiger Erich
https://wiki.oroboros.at/index.php?title=Meszaros_2018_Mt_Med_Hinxton&diff=156709&oldid=prev
Kandolf Georg at 06:52, 20 April 2018
2018-04-20T06:52:06Z
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 06:52, 20 April 2018</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Mitochondrial ''p<sub>50</sub>'' measurement is a quick, highly accurate and sensitive way for early detection of reduced CIV excess capacity, as affinity of mitochondria for O<sub>2</sub> decreases already at a slight CIV inhibition. Determination of mitochondrial O<sub>2</sub> dependence has implications in a wide range of biomedical research topics, particularly in studies with CIV mutations (e.g. Leigh syndrome), or with small inhibitory molecules (nitric oxide). ''O2'''kinetics''''' provides a fast and simple method for the detection of CIV impairment and extends standard OXPHOS analysis to the intracellular O<sub>2</sub> regime ''in vivo'', reflecting low physiological O<sub>2</sub> concentrations or tissue hypoxia.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Mitochondrial ''p<sub>50</sub>'' measurement is a quick, highly accurate and sensitive way for early detection of reduced CIV excess capacity, as affinity of mitochondria for O<sub>2</sub> decreases already at a slight CIV inhibition. Determination of mitochondrial O<sub>2</sub> dependence has implications in a wide range of biomedical research topics, particularly in studies with CIV mutations (e.g. Leigh syndrome), or with small inhibitory molecules (nitric oxide). ''O2'''kinetics''''' provides a fast and simple method for the detection of CIV impairment and extends standard OXPHOS analysis to the intracellular O<sub>2</sub> regime ''in vivo'', reflecting low physiological O<sub>2</sub> concentrations or tissue hypoxia.</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|editor=[[Kandolf G]],</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|editor=[[Kandolf G<ins style="font-weight: bold; text-decoration: none;">]], [[Meszaros AT</ins>]],</div></td></tr>
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Kandolf Georg
https://wiki.oroboros.at/index.php?title=Meszaros_2018_Mt_Med_Hinxton&diff=156708&oldid=prev
Kandolf Georg: Created page with "{{Abstract |title=High-resolution mitochondrial oxygen kinetics as diagnostic tool in complex IV impairments. |authors=Meszaros AT, Haider M, Di Marcello M, Gnaiger E |year=20..."
2018-04-20T06:51:38Z
<p>Created page with "{{Abstract |title=High-resolution mitochondrial oxygen kinetics as diagnostic tool in complex IV impairments. |authors=Meszaros AT, Haider M, Di Marcello M, Gnaiger E |year=20..."</p>
<p><b>New page</b></p><div>{{Abstract<br />
|title=High-resolution mitochondrial oxygen kinetics as diagnostic tool in complex IV impairments.<br />
|authors=Meszaros AT, Haider M, Di Marcello M, Gnaiger E<br />
|year=2018<br />
|event=Mitochondrial Medicine 2018 Hinxton UK<br />
|abstract=Assessment of mitochondrial respiration at higher-than-physiological oxygen (O<sub>2</sub>) concentrations has become a standard in biomedical research. In contrast, the impact of mitochondrial O<sub>2</sub> kinetics on (patho)physiology is largely neglected. To facilitate further studies, we developed the automatized software module ''O2'''kinetics'''''. Using this advanced tool, we re-evaluated the O<sub>2</sub> dependence of mitochondrial respiration in various respiratory states, experimental conditions, and in the presence of cytochrome c oxidase (CIV) inhibitors.<br />
<br />
Mitochondria isolated from mouse brain, heart and liver were incubated at 37 °C in Oroboros O2k High-Resolution FluoRespirometers. Using substrate-uncoupler-inhibitor titration (SUIT) protocols with various fuel substrate combinations in OXPHOS-, LEAK- and ET-states, we investigated the effect of pathway and coupling control on mitochondrial ''p<sub>50</sub>'' (O<sub>2</sub> partial pressure at half-maximum O<sub>2</sub> flux, ''J<sub>O2</sub>''). Kinetic data was obtained during aerobic-anaerobic transitions with high time-resolution at data sampling intervals of 0.2 s. ''p<sub>50</sub>'' values were calculated using the ''O2'''kinetics''''' software for automatic correction and calibration steps. Depending on experimental temperature and respiratory state, ''p<sub>50</sub>'' ranged from 0.006 to 0.07 kPa for NADH-linked LEAK respiration with glutamate&malate, N(GM)''<sub>L</sub>'', and NADH-&succinate-linked OXPHOS capacity including GM and pyruvate, NS(GMP)''<sub>P</sub>'', in agreement with and extending data published previously. In heart and liver mitochondria, the ''p<sub>50</sub>'' was higher in OXPHOS- compared to LEAK-states, increasing proportionally with CIV turnover. Upon inhibition of CIV, ''p<sub>50</sub>'' increased several-fold up to 0.15 kPa, NS(GM)''<sub>P</sub>'', which was not reflected in ''J<sub>O2</sub>'' at kinetic O<sub>2</sub> saturation. In diagnostic experiments, increasing ''p<sub>50</sub>'' values correlated well with decreasing CIV activity, as measured upon addition of ascorbate and TMPD.<br />
<br />
Mitochondrial ''p<sub>50</sub>'' measurement is a quick, highly accurate and sensitive way for early detection of reduced CIV excess capacity, as affinity of mitochondria for O<sub>2</sub> decreases already at a slight CIV inhibition. Determination of mitochondrial O<sub>2</sub> dependence has implications in a wide range of biomedical research topics, particularly in studies with CIV mutations (e.g. Leigh syndrome), or with small inhibitory molecules (nitric oxide). ''O2'''kinetics''''' provides a fast and simple method for the detection of CIV impairment and extends standard OXPHOS analysis to the intracellular O<sub>2</sub> regime ''in vivo'', reflecting low physiological O<sub>2</sub> concentrations or tissue hypoxia.<br />
|editor=[[Kandolf G]],<br />
|mipnetlab=AT Innsbruck Gnaiger E, AT Innsbruck Oroboros<br />
}}<br />
{{Labeling<br />
|area=Respiration, mt-Medicine<br />
|diseases=Neurodegenerative<br />
|organism=Mouse<br />
|tissues=Heart, Nervous system, Liver<br />
|preparations=Isolated mitochondria<br />
|enzymes=Complex IV;cytochrome c oxidase<br />
|topics=Oxygen kinetics<br />
|couplingstates=LEAK, OXPHOS, ET<br />
|pathways=N, CIV, NS<br />
|instruments=Oxygraph-2k<br />
|additional=Labels, 2018-04,<br />
}}<br />
== Affiliations ==<br />
::::Meszaros AT(1), Haider M(2), Di Marcello M(1,3), Gnaiger E(1,3)<br />
:::::#Oroboros Instruments, Innsbruck, Austria<br />
:::::#Steinhauser & Haider Technology Consulting OG, Innsbruck, Austria<br />
:::::#Daniel Swarovski Research Lab, Dept Visceral, Transplant Thoracic Surgery, Medical Univ Innsbruck, Austria. - andras.meszaros@oroboros.at</div>
Kandolf Georg