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Difference between revisions of "MiPNet06.03 POS-calibration-SOP"

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== Further information ==
== Further information ==
:>> [http://www.oroboros.at/?oxygen-solubility  MiPNet06.03]
:>> MiPNet11.08 integrated into MiPNet06.03.
:>> MiPNet11.08 integrated into MiPNet06.03.



Revision as of 08:49, 2 December 2013

Publications in the MiPMap
Gnaiger E (2001) Oxygen calibration and solubility in experimental media. Mitochondr Physiol Network 06.03.

» Open Access-MiPNet06.03.pdf; Versions

O2k-Protocols contents

OROBOROS (2001) Mitochondr Physiol Network

Abstract: O2k-Protocol for oxygen calibration and solubility

High-resolution respirometry critically depends on accurate calibration of the polarographic oxygen sensors which respond to partial oxygen pressure. Expressing the oxygen signal in terms of partial pressure has several advantages, but respiration is expressed in molar units related to biochemical stoichiometries. Conversion of oxygen partial pressure to oxygen concentration requires accurate information on oxygen solubilities in experimental incubation media. Absolute errors up to 10% are commonly found in the established literature. The oxygen solubility of mitochondrial respiration medium MiR06 relative to pure water (oxygen solubility factor, FM) is 0.92, accurately determined (for MiR05, i.e. MiR06 without catalase; MiPNet14.13) at 37 °C and 30 °C. At air saturation at standard barometric pressure (100 kPa) and 37 °C, the partial oxygen pressure is 19.63 kPa, and the oxygen concentration is 190.7 µM in MiR06. A clean and ready-to-use Oxygraph chamber is a requirement for saving time and quickly calibrating the polarographic oxygen sensors.


See also: Oxygen solubility factor and equilibrium concentration of mitochondrial respiration medium MiR06 (i.e. MiR05 + catalase).

cO2* is the oxygen concentration at thermodynamic equilibrium with air under standard conditions of 100 kPa barometric pressure. FM has been determined for MiR05 at 30 °C and 37 °C, and is assumed to be identical at 25 °C.


References

This article is cited in:

Rodriguez JV, Pizarro MD, Scandizzi AL, Guibert EE, Almada LL, Mamprin ME (2008) Construction and performance of a minibioreactor suitable as experimental bioartificial liver. Artif Organs 32:323-328

Forstner H, Gnaiger E (1983) Calculation of equilibrium oxygen concentration. In: Polarographic Oxygen Sensors. Aquatic and Physiological Applications. Gnaiger E, Forstner H (eds), Springer, Berlin, Heidelberg, New York: 321-333.

>> O2k-Protocols:Overall contents
>> Product: OROBOROS Oxygraph-2k, O2k-Catalogue


O2k-Network Lab: AT_Innsbruck_OROBOROS


Labels: MiParea: Instruments;methods 





HRR: Oxygraph-2k, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property. 

O2k-SOP 

Further information

>> MiPNet11.08 integrated into MiPNet06.03.
  • Version 1: 2001
  • Version 11: 2011-12-11

Contents

1 O2k-Chamber: Cleaning and Storage

1.1 Cleaning the Chamber

1.2 Storage in 70% Ethanol

2 Short Two-Point Calibration

3 Equations for Oxygen Calibration

3.1 Oxygen Concentration and Recorded Signal

3.2 Oxygen Pressure and POS Current

3.3 Oxygen Concentration and Oxygen Pressure

3.4 Oxygen Signal and Background Oxygen Consumption

4 O2 Solubility and Concentration at Air Saturation

4.1 Oxygen Pressure and Concentration

4.2 Temperature Effect on Saturation O2 Conc.

4.3 Barometric Pressure Effect on Saturation O2 Conc.

4.4 The O2 Solubility Factor in Salt Solutions