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Difference between revisions of "MiPNet25.06 IOC145 Innsbruck AT"

From Bioblast
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== Preliminary programme ==
== Programme ==
<br>
<div class="toccolours mw-collapsible mw-collapsed">
<span style="font-size:105%; color:#424242">'''Monday 27th'''</span>
<div class="mw-collapsible-content">


::::{| class='wikitable'
[[File:PDF.jpg|100px|link=https://wiki.oroboros.at/images/b/be/MiPNet25.06_IOC145_O2k-Coaching_Days.pdf |Bioblast pdf]]
|-
|style="width: 10em"|
|style="width: 60em"|'''Topic'''
|style="width: 15em"|'''Room'''
|-
|08:15
|Welcome - Get-together: Introduction of participants and their research interests
|Oroboros O2k-Laboratory
|-
|08:30
|OroboPOS service and O2k instrumental setup - overview with videoclips
|Oroboros O2k-Laboratory
|-
|09:15
|'''Hands-on''': OroboPOS service and O2k instrumental setup
|Oroboros O2k-Laboratory
|-
|09:55
|DatLab 7.4 overview
|Oroboros O2k-Laboratory
|-
|10:30
|''Coffee''
|
|-
|11:00
|Instrumental quality control 1: oxygen calibration
|Oroboros O2k-Laboratory
|-
|11:30
|'''Hands-on''': Instrumental quality control 1: oxygen calibration


DL-Protocol: O2k-cleaning BeforeUse
DL-Protocol: O2 calibration air
|Oroboros O2k-Laboratory
|-
|12:00
|''Lunch break''
|
|-
|13:00
|'''Hands-on''': Instrumental quality control 1: oxygen calibration (continuation)
DL-Protocol: O2 calibration air
|Oroboros O2k-Laboratory
|-
|13:30
|Instrumental quality control 2: Instrumental O<sub>2</sub> background – overview with videoclips
|Oroboros O2k-Laboratory
|-
|14:00
|'''Hands-on''':Instrumental quality control 2: Instrumental O<sub>2</sub> background
DL-Protocol: Instrumental O2 background TiP2k
|Oroboros O2k-Laboratory
|-
|15:00
|''Coffee''
|
|-
|15:30-17:30
|'''Hands-on''': Instrumental quality control 1 and 2. DatLab analysis.
|Oroboros O2k-Laboratory
|-
|}
</div>
</div>
<br>
<div class="toccolours mw-collapsible mw-collapsed">
<span style="font-size:105%; color:#424242">'''Tuesday 28th'''</span>
<div class="mw-collapsible-content">
::::{| class='wikitable'
|-
|style="width: 10em"|
|style="width: 60em"|'''Topic'''
|style="width: 15em"|'''Room'''
|-
|08:30
|Hands-on: Instrumental quality control 1: oxygen calibration
DL-Protocol: O2k-cleaning BeforeUse
DL-Protocol: O2 calibration air
|Oroboros O2k-Laboratory
|-
|09:15
|Introduction to substrate-uncoupler-inhibitor titration (SUIT) protocols – fundamental principles. SUIT reference protocol: RP1&RP2
|Oroboros O2k Laboratory
|-
|09:45
|SUITbrowser: how to find the best SUIT protocol for your research questions.
|Oroboros O2k Laboratory
|-
|10:00
|''Coffee''
|
|-
|10:30
|'''Hands-on''': O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with the reference protocol RP1 (SUIT-001) and RP2 (SUIT-002).
DL-Protocol: SUIT-001 O2 ce-pce D003.DLP
DL-Protocol: SUIT-002 O2 ce-pce D007.DLPΒ 
|Oroboros O2k Laboratory
|-
|11:45
|'''Hands-on''': O2k-cleaning after use
DL-Protocol: O2k-cleaning AfterUse
|Oroboros O2k-Laboratory
|-
|12:00
|''Lunch break''
|
|-
|13:00
|'''Hands-on''': O2k-cleaning after use (continuation)
DL-Protocol: O2k-cleaning AfterUse
|Oroboros O2k-Laboratory
|-
|13:30
|DatLab 7.4 analysis: Introduction and new features
|Oroboros O2k-Laboratory
|-
|15:00
|''Coffee''
|
|-
|15:30-17:30
|Individual DatLab analysis + summary
|Oroboros O2k-Laboratory
|-
|}
</div>
</div>
<br>
<div class="toccolours mw-collapsible mw-collapsed">
<span style="font-size:105%; color:#424242">'''Wednesday 29th'''</span>
<div class="mw-collapsible-content">
::::{| class='wikitable'
|-
|style="width: 10em"|
|style="width: 60em"|'''Topic'''
|style="width: 15em"|'''Room'''
|-
|08:30
|'''MitoFit proficiency test'''
'''Hands-on''': Instrumental quality control 1: oxygen calibration
DL-Protocol: O2k-cleaning BeforeUse
DL-Protocol: O2 calibration air
|Oroboros O2k-Laboratory
|-
|10:00
|''Coffee''
|
|-
|10:30
|'''MitoFit proficiency test'''
'''Hands-on''': O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with the reference protocol RP1 (SUIT-001) and RP2 (SUIT-002).Β 
DL-Protocol: SUIT-001 O2 ce-pce D003.DLP
DL-Protocol: SUIT-002 O2 ce-pce D007.DLP
|Oroboros O2k-Laboratory
|-
|11:45
|'''Hands-on''': O2k-cleaning after use
DL-Protocol: O2k-cleaning AfterUse
|Oroboros O2k-Laboratory
|-
|12:00
|Lunch break
|
|-
|13:00
|'''Hands-on''': O2k-cleaning after use (continuation)
DL-Protocol: O2k-cleaning AfterUse
|Oroboros O2k-Laboratory
|-
|13:15
|DatLab analysis
|Oroboros O2k-Laboratory
|-
|14:00
|O2k-Applications - overview
|Oroboros O2k-Laboratory
|-
|14:30
|Proficiency test: DatLab analysis summary
|Oroboros O2k-Laboratory
|-
|15:00
|''Coffee''
|
|-
|15:30
|The Bioblast wiki, MitoFit Preprint Archives, O2k-Network and MitoEAGLE
|MiP''Art''
|-
|16:30
|Feedback & conclusions
|MiP''Art''
|-
|17:00
|Farewell activity
|MiP''Art''
|-
|}
</div>
</div>


== Participants ==
== Participants ==

Revision as of 14:35, 20 January 2020

                



MiPNet25.06 IOC145 Innsbruck AT


Publications in the MiPMap
OROBOROS-solo.png

Innsbruck AT, 2020 Jan 27-29. O2k-Coaching Days - Basic IOC145.


Oroboros (2020-01-27) Mitochondr Physiol Network

Abstract:

O2k-Workshops.png

O2k-Coaching Days - Basic. Innsbruck, Austria; 2020 Jan 27-29.


β€’ O2k-Network Lab: AT_Innsbruck_Oroboros, AT_Innsbruck_MitoFit

Template NextGen-O2k.jpg
O2k-Workshops

General information

The course is fully booked.
O2k-Coaching Days - Basic is a training course which provides a fundamental introduction to high-resolution respirometry (HRR) with the Oroboros O2k. We will give you an overview of the O2k-FluoRespirometer, including real-time analysis with DatLab 7.4 and applications of the Titration-Injection microPump TIP2k. Hands-on sessions range from OroboPOS technical service (instrumental quality control system which is an essential component of HRR) to respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols. Also the new Oroboros SUITbrowser will be presented. The SUITbrowser helps you to find the best SUIT protocol for your specific research questions. Many optimized SUIT protocols are available as DL-Protocols and will be shown at the O2k-Coaching Days.


Venue

Β» Oroboros O2k-Laboratory
Schoepfstrasse 18, 6020 Innsbruck
Β» Information on travel and venue: IOC Innsbruck

Registration


Registration closed


Tutors and lecturers


Programme

Bioblast pdf


Participants

Participant Institution
Participant Banni Aml CZ Hradec Kralove Cervinkova Z - Charles University, Hradec Kralove (CZ)
Participant Blumkin Moriya IL Tirat Hacarmel Minovia - Minovia Therapeutics LTD, Tirat Hacarmel (IL)
Participant Filippi Celine UK London Filippi C - King's College Hospital, London (UK)
Participant Gurkina Angelina AT Innsbruck Oroboros - Oroboros Instruments, Innsbruck (AT)
Participant Handl Jiri CZ Pardubice Rousar T - University of Pardubice, Pardubice (CZ)
Participant Hofmann Julia AT Innsbruck Gnaiger E - DSL-Oroboros Research Lab, Innsbruck (AT)
Participant Huepfl Sebastian AT Innsbruck Oroboros - Oroboros Instruments, Innsbruck (AT)
Participant Micankova Petra CZ Pardubice Rousar T - University of Pardubice, Pardubice (CZ)
Oefele Marlene Oefele Marlene DE Seewiesen Casagrande S - Max Planck Institute for Ornithology, Seewiesen (DE)
Participant Pilotti Andrea IT Udine Grassi B - University of Udine, Udine (IT)
Participant Torres-Quesada Omar AT Innsbruck Gnaiger E - University of Innsbruck, Innsbruck (AT)

Contact

Oroboros Instruments GmbH
High-Resolution Respirometry
Schoepfstrasse 18
A-6020 Innsbruck, Austria
Tel: +43 512 566796
Fax: +43 512 566796 20
E-Mail: [email protected]
Mitochondria and Cell Research


IOC recommended reading

Β» IOC recommended reading
Β» MitoPedia: Respiratory states
OXPHOS ROUTINE ETS LEAK ROX


MitoGlobal
O2k-Workshops and O2k-Coaching Days are listed as MitoGlobal Events.


Labels:






ORO, IOC, MitoGlobal, Next, 2020