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Difference between revisions of "MitoEAGLE protocols, terminology, documentation"

From Bioblast
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::::## ''biological clock'' - '''[[Andric S|Silvana Andric]]''' (2016).
::::## ''biological clock'' - '''[[Andric S|Silvana Andric]]''' (2016).
::::# ''I took a look to the MoU and the different working groups. You know well my research focus and having this into account I am interested in actions related to Working group 1 (Respirometric reference protocols, Interlaboratory proficiency test, Instrumental platforms). Working group 2 and 3: I have generated multiple mitochondrial respiration data sets in different mouse tissues (mainly in skeletal muscle, hypothalamus, liver and white adipose tissue). Currently we are also performing these assays in white adipose tissue from human patients. Hence I am interested in all milestones of WG 2 and 3. WG 5 is related to WG 1 and as you know I have interest in training, dissemination and also together with my collaborators (if needed) we could help with data sets and a data interpretation.  Overall, I have a general interest on this Action and my active role in MITOEAGLE could be discussed. I could be of help participating in some of the tasks where my expertise and interest fit best. We will be forming and interdisciplinary research group with four group leaders: JC Perales, R Guimera, M Sales-Pardo and PM Garcia-Roves with two different affiliations, University of Barcelona (JCP and PMG-R - Energy metabolism) and University Rovira I Virgili (RG and MS – Data base, statistics, data mining).'' - '''[[Garcia-Roves PM|Pablo M Garcia-Roves]]''' (2016).
::::# ''I took a look to the MoU and the different working groups. You know well my research focus and having this into account I am interested in actions related to Working group 1 (Respirometric reference protocols, Interlaboratory proficiency test, Instrumental platforms). Working group 2 and 3: I have generated multiple mitochondrial respiration data sets in different mouse tissues (mainly in skeletal muscle, hypothalamus, liver and white adipose tissue). Currently we are also performing these assays in white adipose tissue from human patients. Hence I am interested in all milestones of WG 2 and 3. WG 5 is related to WG 1 and as you know I have interest in training, dissemination and also together with my collaborators (if needed) we could help with data sets and a data interpretation.  Overall, I have a general interest on this Action and my active role in MITOEAGLE could be discussed. I could be of help participating in some of the tasks where my expertise and interest fit best. We will be forming and interdisciplinary research group with four group leaders: JC Perales, R Guimera, M Sales-Pardo and PM Garcia-Roves with two different affiliations, University of Barcelona (JCP and PMG-R - Energy metabolism) and University Rovira I Virgili (RG and MS – Data base, statistics, data mining).'' - '''[[Garcia-Roves PM|Pablo M Garcia-Roves]]''' (2016).
::::# ''I will be pleased to contribute to the tasks of WG1 by developing  interlaboratory proficiency tests and instrumental platform comparison.'' - '''[[Genova ML| Maria Luisa Genova]]''' (2016).


== Projects in progress ==
== Projects in progress ==

Revision as of 07:40, 23 May 2016


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COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping


MitoEAGLE protocols, terminology, documentation



WG1

Standard operating procedures and user requirement document: Protocols, terminology, documentation


MITOEAGLE Working Groups

WG1 Description

The quality management system, scope of SOPs to be elaborated and the outline of the data repositories will be defined in a user requirement document.

Respirometric reference protocols

A ‘library of protocols’ applied in mitochondrial respiratory physiology will be collected in a standard format, delineating the diversity of experimental approaches to assess bioenergetic function. A set of reference protocols will rigorously document (Maelstrom Research program) the state-of-the-art standards in designing, conducting, reporting, interpreting, and validating such bioenergetic tests (see also Fig. 3).
MITOEAGLE Proficiency ring test

Interlaboratory proficiency test

Inter-laboratory ring tests are a basic requirement for quality management. Such proficiency tests require a reference sample which must be homogenous, stable, representative of the diagnostic target, amenable for distribution, and economical for large-scale production. For respiratory OXPHOS analysis a reference sample of structurally and functionally intact mitochondria is not yet available to perform corresponding ring tests (in contrast to enzymatic OXPHOS ring tests). Recently, the widely applied human cell line HEK 293T was found to be potentially suitable for cryopreservation. Upon thawing the cells can be used immediately for respirometry of both intact and permeabilized cells. Thus interlaboratory proficiency testing may now be feasible as a world-wide innovation in the field of mitochondrial respiratory physiology. Participating labs may implement the test for intra- and interlaboratory validation and longitudinal performance monitoring, using a reference OXPHOS protocol (Fig. 3). The organization of the proficiency test should follow the requirements of the ISO 17043 and 13528 standards. A corresponding MITOEAGLE proficiency training shall be developed and implemented. Feedback from the participants will lead to a final adjustment of the SOPs in the proficiency test, which will be made generally available.

Instrumental platforms

Comparison of results between and within instrumental platforms: The reference protocols and proficiency test will not define the instrumental platform, but will allow a quantitative comparison of results obtained with specific equipment available in the participating laboratories.

Nomenclature

Harmonization of nomenclature on mitochondrial respiratory states and control parameters: The logistics of development of a database requires application of strictly defined terms for all included variables. There is no general reference available upon which a consistent terminology on mitochondrial physiology and bioenergetics can be based. The MITOEAGLE consortium, therefore, has to accomplish the ambitious goal to unify and simplify the terminology in the field for the purpose of the QMS, which will lead to the development of recommendations for the use of a common terminology in mitochondrial physiological research. A publication will be prepared as an Open Access article which will be a milestone towards a unification of concepts and nomenclature.

Knowledge management system

With an increase in data volume and complexity comes along the requirement for standardized report formats and digital accessibility of the data. Since such a database is still missing for mitochondrial physiology, the goal of WG1 is to establish an integrated and analytic environment linking mitochondrial respiration with external data (cell physiology, anthropometry, spiroergometry, lifestyle, genomics) in the context of physical fitness evaluation and clinical diagnostics. Databases will be established combining biological and clinical data comprising proprietary and publicly available datasets into one integrated solution. Data import and export functionalities will allow data upload and mapping to corresponding objects in the database, in this way generating valuable data and allowing for project-specific as well as cross-project data mining.
The ability to formulate meta-studies in our approach is an important success factor: The platform can aggregate the project’s complete knowledge and provides the ability to make diagnostic profiling associated with diseases. In addition to functional data, other data types such as nutritional or environmental factors associated with disease prevention can be identified and aggregated into the MITOEAGLE Knowledge Management System.
Querying the heterogeneous data types and result representation are the most important requirements of the knowledge platform besides to data integration and modelling. The application will feature different types of visualization techniques for all integrated data types. The database will be developed using the concept of multi-tier client-server architecture. Individual components are separated corresponding to their functionality. An upstream web server handles user requests, an application server performing business logic, and a back-end database system guaranties persistent data storage. All components are additionally separated by means of network security incorporating firewalls and are hosted in a secure environment. The platform administration ensures privacy regarding proprietary data by user rights policies.
It will be the task of WG1 to continuously collect the input from all other WGs, compare, discuss and align SOPs as far as possible, and to finally develop recommendations for quality control, data reporting and data sharing beyond the published record. In addition, WG1 will develop concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation. Finally, it will provide a summary on strategic dissemination and an education programme for MITOEAGLE. The education programme will ensure that not only presently participating groups will adhere to the set standards but that also groups joining in at later points may smoothly fit into the community and help to further broaden and deepen our understanding on mitochondrial physiology in health and disease.

WG1 Participants

CountryCityContact
ArmeniaYerevanTrchounian K
AustraliaNedlandsFilipovska Aleksandra
AustraliaSyndeyTon Riccardo
AustraliaVictoriaWinwood-Smith Hugh
AustraliaMelbourneHewakapuge Sudinna
AustraliaMelbourneLionett S
AustraliaClayton VictoriaAlton Lesley
AustraliaMelbourneCoughlan Melinda T
AustraliaSydneyHoehn Kyle L
AustraliaMelbourneCassar Samantha
AustraliaMelbourneGenders Amanda J
AustraliaQueenslandRadenkovic Filip
AustraliaSydneyKrycer James R
AustraliaQueenslandFisher Joshua J
AustraliaMelbourneStepto Nigel K
AustraliaClaytonWolff Jonci Nikolai
AustraliaNewtown, SydneyStocker Roland
AustraliaQueenslandHolland Olivia J
AustraliaGeelong, VictoriaMcKenzie Matthew
AustraliaMelobourneBotella Javier
AustraliaQueenslandDong Lan-Feng
AustraliaSouthportVidimce Josif
AustraliaMelbourneFerri Alessandra
AustraliaVictoriaVan Bergen Nicole J
AustraliaNedlandsPerks Kara L
AustraliaMelbourneWhitfield Jamie
AustraliaBurwoodTrewin Adam J
AustraliaMelbourneSaner Nicholas
AustraliaMelbourne, VICMuccini Anna Maria
AustraliaMelbourneKuang Jujiao
AustraliaParkvilleHardee Justin P
AustriaInnsbruckSchwarzer Christoph
AustriaAldransHaider Markus
AustriaInnsbruckHoppel Florian
AustriaKlosterneuburgSazanov Leonid A
AustriaInnsbruckDoerrier Carolina
AustriaInnsbruckNindl Veronika
AustriaInnsbruckSobotka Ondrej
AustriaInnsbruckGarcia-Souza Luiz F
AustriaInnsbruckKeller Markus A
AustriaInnsbruckLaner Verena
AustriaInnsbruckGnaiger Erich
AustriaInnsbruckGoebel G
AustriaInnsbruckPlangger Mario
AustriaInnsbruckThapa Maheshwor
AustriaInnsbruckMeszaros Andras T
AustriaInnsbruckSchartner Melanie
AustriaInnsbruckPassrugger Manuela
AustriaSalzburgKofler B
AustriaInnsbruckBergmeister Lisa
AustriaInnsbruckKlocker Helmut
AustriaInnsbruckDi Marcello Marco
AustriaViennaKozlov Andrey V
AustriaInnsbruckAntunes Diana
AustriaInnsbruckKarabatsiakis Alexander
AustriaKapferer Werner
AustriaInnsbruckBurtscher Martin
AustriaInnsbruckSchmitt Sabine
AustriaInnsbruckCecatto Cristiane
AustriaInnsbruckGallee Leon
AustriaInnsbruckCardoso Luiza HD
AustriaInnsbruckPerez Valencia Juan Alberto
BelgiumBrusselsSonveaux P
BelgiumLeuven, FlandersBaekelandt V
BelgiumNamurArnould Thierry
BelgiumLiegeVotion Dominique-Marie
BelguimSart Tilman (Liège)Kruse C
BrasilPorto AlegreHaas Clarissa
BrasiliaLimeiraRopelle Eduardo R
BrazilJaboticabalOliveira Marcos Tulio
BrazilRio de JaneiroGalina Antonio
BrazilRio de JaneiroOliveira Marcus F
BrazilSao PauloSilber Ariel Mariano
BrazilSão PauloCrispim Marcell
BrazilSao PauloVercesi Anibal E
BrazilRio de JaneiroVieyra Adalberto
BrazilSanta Maria - RSGoncalves Debora Farina
BrazilSanta Maria - Rio Grande do SulSoares Felix Alexandre Antunes
BrazilSão PauloKowaltowski Alicia J
CanadaMoncton, NBPichaud Nicolas
CanadaAntigonishWeir M
CanadaVancouverBovard Josh
CanadaEdmonton (Alberta)Lemieux Helene
CanadaEdmontonZaugg Michael
CanadaRimouskiMunro Daniel
CanadaMontréalBreton Sophie
CanadaHamiltonScott Graham R
CanadaMontreal, QuebecBergdahl Andreas
CanadaOttawaHarper Mary-Ellen
CanadaEdmontonLucchinetti Eliana
CanadaHamiltonTarnopolsky Mark A
CanadaSaint JohnPulinilkunnil Thomas
CanadaGuelphHolloway Graham P
CanadaOttawaDarveau Charles-A
CanadaEdmontonHan Woo Hyun
CanadaVancouver, BCTausan Daniel
CanadaQuebecJoseph Vincent
CanadaWinnipegChowdhury Subir Roy
CanadaCharlottetownAdiele Reginald C
CanadaRimouskiBlier Pierre U
ChileCoyhaiqueJana Prado Fabian
ChinaFuzhou, Fujian ProvinceChen Qi
ChinaTianjinZhang Yong
ChinaXi`anLiu Jiankang
ChinaPukou, Nanjing,Gan Zhenji
CroatiaZagrebSaric A
CroatiaZagrebCurik I
CroatiaZagrebRubelj Ivica
CroatiaSplitLjubkovic M
Czech RepublicHradec KraloveKucera Otto
Czech RepublicVestecDavidova Eliska
Czech RepublicPrague 10Urban Tomas
Czech RepublicHradec KraloveStankova Pavla
Czech RepublicHradec KraloveCervinkova Zuzana
Czech RepublicPlzen -Severni predmestiJedlicka Jan
Czech RepublicPardubiceMajtnerova Pavlina
Czech RepublicCeske BudejoviceZikova Alena
Czech RepublicPardubiceRousar Tomas
Czech RepublicPragueDrahota Zdenek
Czech RepublicPardubiceHandl Jiri
Czech RepublicPraha 2Danhelovska Tereza
Czech RepublicPragueKrizova J
Czech RepublicPragueHoustek Josef
Czech RepublicVestecRohlena Jakub
Czech RepublicHradec KraloveEndlicher Rene
Czech RepublicVestecEzrova Zuzana
Czech RepublicPraha 2Zdrazilova Lucie
Czech repHradec KraloveFalaye T
DenmarkCopenhagen NMaise Chroeis K
DenmarkCopenhagen NMortensen OH
DenmarkRoskildeDalgaard Louise T
DenmarkAarhus NJespersen Nichlas Riise
DenmarkCopenhagenDela Flemming
DenmarkCopenhagenNielsen B
DenmarkAarhus NBoetker Hans Erik
DenmarkHvidovreNehlin Jan O
DenmarkCopenhagenLarsen Steen
DenmarkCopenhagenGonzalez-Franquesa Alba
DenmarkCopenhagenKraunsoee R
DenmarkCopenhagen NScheibye-Knudsen Morten
DenmarkCopenhagenSoendergaard SD
EgyptCairoAbdel-Rahman Engy Ali
EgyptCairoAli Sameh S
EstoniaTallinnTruu Laura
EstoniaTallinnVendelin Marko
EstoniaTallinnKlepinina Lyudmila
EstoniaTartuPaju Kalju
EstoniaTallinnKaambre Tuuli
EstoniaTallinnTepp Kersti
EstoniaTallinnPuurand Marju
EstoniaTallinnKlepinin Aleksandr
EstoniaTallinnLaasmaa Martin
EstoniaTallinnShevchuk Igor
EstoniaTallinnKarro N
FinlandJyvaeskylaeKainulainen Heikki
FinlandHelsinkiSuomalainen Wartiovaara Anu
FinlandTurkuStier Antoine
FinlandHelsinkiJackson Christopher Benjamin
FranceMaisons-Alfort CedexProla Alexandre
FranceAngersSpinazzi Marco
FranceNouzillyCollin-Chenot Anne
FranceMartiniqueNeviere Remi
FranceMontpellierWrutniak-Cabello Chantal
FranceParisBouillaud Frederic
FranceLilleMontaigne David
FranceMartiniqueCano Sanchez Maria Consolacion
FranceBordeauxAmoedo Nivea Dias
FranceGueguen Naig
FranceBordeauxSarlak Saharnaz
FranceParisArmand Anne-Sophie
FranceBordeaux cedexMourier A
FranceAngersProcaccio Vincent
FranceBordeaux cedexRossignol Rodrigue
FrancePlouzanéSalin Karine
FranceToursDumas Jean-Francois
GermanyDuesseldorfHaendeler Judith
GermanyRostockSokolova Inna
GermanyGießenSilaidos Carmina
GermanyWilhelmshavenSalmon Pablo
GermanyDoermann Niklas
GermanyGiessenSommer Natascha
GermanyFrankfurt am MainViel Christian
GermanyFrankfurtOsiewacz Heinz D
GermanyNeuherbergEiner C
GermanyBiberach an der RißNold V
GermanyDuesseldorfJelenik T
GermanyMainzMaull Felicia
GermanyTuebingenKappler Lisa
GermanyFrankfurtWarnsmann Verena
GermanyColognePesta Dominik
GermanyGiessenSchulz Rainer
GermanyMainzMethner Axel
GermanyDuesseldorfPiel Sarah
GermanyCologneHo Dieu Hien
GermanyUlmSchaefer PM
GermanyRegensburgRenner-Sattler Kathrin
GermanyHannoverDas Anibh Martin
GermanyJenaSzibor Marten
GermanyCologneMaciej Sarah
GermanyBremerhavenMark Felix Christopher
GermanyMunichZischka Hans
GermanyMagdeburgSchoenfeld Peter
GermanyMarburgVogt Sebastian
GermanyCologneWiesner Rudolf J
GermanyFrankfurtHamann Andrea
GermanyGießenGrewal Rekha
GermanyDüsseldorfGoy C
GermanyMarburgRamzan Rabia
GermanyWickert Anika
GermanyDuesseldorfRoden Michael
GermanyFreiburgSchuele R
GermanyCologneTrifunovic Aleksandra
GreeceHeraklionTavernarakis Nektarios
GreeceAthensTrougakos Ioannis P
GreeceAthensGumeni Sentiljana
Greece15771Andreadou Ioanna
GreeceThessalonikiLazou Antigone
HungarySzegedBoros Mihaly
HungaryBudapestHorvath G
HungaryBudapestChinopoulos Christos
HungaryDebrecenVerebne Tar K
HungaryBudapestTretter Laszlo
HungaryBudapestKomlodi Timea
HungarySzegedFerdinandy Peter
IndiaHyderabadJha Rajan Kumar
IndiaNew DelhiThakkar Himani
IndiaNew DelhiVincent Vinnyfred
IndiaLucknowGayen Jiaur
IndiaHaryanaChakrabarti Sasanka
IndiaKollamSuravajhala Prashanth
IndiaVaranasiSonkar Vijay K
IranTehranSafaei Zahra
IranTehranShirazi Reza
IrelandDublinO'Gorman Donal
IrelandDublinPorter Richard K
IsraelJerusalemSaada Reisch Ann
IsraelRishon Le ZionHachmo Yafit
IsraelZrifinSova Marina
IsraelBeer ShevaMishmar Dan
ItaliaUdineSalvadego Desy
ItalienPadova PDSzabo Ildiko
ItalyBolognaGasparre G
ItalyBolzanoZanon Alessandra
ItalyBolzano/BozenVolani Chiara
ItalyCataniaMessina Angela
ItalyPadovaBernardi Paolo
ItalyBariAttimonelli M
ItalyCataniaDe Pinto Vito
ItalyPadovaFernandez-Vizarra Erika
ItalyMonserratoIsola Raffaella
ItalySt. LorenzenHarrison David K
ItalyCataniaMagri Andrea
ItalyCampobassoPallotta Maria Luigia
ItalyUdineGrassi B
ItalyBolognaGenova Maria Luisa
ItalyRomeScatena Roberto
ItalyMilanoBottoni P
ItalyBolognaPaterlini S
ItalyBolognaPorcelli AM
ItalyMilanClementi Emilio
ItalyAnconaBattino Maurizio
ItalyPadovaViscomi Carlo
JapanItabashi-ku TokyoTanaka Masashi
JapanSapporoMatsumoto J
KitzbühelInnsbruckFischer Michael J
KoreaSeoulPak Youngmi Kim
KoreaIncheonKwak Hyo Bum
Korea (South)SeoulLee Hong Kyu
LatviaRigaInashkina I
LatviaRigaJansone Baiba
LatviaRigaDambrova Maija
LatviaRigaVilks Karlis
LatviaRigaSvalbe Baiba
LatviaRigaZvejniece Liga
LatviaRigaLiepins Edgars
LatviaRigaStelfa Gundega
LatviaRigaVolska Kristine
LuxembourgStrassenDevaux Yvan
MalaysiaKuala LumpurHassan Hazirah
MaltaMsidaVella Joanna
MexicoMexico CityAparicio Trejo OE
MexicoTlalpanRodriguez-Enriquez Sara
MéxicoSan Pedro Garza García, Nuevo LeonGarcia-Rivas Gerardo
NetherlandsGA WageningenGrefte Sander
NetherlandsMaastrichtGrevendonk L
New ZealandAucklandHickey Anthony J
New ZealandAucklandWard Marie Louise
New ZealandWellingtonBerridge Michael V
New ZealandAucklandKaur Sarbjot
NorwayTrondheimRognmo O
NorwayTromsoeBoardman Neoma T
NorwayBergenBlindheim Dan Filip
NorwayBergenDyrstad Sissel E
NorwayTrondheimLerfall Joergen
NorwayBergenPettersen Nitschke Ina Katrine
NorwayTrondheimGrill V
NorwayBergenBerge Rolf K
NorwayBergenRoesland Gro Vatne
NorwayBergenHoel Fredrik
NorwayOsloEide L
NorwayBergenHaavik Jan
NorwayTrondheimSmenes Benedikte Therese
NorwayBergenTronstad Karl Johan
PolandWarsawDymkowska Dorota
PolandWarsawWieckowski Mariusz R
PolandLodzLabieniec-Watala Magdalena
PolandKrakowDembinska-Kiec Aldona
PolandWarsawKampa Rafal Pawel
PolandWarszawaJaskiewicz Anna
PolandWarsawSzewczyk Adam
PolandWarsawBednarczyk Piotr
PolandPoznanJarmuszkiewicz Wieslawa
PolandGdańskKaczor Jan Jacek
PolandWarsawSek Aleksandra
PolandWarsawKarkucinska-Wieckowska Agnieszka
PolandWarsawZablocki Krzysztof
PolandWarsawWasilewski M
PolandLodzWatala Cezary
PortugalCoimbraGrilo Luis
PortugalCoimbraOliveira Paulo J
PortugalCoimbraMinuzzi Luciele M
PortugalLisboaMacedo Maria Paula
PortugalCoimbraPalmeira Carlos
PortugalAveiroFerreira Rita Maria P
PortugalPortoOliveira Jorge
PortugalPortoOliveira Pedro Fontes
PortugalCantanhedeTeodoro Joao Soeiro
PortugalCantanhedeRodrigues Ana Sofia
PortugalAveiroVitorino Rui Miguel Pinheiro
PortugalPortoCrisostomo Luis
PortugalPortoMoreira Bruno P
PortugalCoimbraSantos Diana
PortugalPortoSilva Ana Maria
PortugalCantanhedeAmorim Ricardo
PortugalCoimbraDias Candida
PortugalPortoBeleza Jorge
PortugalCoimbraDuarte Filipe Valente
PortugalPortoAlves Marco G
PortugalCoimbraRolo Anabela Pinto
QatarDohaGuarch Meritxell Espino
Republic of GhanaCape CoastTei BN
Republic of KoreaDaejeonPark SH
Republic of KoreaBusanKim Hyoung Kyu
Republic of KoreaBusanHan Jin
Republic of KoreaYuseong-gu DaejeonQuang Don T
Republic of SerbiaNovi SadAndric Silvana
Republic of SerbiaBelgradeLalic Nebojsa M
Republic of SerbiaBelgradeKrako Jakovljevic Nina
RussiaMoscowPavlova Nadia
RussiaYoshkar-OlaDubinin M
RussiaMoscowVinogradov Andrey D
SerbiaBelgradeMandic M
SerbiaBelgradeDjordjevic M
SerbiaBelgradeSavkovic U
SerbiaBelgradeMisirkic Marjanovic Maja
SingaporeSingaporeSingh Brijesh Kumar
SingaporeSingaporeHausenloy Derek J
SlovakiaBratislava 45Ferko Miroslav
SlovakiaKošiceCizmarova Beata
SlovakiaBratislavaSumbalova Zuzana
SpainSevillaBustos Matilde
SpainMadridHernansanz-Agustin Pablo
SpainBarcelonaDe la Torre Lara J
SpainA CoruñaMayan MD
SpainSalamancaAlmeida Angeles
SpainMadridRial Eduardo
SpainMadridAragones Lopez J
SpainLas Palmas de Gran CanariaCalbet Jose AL
SpainArmilla (Granada)Fernandez-Ortiz Marisol
SpainBarcelonaGoncalo Teixeira da Silva Rui
SpainA CoruñaBlanco FJ
SpainGranadaHidalgo-Gutierrez A
SpainBarcelonaBravo-Sagua Roberto
SpainMadridMoran M
SpainBarcelonaPerales Jose Carles
SpainValenciaCasado Pinna Marta
SpainBarcelonaZorzano Antonio
SpainBarcelonaGil J
SpainBarcelonaGama Perez Pau
SpainGranadaQuiles JL
SpainBarcelonaGarcia-Roves Pablo Miguel
SwedenStockholmShabalina Irina G
SwedenGothenburgBhuvanachandran NS
SwedenLundEhinger Johannes K
SwedenStockholmNedergaard J
SwedenStockholmCardinale Daniele A
SwedenStockholmLarsen Filip J
SwitzerlandZürichZaugg Kathrin
SwitzerlandLausanneWeger M
SwitzerlandBaselBouitbir Jamal
SwitzerlandLausanneZanou Nadege
SwitzerlandLausanneZalachoras I
SwitzerlandLausanneSandi Carmen
SwitzerlandLausanneAmati Francesca
SwitzerlandLausanneCanto Alvarez Carles
SwitzerlandLausanneGebara E
SwitzerlandBernWyss RK
SwitzerlandZurichGorr Thomas A
SwitzerlandLausanneLagarrigue Sylviane
SwitzerlandBaselFornaro Mara
SwitzerlandLausanneBurtscher Johannes
SwitzerlandLausanneMorato Fornaguera L
SwitzerlandLausannePlace Nicolas
SwitzerlandBernNuoffer Jean-Marc
SwitzerlandLausanneDonnelly Chris
TaiwanChanghua CityWei Yau-Huei
The NetherlandsMaastrichtSchrauwen Patrick
The NetherlandsBT AmsterdamWuest Rob CI
The NetherlandsMaastrichtNabben Miranda
The NetherlandsDelftMcMillan Duncan GG
The NetherlandsGroningenBakker Barbara M
UKCambridgeBrown Guy C
UKLondonBettinazzi Stefano
UKCanterbury, KentGourlay Campbell W
UKLondonRodriguez Enrique
UKLondonLane Nick
USADentonDzialowski Edward M
USAPittsburghProchownik Edward V
USAGalvestonDurham William J
USAOrlandoCoen Paul M
USAAuroraFord Ellen
USALouisvilleMenze Michael A
USAAnn ArborTyrrell Daniel J
USAMilwaukeeDash Ranjan K
USADenverVan Hove Johan
USAWarminsterOrynbayeva Zulfiya
USACorvallisNewsom Sean A
USAOrlandoGoodpaster Bret H
USAOmahaKim Julian KS
USAColumbusSims Carrie A
USAPortlandGarlid Keith D
USAAuroraSparagna Genevieve C
USACambridgeWohlwend Martin
USAEl PasoBajpeyi Sudip
USANeedhamBrown David A
USABethesdaSharma Pushpa
USAMiamiFontanesi F
USAFairbanksCoker Robert H
USABostonKristal Bruce S
USAPuskarich Michael A
USAClevelandTandler Bernard
USAStanfordStary Creed
USADurhamLi Pingan Andy
USABerkeleyWilliams Caroline M
USAOrlandoPino Maria F
USALittle RockBorsheim Elisabet
USALittle RockMacMillan-Crow Lee Ann
USALa JollaValentine Joseph Marco
USAFort CollinsChicco Adam J
USALa JollaSchilling Jan M
USATorranceRossiter Harry B
USAAuroraMoreau Kerrie
USAClevelandHoppel Charles L
USACorvallisRobinson Matthew M
USAGainesvilleNeyroud D
USABethesdaFessel Joshua Patrick
USAOrlandoSparks Lauren M
USARochesterNair K Sreekumaran
USAGainesvilleLeeuwenburgh Christiaan
USAMilwaukeeKandel Sunil Mani
USALouisvilleSkolik Robert A
USALittle RockParajuli Nirmala
USASeattleMarcinek DJ
USANew HavenCavalcanti-de-Albuquerque Joao Paulo
USAWakefieldVarricchio Frederick
USAIndianapolisBrozinick Joseph T
USAPhiladelphiaTowheed Atif
USAFort CollinsLi Puma Lance C
USANorfolkLai Nicola
USANew YorkGalkin Alexander
USAPhiladelphiaTomar Dhanendra
USAEast LansingBazil Jason N
USAAtlantaJang Young Charles
USAKansas CityThyfault John P
USARochesterLanza Ian R
USASt. LouisAbumrad Nada A
USAChicagoDayanidhi Sudarshan
USAWinston-SalemAhn Bumsoo
USAKansas CitySwerdlow Russell H
USAColorado SpringsJacobs RA
USAWinston-SalemGonzalez-Armenta Jenny L
USAIowa CityChaurasia Bhagirath
USAFairbanksO'Brien Kristin
USACorvallisBatterson Philip M
USAStillwaterDavis Michael S
USABirminghamMoellering Douglas R
USAFayettevilleIyer Shilpa
USABaltimoreGonzalez-Freire Marta
USASan Diego; La JollaMolina Anthony JA
USASan DiegoPatel Hemal H
USABaton RougeIrving Brian A
USASt. LouisPietka Terri A
USAPhiladelphiaJang David H
USAFayettevilleRao RR
USADavisAdams Sean H
USABaton RougeHand Steven C
USANashvilleWasserman David H
USAAkronCohen Bruce H
USAIowa CityWagner Brett A
USAEast LansingZhang Yizhu
... further results

WG1 Management

Tasks

  1. Opening of the database to the research public for data search and moderated entry of new data sets.
  2. Development of interlaboratory proficiency ring test, of recommendation/certification reports and of a MITOEAGLE proficiency training module (with WG5).
  3. Sample distribution, ring test experiments (MITOEAGLE-proficiency training on two different points of time, 6 months apart), data analysis by experimenters and MITOEAGLE-proficiency test manager.
  4. Reports and publication: Reports with specific results in comparison with an anonymous data summary, and joint publication. Individual reports may be complemented by recommendations, and are summarized in an informal certification report (move to WG5).
  5. Instrumental platform comparison with reference sample applying comparable measurements.
  6. Draft, dissemination, collection and final formulation of a manuscript on concepts and terminology of mitochondrial physiology (with WG5).
  7. Collect and discuss procedures and experimental protocols for the evaluation of mitochondrial capacities and create a library of protocols: A database comprising reference SUIT protocols, standard experimental media, and detailed instructions for OXPHOS analysis is assembled.
  8. MITOEAGLE data management system (DMS) development: The DMS for further data preprocessing. The DMS will enable management and analysis of instrument-specific and project-specific data.
  9. MITOEAGLE database (DB) development: DB will facilitate high-level project specific analysis as well as meta-analyses across projects, visualization of project specific data as well as integrative analyses of datasets for various proprietary and/or publicly available datasets.
  10. Testing of developed software components regarding functionality and usability, modification and implementation. Deployment for routine testing in the labs of our strategic partners.
  11. Data input: Upload of datasets generated through WG 2-4 in internal lab developments, results of the proficiency ring test, and during application module developments. In addition, the database will be populated with available proprietary and public datasets.
  12. Develop recommendations for quality control, data reporting and data sharing beyond the published record. Develop concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation (move to WG5).
  13. Provide a summary on strategic dissemination and an education programme for MITOEAGLE (move to WG5).


Milestones

  1. Data sets entered in standardized format and database opened for the public
  2. Protocols for ring-tests and standard analysis and report scheme defined
  3. MITOEAGLE proficiency training workshop finished (move to WG5)
  4. Analysis is completed and joint publication submitted (with WG5)
  5. Publication of paper on concepts and terminology (with WG5)
  6. Library of protocols online
  7. Publication of recommended protocols and procedures
  8. Guidelines for acquisition, evaluation and data documentation available (with WG5)
  9. MITOEAGLE data management system finished
  10. MITOEAGLE database ready for use
  11. Software modifications incorporated following extensive tests
  12. Publication of MITOEAGLE-KMP
  13. Recommendations on data acquisition, evaluation and management published (with WG5)
  14. Concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation published (move to WG5)


Deliverables

  1. Guidelines for future research and recommendations for the evaluation of respiratory characteristics in human respirometric samples (with WG5)
  2. A database on mt fitness evaluated with human cells and tissues
  3. Training of researchers towards improved reproducibility of sample preparation and respirometric evaluation (move to WG5)
  4. Qualitative and quantitative evaluation of results obtained in ongoing studies by comparison with a reference sample
  5. An expanded MITOEAGLE database
  6. A joint publication documenting the results of the MITOEAGLE-PT (with WG5)
  7. A multi-authored publication towards a unification of concepts and nomenclature in mitochondrial physiology (with WG5)
  8. A library of protocols for sample preparation and examination by respirometry
  9. A multi-authored publication with recommendations of comparable standard protocols and procedures (with WG5)
  10. Guidelines as to the optimum use of a reference sample for determination of respiration and related parameters of interest
  11. The MITOEAGLE data management system
  12. A continuously updated MITOEAGLE database for the use as reference data and guideline towards application of optimized protocols, ultimately leading towards standardization.
  13. A joint publication on the use of the MITOEAGLE database and management system (with WG5)
  14. An education programme for the use of MITOEAGLE database and management system (move to WG5).

WG1 Feedback

  1. We are mostly interested in two WG: (1) Since we are working mostly with heart and muscles, we are interested in WG 2 - MITOEAGLE data repository in muscle and other tissues. (2) WG 1 - Standard operating procedures and user requirement document: Protocols, terminology, documentation. Since we have an expertise in fatty acid metabolism, we are specially interested in experimental protocols for the evaluation of mitochondrial capacities regarding fatty acid metabolism. Of course our parts of the WG are also in our interest. - Marina Makrecka-Kuka, Edgars Liepinsh (2016).
  2. My role in Mito-EAGLE would be mainly in WG 2 and 4, with a focus on skeletal and cardiac muscle and cultured cardiomyocytes and endothelial cells. I would particularly like to bridge the gap between the group leaders and young investigators, trying to represent the young people who are the active users of the OROBOROS equipment. This will fit in nicely with the goals in WG1. ... Major congratulations on obtaining this beautiful collaborative grant! Very glad to see this network getting the recognition it deserves! Looking forward to being part of this! - Rob C Wuest (2016).
  3. Please, find enclosed my declaration to participate with my team in the following workgroups: WG1, WG2, WG3, WG4. We are now involved and have been in not a distant past – in studying of the following cell /tissue types:
    1. Blood cells, with particular emphasis on platelets and lymphocytes – isolated from animals and humans.
    2. Endothelial cells, fibroblasts, neurocytes (neuroblastoma), astrocytes, cancer cell lines – predominantly cultured.
    3. Hepatocytes, cardiomyocytes, cell isolated from brain – isolated from lab animals. - Cezary Watala (2016).
  4. I have already contacted the portuguese CNC to nominate me for the MC of theaction. Concerning my active participation, taking into account the type of work we do in my lab I could be included in all WGs. As you know we work with mitochondria from liver, muscle and fat, as well as cultured cells from different origins (WG2-4). The elaboration of SOPs (WG1) we can certainly contribute (see the book I edited on Mitochondrial Bioenergetics and in which you contribute a chapter). I believe that everybody can be part of the WG5, but as the coordinators of the action, you and Sandra should decide what is best for the success of the action. - Carlos Palmeira (2016).
  5. I would propose my participation in the WG1: Standard operating procedures and user requirement document: Protocols, terminology, documentation, considering that the goal of WG1 is to establish an integrated and analytic environment linking mitochondrial respiration with external data (cell physiology, anthropometry, spiroergometry, lifestyle, genomics) in the context of physical fitness evaluation and clinical diagnostics. I could give my active contribution in the phase of making platform that will aggregate the Action’s complete knowledge and provides the ability to make diagnostic profiling associated with diseases in particular tasks:
    1. Develop reccomendations for quality control, data reporting and data sharing beyond the published record. Develop concepts on Open Access and institutionalised service for data management, data mining and health-care conforming standards of data interpretation
    2. Provide a summary on strategic dissemination and an education programme for MITOEAGLE.
    3. Considering that we are in process of buying the oxygraph, thus broading capacities of my lab, I would be happy to open the door of my new lab for the innovative inter-laboratory ring test experiments and together with my team contribute in that phase of the MITOEAGLE Action. I look forward to the establishment of MitoGlobal EAGLE network where, as stated in the MoU, researchers will collaborate on mapping mitochondrial physiology and medicine. The creation of such a unique well-coordinated network of senior researchers and young investigators will definitely put an added value to existing data and publications, will increase quality and validity of the research, will bring new possibilities of data sharing and data mining and will hopefully bring us to the era of more economical research by saving resources through standardised and harmnonised protocols, where the measuremnts will not be wasted due to lack of comparability. - Nebojsa Lalic (2016).
  6. Our research interest is oriented to reproductive endocrinology; especially to mechanisms that govern steroidogenesis of testicular Leydig cells in disturbed homeostasis. We use several in vivo models:
    1. stress -/+ systemic or intratesticular blockade of different stress receptors (alpha1ADR; betaADR; GR)
    2. hypogonadal-hypogonadism -/+ testosterone-replacement therapy
    3. androgens anabolics treatment -/+ blockade od AR
    4. aged rats -/+ Viagra-treatment
    5. biological clock - Silvana Andric (2016).
  7. I took a look to the MoU and the different working groups. You know well my research focus and having this into account I am interested in actions related to Working group 1 (Respirometric reference protocols, Interlaboratory proficiency test, Instrumental platforms). Working group 2 and 3: I have generated multiple mitochondrial respiration data sets in different mouse tissues (mainly in skeletal muscle, hypothalamus, liver and white adipose tissue). Currently we are also performing these assays in white adipose tissue from human patients. Hence I am interested in all milestones of WG 2 and 3. WG 5 is related to WG 1 and as you know I have interest in training, dissemination and also together with my collaborators (if needed) we could help with data sets and a data interpretation. Overall, I have a general interest on this Action and my active role in MITOEAGLE could be discussed. I could be of help participating in some of the tasks where my expertise and interest fit best. We will be forming and interdisciplinary research group with four group leaders: JC Perales, R Guimera, M Sales-Pardo and PM Garcia-Roves with two different affiliations, University of Barcelona (JCP and PMG-R - Energy metabolism) and University Rovira I Virgili (RG and MS – Data base, statistics, data mining). - Pablo M Garcia-Roves (2016).
  8. I will be pleased to contribute to the tasks of WG1 by developing interlaboratory proficiency tests and instrumental platform comparison. - Maria Luisa Genova (2016).

Projects in progress

Interlaboratory proficiency ring test
» MitoFit proficiency test
Testing of software components
» MiPNet21.07 MitoFit DatLab proficiency test
Concepts and terminology on mitochondrial physiology
» MitoPedia: Respiratory states
» MitoPedia: Respiratory control ratios
» MitoPedia: SUIT
Procedures and experimental protocols for the evaluation of mitochondrial capacities, library of protocols
» MitoPedia: Sample preparations
» MitoPedia: Media for respirometry
» MitoPedia: Substrates and metabolites
» MitoPedia: SUIT
» MiPNet21.06 SUIT reference protocol
» MitoFit-QCS
Data management system (DMS)
» MitoFit-KMP
Database
» MitoFit Quality Control System
» MiPMap


Next steps

Best practices for implementation of analytical and process standards.


References

» ISO/IEC 17043:2010 General requirements for proficiency testing
» ISO 13528:2015 Statistical methods for use in proficiency testing by interlaboratory comparison
» ISO 15189:2012 Medical laboratories — Particular requirements for quality and competence
» ISO 17511:2003 In vitro diagnostic medical devices
» ISO 9001:2015 Quality management systems - requirements
» ISO 10012:2003 Measurement management systems
General requirements for the competence of testing and calibration laboratories » ISO/IEC 17025:2005