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Difference between revisions of "MitoKit-CII/Malonate-nv"

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{{MitoPedia
{{MitoPedia
|abbr=Mnanv
|abbr=Mnanv
|description='''NV161/MitoKit-CII''' (diacetoxymethyl malonate) is a plasma membrane-permeable (p) prodrug that diffuses across the plasma membrane. Cleavage of diacetoxymethyl residues is mediated by intracellular esterases, thus releasing malonate in the intracellular space.
|description='''MitoKit-CII/Malonate-nv''' (diacetoxymethyl malonate) is a plasma membrane-permeable prodrug (permeable malonate; Mnanv) that diffuses across the plasma membrane. Cleavage of diacetoxymethyl residues is mediated by intracellular esterases, thus releasing [[malonate]] in the intracellular space. Abliva #: 01-161-s2
|info=[[MitoKit-CII]], [[MitoKit-CII MitoPedia]], [[Ehinger 2016 Nat Commun]]
|info=[[MitoKit-CII]], [[Ehinger 2016 Nat Commun]]
}}
 
{{MitoPedia topics
|mitopedia topic=Medium
}}
}}


__TOC__
== Application in HRR ==
== Application in HRR ==
:::: '''Mnanv: NV161''' 10 mg, 7.7 µL, 5.24 M, MW=248.1 g.mol<sup>-1</sup>
::: '''Malonate-nv:''' 10 mg, 7.7 µL, 5.24 M, MW=248.1 g.mol<sup>-1</sup>
:::: '''Preparation of 100 mM stock solution:'''
:::: '''Preparation of 100 mM stock solution:'''
::::# Add 395.78 µL DMSO in 7.7 µL NV161.
::::# Add 395.78 µL DMSO in 7.7 µL NV161.
::::# Divide into 40 µL portions.  
::::# Divide into 40 µL portions.  
::::# Store at -20 °C.
::::# Store up to 6 months at -20 °C or up to 1 week at room temperature.


:::: '''Preparation of 10 mM stock solution (from the 100 mM stock):'''  
:::: '''Preparation of 10 mM stock solution of Mnanv (from the 100 mM stock):'''  
::::# Dilute 40 µL 100 mM stock solution with 360 µL DMSO.
::::# Dilute 40 µL 100 mM stock solution with 360 µL DMSO.
::::# Divide into 100 µL portions.   
::::# Divide into 100 µL portions.   
::::# Store at -20 °C.
::::# Store up to 6 months at -20 °C or up to 1 week at room temperature.


:::» '''O2k manual titrations'''  
:::» '''O2k manual titrations'''
::::* Event name: Mnanv
::::* Titration volume: 1 µL steps using a 10 µL syringe (2 mL O2k-Chamber).
::::* Titration volume: 1 µL steps using a 10 µL syringe (2 mL O2k-Chamber).
::::* Final concentration: 5 µM steps.
::::* Final concentration: 5 µM steps.
::::* Please consider that the final concentration needed in the O2k-Chamber may vary according to your cell type.
::::* Please consider that the final concentration needed in the O2k-Chamber may vary according to your cell type.
{{MitoPedia topics
|mitopedia topic=Medium
}}

Revision as of 15:58, 22 December 2020


high-resolution terminology - matching measurements at high-resolution


MitoKit-CII/Malonate-nv

Description

MitoKit-CII/Malonate-nv (diacetoxymethyl malonate) is a plasma membrane-permeable prodrug (permeable malonate; Mnanv) that diffuses across the plasma membrane. Cleavage of diacetoxymethyl residues is mediated by intracellular esterases, thus releasing malonate in the intracellular space. Abliva #: 01-161-s2

Abbreviation: Mnanv

Reference: MitoKit-CII, Ehinger 2016 Nat Commun


Application in HRR

Malonate-nv: 10 mg, 7.7 µL, 5.24 M, MW=248.1 g.mol-1
Preparation of 100 mM stock solution:
  1. Add 395.78 µL DMSO in 7.7 µL NV161.
  2. Divide into 40 µL portions.
  3. Store up to 6 months at -20 °C or up to 1 week at room temperature.
Preparation of 10 mM stock solution of Mnanv (from the 100 mM stock):
  1. Dilute 40 µL 100 mM stock solution with 360 µL DMSO.
  2. Divide into 100 µL portions.
  3. Store up to 6 months at -20 °C or up to 1 week at room temperature.
» O2k manual titrations
  • Event name: Mnanv
  • Titration volume: 1 µL steps using a 10 µL syringe (2 mL O2k-Chamber).
  • Final concentration: 5 µM steps.
  • Please consider that the final concentration needed in the O2k-Chamber may vary according to your cell type.

MitoPedia topics: Media for respirometry