MitoKit-CII/Malonate-nv

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MitoPedia

MitoKit-CII/Malonate-nv

Description

MitoKit-CII/Malonate-nv (diacetoxymethyl malonate) is a plasma membrane-permeable prodrug (permeable malonate; Mnanv) that diffuses across the plasma membrane. Cleavage of diacetoxymethyl residues is mediated by intracellular esterases, thus releasing malonate in the intracellular space. NeuroVive #: NV161

Abbreviation: Mnanv

Reference: MitoKit-CII, MitoKit-CII MitoPedia, Ehinger 2016 Nat Commun


Application in HRR

Malonate-nv: 10 mg, 7.7 µL, 5.24 M, MW=248.1 g.mol-1
Preparation of 100 mM stock solution:
  1. Add 395.78 µL DMSO in 7.7 µL NV161.
  2. Divide into 40 µL portions.
  3. Store up to 6 months at -20 °C or up to 1 week at room temperature.
Preparation of 10 mM stock solution of Mnanv (from the 100 mM stock):
  1. Dilute 40 µL 100 mM stock solution with 360 µL DMSO.
  2. Divide into 100 µL portions.
  3. Store up to 6 months at -20 °C or up to 1 week at room temperature.
» O2k manual titrations
  • Event name: Mnanv
  • Titration volume: 1 µL steps using a 10 µL syringe (2 mL O2k-Chamber).
  • Final concentration: 5 µM steps.
  • Please consider that the final concentration needed in the O2k-Chamber may vary according to your cell type.

MitoPedia topics: Media for respirometry