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Difference between revisions of "MitoKit-CII/Succinate-nv"

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{{MitoPedia
{{MitoPedia
|abbr=Snv
|abbr=Snv
|description='''MitoKit-CII/Succinate-nv''' (diacetoxymethyl succinate) is a plasma membrane-permeable prodrug (permeable succinate; Snv) that diffuses across the plasma membrane. Cleavage of diacetoxymethyl residues is mediated by intracellular esterases, thus releasing [[succinate]] in the intracellular space. NeuroVive #: NV118
|description='''MitoKit-CII/Succinate-nv''' (diacetoxymethyl succinate) is a plasma membrane-permeable prodrug (permeable succinate; Snv) that diffuses across the plasma membrane. Cleavage of diacetoxymethyl residues is mediated by intracellular esterases, thus releasing [[succinate]] in the intracellular space. Abliva #: 01-118-s4
|info=[[MitoKit-CII]], [[MitoKit-CII MitoPedia]], [[Ehinger 2016 Nat Commun]]
 
|info=[[MitoKit-CII]], [[Ehinger 2016 Nat Commun]]
 
}}
}}
{{MitoPedia topics
__TOC__
|mitopedia topic=Medium
}}
 
== Application in HRR ==
== Application in HRR ==
:::: '''Succinate-nv:''' 25 mg, MW=262.2 g.mol<sup>-1</sup>
::: '''Succinate-nv:''' 25 mg, MW=262.2 g.mol<sup>-1</sup>
:::: '''Preparation of 1 M stock solution:'''
:::: '''Preparation of 1 M stock solution:'''
::::# Dissolve 25 mg Snv in 95.3 µL DMSO.
::::# Dissolve 25 mg Snv in 95.3 µL DMSO.
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::::# Dilute 95.3 µL 1 M stock solution with 857.7 µL DMSO.
::::# Dilute 95.3 µL 1 M stock solution with 857.7 µL DMSO.
::::# Divide into 50 µL portions.   
::::# Divide into 50 µL portions.   
::::# Store at -20 °C.
::::# Store up to 4 months at -20 °C or up to 1 week at room temperature.


:::» '''O2k manual titrations'''  
:::» '''O2k manual titrations'''  
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::::* Final concentration: 50 µM steps.
::::* Final concentration: 50 µM steps.
::::* Please consider that the final concentration needed in the O2k-Chamber may vary according to your cell type.
::::* Please consider that the final concentration needed in the O2k-Chamber may vary according to your cell type.
{{MitoPedia topics
|mitopedia topic=Medium
}}

Revision as of 15:58, 22 December 2020


high-resolution terminology - matching measurements at high-resolution


MitoKit-CII/Succinate-nv

Description

MitoKit-CII/Succinate-nv (diacetoxymethyl succinate) is a plasma membrane-permeable prodrug (permeable succinate; Snv) that diffuses across the plasma membrane. Cleavage of diacetoxymethyl residues is mediated by intracellular esterases, thus releasing succinate in the intracellular space. Abliva #: 01-118-s4

Abbreviation: Snv

Reference: MitoKit-CII, Ehinger 2016 Nat Commun

Application in HRR

Succinate-nv: 25 mg, MW=262.2 g.mol-1
Preparation of 1 M stock solution:
  1. Dissolve 25 mg Snv in 95.3 µL DMSO.
Preparation of 100 mM stock solution of Snv (from the 1 M stock):
  1. Dilute 95.3 µL 1 M stock solution with 857.7 µL DMSO.
  2. Divide into 50 µL portions.
  3. Store up to 4 months at -20 °C or up to 1 week at room temperature.
» O2k manual titrations
  • Event name: Snv
  • Titration volume: 1 µL steps using a 10 µL syringe (2 mL O2k-Chamber).
  • Final concentration: 50 µM steps.
  • Please consider that the final concentration needed in the O2k-Chamber may vary according to your cell type.

MitoPedia topics: Media for respirometry