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| {{MitoPedia
| | #REDIRECT [[MitoKit-CII]] |
| |abbr=MitoKit-CII
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| |description='''Cell permeable prodrugs''', composed of [[MitoKit-CII/Succinate-nv]] and [[MitoKit-CII/Malonate-nv]], stimulates (Snv) or inhibits (Mnanv) mitochondrial respiration in CI-deficient human blood cells, fibroblasts and heart fibres, acting on Complex II of the electron transfer system.
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| |info=[[MitoKit-CII]], [[Ehinger 2016 Nat Commun]]
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| }}
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| {{MitoPedia topics
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| |mitopedia topic=Medium
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| }}
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| __TOC__
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| == Specific SUIT protocols ==
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| * [[SUIT-003_O2_ce_D060]] for living cells using [[MitoKit-CII/Succinate-nv]] and [[MitoKit-CII/Malonate-nv]].
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| * [[SUIT-003 O2 ce D050]] for living cells using [[MitoKit-CII/Succinate-nv]].
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| == Application in [[HRR]] ==
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| === Preparation ===
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| :::: Both compounds are dissolved in DMSO and stored in aliquots at -20 °C.
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| :::: We recommend the following dilutions for reaching the desired concentration in the experiments:
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| ::::* [[MitoKit-CII/Succinate-nv]]: 100 mM stock solution
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| ::::* [[MitoKit-CII/Malonate-nv]]: 10 mM stock solution
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| == SUITbrowser question: Permeable succinate/malonate ==
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| :::: The cell permeable prodrugs Succinate-nv and Malonate-nv can help to answer questions related to the S-pathway in [[living cells]]. Use the [https://suitbrowser.oroboros.at/ SUITbrowser] to find the best protocol to answer this and other research questions.
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