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MitoOx1
Description
Mitochondrial respiration medium, MitoOx1, used by the Budapest groups for respirometry und Amplex Red trials.
Abbreviation: MitoOx1
Reference: Komary 2010 Biochim Biophys Acta
MitoPedia topics: Media for respirometry
Application in HRR
MitoOx1: Mitochondrial Respiration Medium for measuring the H2O2 production
- Oxygen solubility factor of MitoOx1 at 30 °C and 37 °C = 0.95
Chemicals for MitoOx1
| Compound | Final conc. | M [g·mol-1] | Company, product code and storage |
|---|---|---|---|
| KCl | 125 mM | 74.55 | Sigma 31248, store at RT |
| HEPES free acid | 20 mM | 238.31 | Sigma H-7523 store at RT |
| KH2PO4 | 2 mM | 136.09 | Sigma P-5655, store at RT |
| MgCl2 | 2.86 mM | 203.30 | Scharlau MA0036, store at RT |
| EGTA free acid | 0.2 mM | 380.35 | Sigma E-4378, store at RT |
| BSA essentially fatty acid free | 0.025% | - | Sigma A-6003, store at 2 - 8 °C |
adjust pH to 7.0 (room temperature) with KOH
