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O2k-Open Support

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OROBOROS info


O2k-Manual and O2k-Protocols

O2k-technical support is based on and extends the guidelines provided with the O2k:

» O2k-Manual
» O2k-Protocols
» O2k-Publications: Topics

Standardized instrumental quality control tests are performed to evaluate and diagnose technical problems fast and cost-effectively.

» Polarographic oxygen sensors: calibration, accuracy and quality control SOP. »MiPNet06.03 POS-Calibration-SOP«
» Instrumental background correction, accuracy of oxygen flux and SOP. »MiPNet14.06 InstrumentalBackground«
» O2k-standard operating procedures: »O2k-SOP«

Open innovation and O2k-technical support

The OROBOROS-Team welcomes and values feedback from O2k-users and interested scientists. Technical and scientific questions, which are not yet addressed in the available guidelines or are difficult to trace on our website, will be relevant to both specialists and beginners in the field.


The OROBOROS-Team offers O2k-technical support based on open innovation:

Acceptance of O2k-technical support by the OROBOROS-Team implies the mutual agreement to publish the relevant corresponence or a summary of the correspondence addressing technical and scientific questions on O2k-technology and high-resolution respirometry, including names, institutional and Email addresses. Expansion of O2k-SOPs is particularly relevant for novel approaches in O2k-Fluorometry and O2k-Spectrophotometry, which are combined in the development of the NextGen-O2k. Open innovation extends the support given to individual users to the scientific community and provides feedback to improve the scope and documentation of the O2k-technology - in the spirit of Gentle Science.


O2k-technical service

Application problems

An application problem can be diagnosed on the basis of O2k-calibration and instrumental background tests performed in pure incubation medium in the absence of an experimental sample. Analysis of DatLab files recorded during instrumental tests is essential. If proper instrumental performance is obtained by resolving such application problems, any hardware problem is excluded.

Software problems

Communication between the O2k and DatLab running on a computer.

DatLab Error Message: Line has wrong length: Line has wrong length: This message appears when a signal line has not been transmitted properly from the O2k to DatLab. This is a problem only if the error message appears frequently, and may indicate that the specifications of the laptop are not adequate for proper DatLab performance.

Hardware problems

Diagnostic tests are available to identify a defective instrumental component. The two-chamber design of the O2k provides the option to exchange parts between the two chambers and thus localize a defective component: »Locating a problem

  1. Hardware problems that can be solved by user-service: The OroboPOS (polarographic oxygen sensor) requires user-service at intervals of more than 1 year, and SOPs are available to determine if a sensor service is required at an earlier or later date.
  2. Hardware problems that require replacement of a defective part.
  3. Some electronic or mechanical defects may be solved only by service of the O2k in the workshop of OROBOROS INSTRUMENTS, e.g. a defective Peltier unit.
DatLab Error Message: Block temperature: Average block temperature over past 10 min has not reached set temperature.


Cost estimation for repair

A cost estimation for repair of the O2k-Main unit due to an electronic/mechanical defect can only be given after inspection of the O2k by our workshop in Austria. Once a cost estimation has been provided, the repair is carried out after the customer’s consent. For shipment of the O2k, follow the detailed instructions.


O2k-technical support and open innovation pages

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