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Difference between revisions of "O2k-chamber"

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'''Trouble shooting procedure'''
'''Trouble shooting procedure'''
: If the problem is obviously related to the chamber proceed to solutions. If the problem manifests in a very low or zero raw signal, the possible contribution of chamber assembly to the problem is easily checked by doing the first step of  [[Locating_a_problem#Remove_components]]: if a very low or zero raw signal changes to the expected values (see [[Raw signal]] the problem is the chamber assembly.
: If the problem is obviously related to the chamber proceed to solutions. If the problem manifests in a very low or zero raw signal, the possible contribution of chamber assembly to the problem is easily checked by doing the first step of  [[Locating_a_problem#Remove_components]]: if a very low or zero raw signal changes to the expected values (see [[Raw signal]] the problem is the chamber assembly.


: Otherwise follow  [[Locating a problem]]
: Otherwise follow  [[Locating a problem]]
: If the problem is not located on the [[POS]] or the [[POS connector]] try solving the problem by reassembling the chamber before concluding that the [[main unit]] is defect.
: If the problem is not located on the [[POS]] or the [[POS connector]] try solving the problem by reassembling the chamber before concluding that the [[main unit]] is defective.


'''Solutions'''
'''Solutions'''
:* Disassemble the chamber by reversing the instructions in [[MiPNet19.18A O2k-Start]].
:* Disassemble the chamber by reversing the instructions in [[MiPNet19.18A O2k-Start]].
:* Check for visible damages on the [[glass chamber]], on the [[POS seal tip]] and all o-rings (on chamber holders or on stoppers). If a damage is visible replace the part or consult OROBOROS Instruments. If there is a deposit visible in the glass chamber see [[Cleaning the glass chamber]].
:* Check for visible damages on the [[glass chamber]], on the [[POS seal tip]] and all o-rings (on chamber holders or on stoppers). If damage is visible replace the part or consult OROBOROS Instruments. If there is a deposit visible in the glass chamber see [[Cleaning the glass chamber]].
:* Reassemble the chamber following exactly the description in [[MiPNet19.18A O2k-Start]]. Especially, use only small increments (not more  than successive quarter turns of the POS holder) when lowering the chamber.
:* Reassemble the chamber following exactly the description in [[MiPNet19.18A O2k-Start]]. In particular, use only small increments (not more  than successive quarter turns of the POS holder) when lowering the chamber.




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'''Solution'''
'''Solution'''
: The reason for this problem is very likely medium that has been spilled and acts now as a glue between glass chamber and copper block. Wash with plenty of warm water and try to remove the chamber while the O2k is switched on and heated up to 37 °C. You can try GENTLY to use the sensor holder (not the sensor connector) to try to push up the glass chamber (like you do during [[chamber assembly]]) - while the O2k is warm and with plenty of warm water.  Since the sensors are removed you also can use e.g. 40° (or a little bit more) for the washing / removing attempts.
: The reason for this problem is very likely to be that medium has been spilled and is now acting now as an adhesive between glass chamber and copper block. Wash with plenty of warm water and try to remove the chamber while the O2k is switched on and heated up to 37 °C. You can try GENTLY to use the sensor holder (not the sensor connector) to try to push the glass chamber upwards (like you do during [[chamber assembly]]) - while the O2k is warm and with plenty of warm water.  With the sensors removed you can also try using a higher temperature, e.g. 40° C (or even slightly higher) for the washing / removing attempts.





Revision as of 14:40, 19 May 2015

                



O2k-chamber

O2k-Catalogue

Description O2k-Chamber: 16 mm inner diameter, Duran glass polished, with standard operation volume (V) of 2 cm3 (2 ml).

Two units of this item are standard components of the O2k-Assembly Kit (O2k-Core). The optical properties of Duran allow application in the O2k-Fluorometer (Duran optical properties).

Product ID 23100-01
Type O2k, O2k-Assembly Kit
Link MiPNet09.01 O2k-ParadigmShift
Image
Glass chamber.jpg

A spare (third) O2k-Chamber may be added to the items ordered with the O2k-System. For dispatch of the O2k, the O2k-Chambers are shipped in O2k-Service Box.


O2k-technical support

Cleaning the glass chamber


Template NextGen-O2k.jpg


MitoPedia O2k and high-resolution respirometry: O2k-Open Support 



Standard operating procedure - cleaning and storage of the O2k-Chamber: >> MiPNet19.03 O2k-cleaning and ISS


Chamber assembly


Template NextGen-O2k.jpg


MitoPedia O2k and high-resolution respirometry: O2k-Open Support 



The assembly of the chamber is described in MiPNet19.18A_O2k-Start.

Possible problems

  • Chamber leaking liquids (medium is leaking out of the chamber)
  • Oxygen leak of the chamber (strongly negative intercept in the instrumental background test
  • Unstable O2 signal
  • Unexpected high or low O2 signal

Trouble shooting procedure

If the problem is obviously related to the chamber proceed to solutions. If the problem manifests in a very low or zero raw signal, the possible contribution of chamber assembly to the problem is easily checked by doing the first step of Locating_a_problem#Remove_components: if a very low or zero raw signal changes to the expected values (see Raw signal the problem is the chamber assembly.
Otherwise follow Locating a problem
If the problem is not located on the POS or the POS connector try solving the problem by reassembling the chamber before concluding that the main unit is defective.

Solutions

  • Disassemble the chamber by reversing the instructions in MiPNet19.18A O2k-Start.
  • Check for visible damages on the glass chamber, on the POS seal tip and all o-rings (on chamber holders or on stoppers). If damage is visible replace the part or consult OROBOROS Instruments. If there is a deposit visible in the glass chamber see Cleaning the glass chamber.
  • Reassemble the chamber following exactly the description in MiPNet19.18A O2k-Start. In particular, use only small increments (not more than successive quarter turns of the POS holder) when lowering the chamber.


Chamber volume calibration


Template NextGen-O2k.jpg


MitoPedia O2k and high-resolution respirometry: O2k-Open Support 



The volume calibration of the chamber is described in MiPNet19.18A_O2k-Start.
Old model stopper: if you use blue Titanium stoppers: check the manual delivered with your O2k or contact OROBOROS INSTRUMENTS ([email protected]) for instructions.


Glass chamber stuck in the O2k


Template NextGen-O2k.jpg


MitoPedia O2k and high-resolution respirometry: O2k-Open Support 



Problem

After removing OroboPOS-Connector, OroboPOS-Holder, and O2k-Chamber Holder from the O2k , the O2k-Chamber cannot be removed. It remains stuck even after washing with water.

Solution

The reason for this problem is very likely to be that medium has been spilled and is now acting now as an adhesive between glass chamber and copper block. Wash with plenty of warm water and try to remove the chamber while the O2k is switched on and heated up to 37 °C. You can try GENTLY to use the sensor holder (not the sensor connector) to try to push the glass chamber upwards (like you do during chamber assembly) - while the O2k is warm and with plenty of warm water. With the sensors removed you can also try using a higher temperature, e.g. 40° C (or even slightly higher) for the washing / removing attempts.