Difference between revisions of "Oliveira Pinto 2013 Abstract IOC75"
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== Affiliations and author contributions == | == Affiliations and author contributions == | ||
Institute of Medical Biochemistry, Federal University of Rio de Janeiro | |||
Revision as of 15:54, 8 March 2013
| Oliveira Pinto M (2013) Studies on the energy and redox metabolism of the blood fluke Schistosoma mansoni. Mitochondr Physiol Network 18.03. |
Link: IOC75 Open Access
Oliveira Pinto M, Oliveira MF (2013)
Event: IOC75
The blood fluke Schistosoma mansoni is an intravascular parasite which causes human schistosomiasis. Within the human host, the adult forms of S. mansoni depend on the ingestion of large amounts of blood to supply their energy demands. Furthermore, evidence indicates that important metabolic changes occur in worms in several stages of their life cycle, so that the cercariae present essentially an oxidative metabolism, while the adult forms living within the vertebrate blood vessels have a more fermentative metabolism. Furthermore, the activity and expression of antioxidant enzymes increases during development of the worm in the vertebrate host which is parallel with the ingestion of blood. Thus, we aim to investigate the redox and energy metabolism in adult forms of S. mansoni. We observed that the oxygen consumption associated with the Electron Transport System (ETS) is significantly lower in females than in males. This phenomenon occurs independently of the type of substrate used by worms. Glucose uptake was higher in females than in males suggesting increased dependence on glucose metabolism in females. Evaluation of ETS complexes activities showed that females have higher complex I-III and lower complex IV compared to males. Hydrogen peroxide production was significantly higher in whole females than in males. Pro-oxidant challenge with menadione shoed that females are significant more resistant than males. We therefore conclude that there is a difference in energy metabolism and redox between adult female and adult male worms, which may be relevant to allow these organisms to the blood feeding habit.
• Keywords: Schistosoma mansoni, metabolism, redox resistance
• O2k-Network Lab: BR_Rio de Janeiro_Oliveira MF
Labels:
Stress:RONS; Oxidative Stress"RONS; Oxidative Stress" is not in the list (Cell death, Cryopreservation, Ischemia-reperfusion, Permeability transition, Oxidative stress;RONS, Temperature, Hypoxia, Mitochondrial disease) of allowed values for the "Stress" property. Organism: Other Non-Mammal"Other Non-Mammal" is not in the list (Human, Pig, Mouse, Rat, Guinea pig, Bovines, Horse, Dog, Rabbit, Cat, ...) of allowed values for the "Mammal and model" property.
Preparation: Intact Organism"Intact Organism" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
Regulation: Redox State"Redox State" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property. Coupling state: OXPHOS
HRR: Oxygraph-2k
Affiliations and author contributions
Institute of Medical Biochemistry, Federal University of Rio de Janeiro
