Difference between revisions of "Pyruvate"
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{{MitoPedia | {{MitoPedia | ||
|abbr=P | |abbr=P | ||
|description= | |description=Pyruvate as a product of glycolysis is an anion that enters mitochondrial matrix via a specific low Km transporter [[pyruvate carrier]]. | ||
In the mt matrix the oxidative decarboxylation of '''pyruvate''' is catalyzed by [[pyruvate dehydrogenase complex]] and yields acetyl-CoA. | |||
|info=[[MiPNet11.04]], [[MiPNet09.12]] | |info=[[MiPNet11.04]], [[MiPNet09.12]] | ||
|type=Substrate ETS | |type=Substrate ETS | ||
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'''P: Pyruvate''' (Pyruvic acid, sodium salt, C<sub>3</sub>H<sub>3</sub>O<sub>3</sub>Na); Sigma P 2256, 25 g, store at R.T.: FW = 110.0) | '''P: Pyruvate''' (Pyruvic acid, sodium salt, C<sub>3</sub>H<sub>3</sub>O<sub>3</sub>Na); Sigma P 2256, 25 g, store at R.T.: FW = 110.0) | ||
'''Preparation of 2 M stock solution''' (dissolved in H<sub>2</sub>O): | '''Preparation of 2 M stock solution''' (dissolved in H<sub>2</sub>O): | ||
| Line 25: | Line 22: | ||
::1) weigh 44 mg of L-Glutamic acid directyl into 1 ml Eppendorf tube | ::1) weigh 44 mg of L-Glutamic acid directyl into 1 ml Eppendorf tube | ||
::2) add 0.2 ml H<sub>2</sub>O | ::2) add 0.2 ml H<sub>2</sub>O | ||
::3) adjust pH to 7 | |||
'''Oxygraph-2k Manual Titrations:''' [[MiPNet09.12]] | '''Oxygraph-2k Manual Titrations:''' [[MiPNet09.12]] | ||
Revision as of 10:31, 3 December 2010
- high-resolution terminology - matching measurements at high-resolution
Pyruvate
Description
Pyruvate as a product of glycolysis is an anion that enters mitochondrial matrix via a specific low Km transporter pyruvate carrier. In the mt matrix the oxidative decarboxylation of pyruvate is catalyzed by pyruvate dehydrogenase complex and yields acetyl-CoA.
Abbreviation: P
Reference: MiPNet11.04, MiPNet09.12
Labels:
Enzyme: TCA Cycle and Matrix Dehydrogenases"TCA Cycle and Matrix Dehydrogenases" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property. Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Substrate; Glucose; TCA Cycle"Substrate; Glucose; TCA Cycle" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
Application in HRR
P: Pyruvate (Pyruvic acid, sodium salt, C3H3O3Na); Sigma P 2256, 25 g, store at R.T.: FW = 110.0)
Preparation of 2 M stock solution (dissolved in H2O):
- !!Prepare fresh every day!!
- 1) weigh 44 mg of L-Glutamic acid directyl into 1 ml Eppendorf tube
- 2) add 0.2 ml H2O
- 3) adjust pH to 7
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 5 µl using a 25 µl syringe
- Final conc. in 2 ml O2k-chamber: 5 mM
