Difference between revisions of "Pyruvate"
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|mitopedia topic=Substrate and metabolite | |mitopedia topic=Substrate and metabolite | ||
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== Application in [[ | == Application in [[HRFR]] == | ||
::: '''P: Pyruvate''' (pyruvic acid, sodium salt, C<sub>3</sub>H<sub>3</sub>O<sub>3</sub>Na); Sigma P 2256, 25 g, store at 4 °C; FW = 110.0 | ::: '''P: Pyruvate''' (pyruvic acid, sodium salt, C<sub>3</sub>H<sub>3</sub>O<sub>3</sub>Na); Sigma P 2256, 25 g, store at 4 °C; FW = 110.0 | ||
::: '''Preparation of 2 M stock solution''' (dissolved in H<sub>2</sub>O) | ::: '''Preparation of 2 M stock solution''' (dissolved in H<sub>2</sub>O) | ||
::::# Weigh 44 mg of pyruvic acid directyl into a 0 | ::::# Prepare fresh everyday. | ||
::::# Weigh 44 mg of pyruvic acid directyl into a 0.5 ml Eppendorf tube. | |||
::::# Add 0.2 ml H<sub>2</sub>O. | ::::# Add 0.2 ml H<sub>2</sub>O. | ||
::::# Adjust pH to 7.0. | ::::# Adjust pH to 7.0. | ||
Revision as of 11:22, 22 August 2017
- high-resolution terminology - matching measurements at high-resolution
Pyruvate
Description
Pyruvic acid, C3H4O3, is an alpha-keto monocarboxylic acid which occurs under physiological conditions mainly as the anion pyruvate-, P, with pKa = 2.5. Pyruvate is formed in glycolysis from phosphoenolpyruvate. In the cytosol, pyruvate is a substrate of lactate dehydrogenase. Pyruvate enters the mitochondrial matrix via a specific low Km' H+/monocarboxylate cotransporter known as the pyruvate carrier. Similarly, the plasma membrane of many cell types has H+/monocarboxylate cotransporter activity and pyruvate can thus be added as a substrate to intact cells. In the mt-matrix the oxidative decarboxylation of pyruvate is catalyzed by pyruvate dehydrogenase and yields acetyl-CoA. Pyruvate competitively reverses the inhibition of cytochrome c oxidase by cyanide. Pyruvate is an antioxidant reacting with hydrogen peroxide.
Abbreviation: P
Reference: Gnaiger 2014 MitoPathways, MiPNet09.12 O2k-Titrations
MitoPedia topics:
Substrate and metabolite
Application in HRFR
- P: Pyruvate (pyruvic acid, sodium salt, C3H3O3Na); Sigma P 2256, 25 g, store at 4 °C; FW = 110.0
- Preparation of 2 M stock solution (dissolved in H2O)
- Prepare fresh everyday.
- Weigh 44 mg of pyruvic acid directyl into a 0.5 ml Eppendorf tube.
- Add 0.2 ml H2O.
- Adjust pH to 7.0.
- O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume: 5 µl using a 25 µl syringe (2 ml O2k-chamber).
- Final concentration: 5 mM.
