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Pyruvate

From Bioblast


high-resolution terminology - matching measurements at high-resolution


Pyruvate

Description

Pyruvate as a product of glycolysis is an anion that enters mitochondrial matrix via a specific low Km transporter pyruvate carrier. In the mt matrix the oxidative decarboxylation of pyruvate is catalyzed by pyruvate dehydrogenase complex and yields acetyl-CoA.

Abbreviation: P

Reference: MiPNet11.04, MiPNet09.12


MitoPedia topics: Substrate and metabolite 

Application in HRR

P: Pyruvate (Pyruvic acid, sodium salt, C3H3O3Na); Sigma P 2256, 25 g, store at R.T.); FW = 110.0

Preparation of 2 M stock solution (dissolved in H2O):

!!Prepare fresh every day!!
1) weigh 44 mg of L-Glutamic acid directyl into 1 ml Eppendorf tube
2) add 0.2 ml H2O
3) adjust pH to 7


Oxygraph-2k Manual Titrations: MiPNet09.12

  • Titration volume: 5 Β΅l using a 25 Β΅l syringe
  • Final conc. in 2 ml O2k-chamber: 5 mM