Difference between revisions of "Startup O2k-Respirometer"

Revision as of 12:22, 3 May 2017

Startup O2k-Respirometer

Description	OROBOROS O2k-Core - the experimental system complete for basic high-resolution respirometry (HRR). The O2k-Core includes the O2k-Main Unit with stainless steel housing, O2k-Assembly Kit, two OroboPOS (polarographic oxygen sensors) and OroboPOS-Service Kit, DatLab software, the ISS-Integrated Suction System and the O2k-Titration Set. The O2k-Core supports all add-on O2k-Modules of the O2k, and is a component of the O2k-Fluorometer. On-line display of oxygen flux (rate of respiration) is provided in addition to the conventional 'oxygraphic' plot of oxygen concentration over time. Highest signal stability minimizes the required amounts of biological sample, and provides the basis for resolution in the extreme low-oxygen range. Peltier temperature control provides a thermal stability at ±0.002 °C in the range of 4 °C to 47 °C at typical constant room temperature. Electronically controlled PVDF or PEEK stirrers are integrated in the two-chamber design of the O2k, and a barometric pressure transducer enables automatic oxygen calibrations implemented in the DatLab software. The O2k is a sole source apparatus with no other instruments meeting its specifications.
Product ID	10000-02
Type	O2k, O2k-Module, O2k-Fluorometer, Catalogue
Link	O2k, Gnaiger_2008_POS
Image

The O2k-Core does not include, but fully supports and is required for the add-on Modules of the O2k, such as the TIP2k, O2k-Fluo LED2-Module, O2k-TPP+ ISE-Module, O2k-pH ISE-Module, O2k-NO Amp-Module.

A PC is required (not included), connecting DatLab to the O2k via a USB port. For PC requirements, see here.

» Keywords

» DatLab

» DatLab PC requirements

» ISS-Integrated Suction System

» O2k

» O2k-Assembly Kit

» O2k-Fluorometer

» O2k-Fluo LED2-Module

» O2k high-resolution respirometry

» O2k-TPP+ ISE-Module

» OroboPOS

» OroboPOS-Service Kit

» Oxygraph specifications

» TIP2k

The O2k-Core consists of

O2k-Core manual and selected protocols

» Contents: O2k-Core Manual DatLab 6.pdf

Chapter	View	Section	Last update
Gnaiger 2008 POS		Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial dysfunction in drug-induced toxicity (Dykens JA, Will Y, eds) John Wiley & Sons, Inc, Hoboken, NJ:327-52.	2008
Gnaiger 2020 BEC MitoPathways	published online 2020-12-30	Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5^th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002	2020
MiPNet04.05 Titration-Injection		From step titration to ramp injection: Uncoupling by FCCP with TIP.	1999-01
MiPNet07.08 User information	» Versions	O2k-Manual: user information. PLEASE STUDY THIS MANUAL.	2017-11-03
MiPNet08.09 CellRespiration	»Versions	High-resolution respirometry and coupling-control protocol with living cells: ROUTINE, LEAK, ET-pathway, ROX.	2016-08-11
MiPNet08.12 IOC22		NO effect on mitochondrial oxygen kinetics at low oxygen. O2k workshop Report.	2011-12-11
MiPNet12.01 Suppl T-issue	»Versions	MitoPathways at the Q-junction: mouse skeletal muscle fibers.	2018-11-18
MiPNet12.11 MitoRespiration	»Versions	Flux control ratios in isolated mitochondria. OXPHOS capacity and respiratory control.	2014-02-20
MiPNet17.03 Shredder vs Fibers	»Versions	Mitochondrial respiration in permeabilized fibers versus homogenate from fish liver and heart. An application study with the PBI-Shredder.	2015-06-18
MiPNet19.18 O2k-Series G Core manual	»Versions	O2k-Core manual contents.	2016-08-08
MiPNet19.18B POS-service	»Versions	Service of the polarographic oxygen sensor OroboPOS.	2021-06-23
MiPNet19.18C DatLab 6: Guide	»Versions	DatLab-guide through the menus.	2016-08-24
MiPNet19.18D O2k-Series G and DatLab 6: Calibration	»Versions	O2k-calibration by DatLab 6.	2016-08-24
MiPNet19.18E O2 flux analysis - DatLab 6	»Versions	Oxygen flux analysis: DatLab real-time.	2016-08-08
MiPNet21.14 Reference sample HRR	»Versions	Development of a reference sample for HRR.	2016-10-19
MiPNet21.16 DatLab 7 Innovations		DatLab 7: innovations.	2019-09-23
MiPNet22.16 DatLab 7 Innovations: DL-Protocols	» Versions	DatLab 7.4 Innovations: DL-Protocols	2019-09-13
MiPNet23.12 DL-Protocols help	» Versions	DatLab Protocols: Help DatLab 7.	2019-09-13
MiPNet24.06 Oxygen flux analysis - DatLab 7.4	» Versions	Oxygen flux analysis with DatLab 7.4.	2020-04-16
MiPNet24.08 Safranin Analysis Template	» Versions	Excel template for safranin data analysis.	2020-05-13
MiPNet24.09 Data analysis of mt-membrane potential	» Versions	Data analysis of mt-membrane potential.	2021-09-16
MiPNet25.14 TPP Analysis Template	» [https:.pdf Versions]	Excel template for TPP data analysis.	2020-##-##
MiPNet26.06 DatLab 7: Guide	»Versions	DatLab-guide through the menus.	2021-06-02
MiPNet28.10 SmartPOS-service	» Versions	SmartPOS: Service	2023-09-29
Pesta 2012 Methods Mol Biol	PMID: 22057559	Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibers from small biopsies of human muscle. Methods Mol Biol 810:25-58. https://doi.org/10.1007/978-1-61779-382-0_3	2012

MitoPedia O2k and high-resolution respirometry: O2k hardware

@@ Line 30: / Line 30: @@
 ::::» [[O2k-NO Amp-Module]]
 ::::» [[O2k-pH ISE-Module]]
+::::» [[O2k-Titration Set]]
+::::» [[O2k-TPP+ ISE-Module]]
 ::::» [[OroboPOS]]
 ::::» [[OroboPOS-Service Kit]]
+::::» [[MiPNet06.05 Specifications | Oxygraph specifications]]
 ::::» [[Titration-Injection microPump|TIP2k]]
-::::» [[O2k-Titration Set]]
-::::» [[O2k-TPP+ ISE-Module]]
-::::» [[MiPNet06.05 Specifications | Oxygraph specifications]]
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