Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Difference between revisions of "Talk:Oroboros FAQ"

From Bioblast
Line 7: Line 7:


__TOC__
__TOC__
== Applications and samples ==
<br/>
<div class="toccolours mw-collapsible mw-collapsed" style="background:#F8E0E0">
<span style="font-size:105%; color:#424242">'''Which are the most common applications of the [[Oxygraph-2k|OROBOROS Oxygraph-2k]] (O2k)?'''</span>
<div class="mw-collapsible-content">
The O2k is used in basic and applied research to study the respiration of tissues, cells and mitochondria. Fairly simple to highly complex, comprehensive experimental protocols can be applied to assess either basal mitochondrial characteristics or obtain diagnostic insight into respiratory pathways. Topically, studies using the O2k range from [[O2k-Publications: Comparative MiP;environmental MiP|comparative physiological]] investigations addressing evolutionary and environmental aspects of mitochondrial metabolism over the energetics related to exercise physiology,Β  [[O2k-Publications: Exercise physiology;nutrition;life style|nutrition and life style]], to aspects of clinical interest such as the impact of [[O2k-Publications: Mitochondrial medicine|metabolic diseases]], [[O2k-Publications: Cancer|cancer]], or [[O2k-Publications: Pharmacology;toxicology|pharmacological interventions]].
<br/><br/><br/>
::: '''The most prominent applications of the O2k'''
::::* <span style="color:#424242">'''Studies on exercise physiology;nutrition;life style'''</span>
::::::Β» {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] [[MiP area::Exercise physiology;nutrition;life style]] |format=count}} [[O2k-Publications: Exercise physiology;nutrition;life style|O2k-Publications in the MiPMap]]
::::* <span style="color:#424242">'''Diagnosis of acquired and genetic mitochondrial diseases'''</span>
::::::Β» {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] [[Diseases::Inherited]] |format=count}} [[O2k-Publications: Mitochondrial disease|O2k-Publications in the MiPMap]]
::::* <span style="color:#424242">'''Diagnosis of myopathies and neuromuscular pathologies'''</span>
::::::Β» {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] [[Diseases::Myopathy]] |format=count}} [[O2k-Publications: Myopathy|O2k-Publications in the MiPMap]]
::::* <span style="color:#424242">'''Studies on cell function and cell death - apoptosis and necrosis'''</span>
::::::Β» {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] [[Injury andΒ  adaptation::Cell death]] |format=count}} [[O2k-Publications: Cell death|O2k-Publications in the MiPMap]]
::::* <span style="color:#424242">'''Measurement of oxidative stress'''</span>
::::::Β» {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] [[Injury andΒ  adaptation::Oxidative stress;RONS]] |format=count}} [[O2k-Publications: RONS; Oxidative stress|O2k-Publications in the MiPMap]]
::::* <span style="color:#424242">'''Studies on ischemia-reperfusion injury'''</span>
::::::Β» {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] [[Injury andΒ  adaptation::Ischemia-reperfusion]] |format=count}} [[O2k-Publications: Ischemia-reperfusion|O2k-Publications in the MiPMap]]
::::* <span style="color:#424242">'''Studies on aging and senescence'''</span>
::::::Β» {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] [[Diseases::Aging;senescence]] |format=count}} [[O2k-Publications: Aging; senescence|O2k-Publications in the MiPMap]]
::::* <span style="color:#424242">'''Pharmacological tests'''</span>
::::::Β» {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] [[MiP area::Pharmacology;toxicology]] |format=count}} [[O2k-Publications: Pharmacology;toxicology|O2k-Publications in the MiPMap]]
::::* <span style="color:#424242">'''Studies on Diabetes 2'''</span>
::::::Β» {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] [[Diseases::Diabetes]] |format=count}} [[O2k-Publications: Diabetes 2|O2k-Publications in the MiPMap]]
::::* <span style="color:#424242">'''For more applications, browse through the {{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]] |format=count}} [[O2k-Publications:_Topics|O2k-Publications in the MiPMap]].'''</span></div>
</div>
<div class="toccolours mw-collapsible mw-collapsed">
<span style="font-size:105%; color:#424242"> '''What is high-resolution respirometry and what is so special about it?''' </span>
<div class="mw-collapsible-content">
High-resolution respirometry is the method of choice when aiming at investigating mitochondrial function either in intact cells or various types of mitochondrial preparation. Combining instrumental accuracy and reliability with versatility of applicable protocols - allowing practically unlimited addition and combination of substrates, inhibitors and uncouplers - mitochondrial respiratory pathways may be analyzed in detail to evaluate even minor alterations in respiratory pathway control and/or capacity.
The O2k is a sole source apparatus, with no other available instrument meeting its specifications for high-resolution respirometry.
[[O2k high-resolution respirometry|'''High-resolution respirometry''']] '''(HRR)''' is based on the O2k, combining chamber design, application of oxygen-tight materials, electrochemical and optical sensors, Peltier-temperature control and software features [[DatLab|(DatLab)]] to obtain the unique sensitive and quantitative resolution of oxygen concentration and oxygen flux, with both, a closed- and open-chamber mode of operation.
</div>
</div>
<div class="toccolours mw-collapsible mw-collapsed" style="background:#F8E0E0">
<span style="font-size:105%; color:#424242">'''Is the O2k easy to use in standard applications of mitochondrial respiration?'''</span>
<div class="mw-collapsible-content"> The O2k is a robust instrument, ready to be used for standard applications, providing high reliability, stability of the oxygen sensor over several months, and full software support. See: [[DatLab|DatLab]]</div>
</div>
<div class="toccolours mw-collapsible mw-collapsed">
<span style="font-size:105%; color:#424242"> '''Most 'oxygraphs' are designed for analyzing isolated mitochondria. Which types of samples can be analyzed with the O2k?'''</span>
<div class="mw-collapsible-content"> The O2k can be routinely applied for cultured cells, [[Intact cells|intact cells]] and tissue biopsies, [[Permeabilized tissue or cells|permeabilized cells]] and tissue biopsies, microbial cultures, oxygen-consuming enzymes, antioxidant systems, and, of course, [[Isolated mitochondria|isolated mitochondria]]. See: [[Respirometry|Respirometry]] and [[O2k-Publications: Topics|O2k-Publications]].</div>
</div>
<div class="toccolours mw-collapsible mw-collapsed" style="background:#F8E0E0">
<span style="font-size:105%; color:#424242"> '''Can the O2k be applied for the measurement of oxygen consumption in delicate mammalian cells, when limited numbers of cells are available?'''</span>
<div class="mw-collapsible-content"> Compared to other instruments, the O2k requires five to ten times lower cell numbers for routine measurements of cell respiration, making it highly cost-, personnel- and time-efficient.</div>
</div>
<div class="toccolours mw-collapsible mw-collapsed">
<span style="font-size:105%; color:#424242">'''Can the O2k be applied for measuring human biopsies?'''</span>
<div class="mw-collapsible-content"> When [[Permeabilized tissue or cells|permeabilized]] biopsy preparations are utilized, the O2k provides the unique potential to reduce sample size to as little as 1 mg fresh weight per experimental run, supporting application of complex titration protocols.</div>
</div>
<div class="toccolours mw-collapsible mw-collapsed" style="background:#F8E0E0">
<span style="font-size:105%; color:#424242">'''Can the O2k be applied to measure tissue oxygen concentration?'''</span>
<div class="mw-collapsible-content"> No. The O2k is a [[O2k-Chamber|'chamber']] approach, for application with tissue biopsies, cultured cells or [[Isolated_mitochondria|isolated mitochondria]]. Whereas HRR cannot be applied for measurement of tissue O<sub>2</sub> concentration, it is the method of choice for analysis of tissue O<sub>2</sub> consumption and functional diagnosis of mitochondrial injuries.</div>
</div>
<div class="toccolours mw-collapsible mw-collapsed">
<span style="font-size:105%; color:#424242">'''Can the O2k be applied for measurement of oxygen consumption in [[Intact cells|adherent cells]]?'''</span>
<div class="mw-collapsible-content"> Attached cells may be grown on disks before being introduced into the [[O2k-Chamber|cylindrical chamber]] of the O2k. Alternatively, cells attached to beads can be used. With this method, however, gentle stirring, with the use of specific small [[Stirrer-Bar\white PVDF\15x6 mm|stirring bars]], is advisable to avoid crashing of the beads. Routinely, we apply the O2k on adherent cells after transfering them into suspension. For small cells, such as fibroblasts, at least 200,000 cells per chamber are required.</div>
</div>
<div class="toccolours mw-collapsible mw-collapsed" style="background:#F8E0E0">
<span style="font-size:105%; color:#424242">'''How many parameters can be measured simultaneously?'''</span>
<div class="mw-collapsible-content"> The O2k is designed to measure respiration plus one additional parameter at a time. Typical applications of [[O2k-MultiSensor|O2k-MultiSensor respirometry]] are the combined measurement of respiration and H<sub>2</sub>O<sub>2</sub> production using the [[O2k-Fluo LED2-Module]] or respiration and mitochondrial membrane potential with either the [[O2k-Fluo LED2-Module]] or the [[O2k-TPP+ ISE-Module|O2k-TPP+ ISE-Module]].</div>
</div>
== Software compatibility ==
<br/>
<div class="toccolours mw-collapsible mw-collapsed" style="background:#F5F6CE">
<span style="font-size:105%; color:#424242">'''Does [http://www.bioblast.at/index.php/DatLab DatLab] software run on MAC devices?'''</span>
<div class="mw-collapsible-content"> No. The [http://www.bioblast.at/index.php/DatLab DatLab] software is designed for Windows operating systems.</div>
</div>
== O2k in comparison with other devices ==
<br/>
<div class="toccolours mw-collapsible mw-collapsed" style="background:#E0E6F8">
<span style="font-size:105%; color:#424242">'''Why buy an O2k and not a different oxygraph?'''</span>
<div class="mw-collapsible-content"> Β» [http://www.bioblast.at/index.php/MiPNet14.10_O2k-Top_10 Top 10 reasons for the O2k]
Β» [http://www.bioblast.at/index.php/Talk:O2k_versus_multiwell_respirometer O2k versus multiwell respirometer] </div>
</div>
== How and where to buy ==
<br/>
<div class="toccolours mw-collapsible mw-collapsed" style="background:#F8E0EC">
<span style="font-size:105%; color:#424242">'''Do you have any distributors in my country?</span>
<div class="mw-collapsible-content">
With the exception of the countries listed below, '''OROBOROS INSTRUMENTS''' has a direct-sales approach. This means, we generally sell our products directly to the end user. If required by the end user, it is possible to cooperate with a distributor. We cooperate with our distributors in the following countries:
::* China: [http://www.bioblast.at/index.php/CN_Beijing_Zenda CN Beijing Zenda].
::* Taiwan: [http://www.bioblast.at/index.php/TW_Taoyuan_Sunpoint TW Taoyuan Sunpoint].
::* Republic of Korea: [http://www.bioblast.at/index.php/KR_Seoul_Mymed KR Seoul Mymed].
::* Australia: [http://www.bioblast.at/index.php/AU_Adelaide_AIMedical AU Adelaide AIMedical].
::* New Zealand: [http://www.bioblast.at/index.php/NZ_Auckland_GBC_BioMed NZ Auckland GBC BioMed].
::* Japan: [http://www.bioblast.at/index.php/JP_Tokyo_Sanyo JP Tokyo Sanyo]. We are also open to requests for specific distributors as well as direct sales.
::* Malaysia: [http://www.bioblast.at/index.php/MY_Puchong_Selangor_Saujana_Saintifik MY Puchong Selangor Saujana Saintifik]. We are also open to requests for specific distributors as well as direct sales.</div>
</div>
<div class="toccolours mw-collapsible mw-collapsed">
<span style="font-size:105%; color:#424242">'''How do I obtain price information on the O2k?'''</span>
<div class="mw-collapsible-content"> Further information under [http://www.bioblast.at/index.php/Purchase#Prices Purchase].</div>
</div>
<div class="toccolours mw-collapsible mw-collapsed" style="background:#F8E0EC">
<span style="font-size:105%; color:#424242">'''Whom do I contact to buy an O2k'''</span>
<div class="mw-collapsible-content"> In order to request a quote or order an O2k or spare parts, please contact our Admin Team: [mailto:[email protected] [email protected]] </div>
</div>
== Delivery content, warranty and support ==
<br/>
<div class="toccolours mw-collapsible mw-collapsed" style="background:#E6F8E0">
<span style="font-size:105%; color:#424242">'''How long from order till delivery?</span>
<div class="mw-collapsible-content">
The O2k is produced on demand, i.e. upon receipt of your purchase order. Therefore, the current lead time for delivery is ten (10) weeks. Depending on the workload of our workshop, this time span may be shortened.</div>
</div>
<div class="toccolours mw-collapsible mw-collapsed">
<span style="font-size:105%; color:#424242">'''Does the purchase include installation, setup and training?'''</span>
<div class="mw-collapsible-content">
* '''Installation and setup'''
On-site installation and setup is not included in the purchase of an [http://www.bioblast.at/index.php/O2k-Core O2k-Core]. Setup and installation are usually performed by the customers themselves with the help of our detailed and user-friendly [http://www.bioblast.at/index.php/O2k-Manual O2k-Manual] which is sent out with the O2k. In addition, the manual can also be easily down-loaded form our website [http://www.bioblast.at/index.php/O2k-Manual (O2k-Manual)]. There is also a section for [http://www.bioblast.at/index.php/O2k-technical_service technical service]. Our technical specialists, based here in Innsbruck, can also assist in helping to locate and solve any technical problems, should these arise. Based on our experience and feedback received from customers all over the world, we are confident that you will easily master the set-up and installation.
<br/>
* '''Training'''
The purchase of an O2k includes a waived registration fee for our [http://www.bioblast.at/index.php/O2k-Workshop '''O2k-Workshop'''], which takes place at regular intervals all over the world. For more information on our upcoming O2k-Workshops (IOC), please take a look at [http://www.bioblast.at/index.php/OROBOROS_Events '''OROBOROS Events'''] or send an Email to: [mailto:[email protected] [email protected]].</div>
</div>
<div class="toccolours mw-collapsible mw-collapsed" style="background:#E6F8E0">
<span style="font-size:105%; color:#424242">'''Does the [http://www.bioblast.at/index.php/O2k-Core O2k-Core] include a PC or do we have to purchase it separately?'''</span>
<div class="mw-collapsible-content">
No, the purchase of an O2k does not include a PC. However, a laptop or a PC [http://www.bioblast.at/index.php/DatLab#PC_requirements (PC Requirements)] is required to connect the O2k and run the required software (DatLab).</div>
</div>
<div class="toccolours mw-collapsible mw-collapsed">
<span style="font-size:105%; color:#424242">'''Is it possible to extend the warranty period?'''</span>
<div class="mw-collapsible-content">
Yes, it is possible to extend the warranty period by a total of three years. For more details, please contact our office: [mailto:[email protected] [email protected]]</div>
</div>
<div class="toccolours mw-collapsible mw-collapsed" style="background:#E6F8E0">
<span style="font-size:105%; color:#424242">'''Are taxes and duty fees included in the price?'''</span>
<div class="mw-collapsible-content">
No, our offer does not include any taxes or any applicable duty fees. Please consider this when budgeting the purchase.</div>
</div>
<div class="toccolours mw-collapsible mw-collapsed">
<span style="font-size:105%; color:#424242">'''Do you offer the option to lease or rent your instruments?'''</span>
<div class="mw-collapsible-content">
It is possible to lease the O2k over the course of three years, after which the instrument passes over into your ownership. Furthermore, you can rent an instrument for a short period (e.g. several weeks or months) if you need to improve your throughput for a specific study or experiment. Contact our office for further details: [mailto:[email protected] [email protected]]</div>
</div>
<div class="toccolours mw-collapsible mw-collapsed" style="background:#E6F8E0">
<span style="font-size:105%; color:#424242">'''Are you registered with System for Award Management (SAM)?'''</span>
<div class="mw-collapsible-content">
Yes.</div>
</div>


1. '''Q: Got confused with o2k-core and o2k-module? If I understood it right, o2k-core if purchased can be converted in future to measure other functions for example fluorescence, Ca+2, pH.'''
1. '''Q: Got confused with o2k-core and o2k-module? If I understood it right, o2k-core if purchased can be converted in future to measure other functions for example fluorescence, Ca+2, pH.'''

Revision as of 11:34, 25 April 2017

TEST:

TESTSEITE

OROBOROS FAQ



1. Q: Got confused with o2k-core and o2k-module? If I understood it right, o2k-core if purchased can be converted in future to measure other functions for example fluorescence, Ca+2, pH.


A: The O2k-Core is the actual instrument with the accessories, syringes and the ISS suction system. With the O2k-Core you can measure respiration, with the O2k-Modules you are able to measure different parameters as well. The Modules are ready to use with the O2k-Core without any special upgrading needed.

2. Q: How about installation of the machine in the lab here in US? Is anybody going to do it for us? Or, buyer has to do it.


A: Set-up and installation is performed by the customer with the help of our detailed and userfriendly O2k-Manual. Our customers from all over the world have been finding this very easy and our Manual is also freely available for download online. Please see: http://www.oroboros.at/index.php?o2k-manual. The manual, as well as the troubleshooting section on our wiki-based website (http://www.bioblast.at/index.php/Troubleshooting) and our technical specialists help to locate and solve any technical problems that might appear. Any parts required for repair onsite are shipped to the customer. For any grave technical faults which cannot be solved on site, the O2k is shipped to our workshop, where it is repaired.

3. Q: What is the advantage of titration-injection micropump? Is manual pipetting to add sample to the chamber difficult? How is it different from ISS-integrated suction system and microsyringe set in O2K-core?


A: TIP2k: Basically, the TIP2k makes life easier and work more comfortable, like automated determinations of oxygen background. If you are interested in e.g oxygen kinetics at low oxygen or very precise small step uncoupler titration, then the more advanced features of the TIP2k are necessary. If you want to use the O2k-Modules mentioned above were strongly recommend purchasing also the TIP2k. Most MultiSesnor measurements are also possible without it, but it gets dedious. For more information please have a look at the following link (http://www.oroboros.at/index.php?TIP2k). ISS: The ISS is for siphoning off excess medium from the O2k-Stopper and for emptying the O2k-Chambers. The ISS is included in the purchase of an O2k-Core and this suction pump is essential for the everyday usage of the instrument. The Microsyringe Set is another O2k-Core-accessory used for manual titrations.

4. Q: Are fluorescence sensors used for only green and blue emission? Is sensor not suitable for red emission? How about the excitation? Does the machine use a wide excitation spectrum?


A: You are correct, there was a confusion of excitation and emmision on our webpage. I have corrected this on http://wiki.oroboros.at/index.php/O2k-Fluorescence_LED2-Module. Thank you for bringing this to our attention!. Please note that the applications currently envisaged are listed on the top of the linked page (under description). We do not use a wide exciation but separate LED sources for the two mentioned excitation colors.

5. Q:If only Ca+2 is detected with the suitable module? Or, other ions such Na+, phosphate, K+ can also be detected.


A: I tried to answer your question here: http://wiki.oroboros.at/index.php/Talk:O2k-TPP%2B_and_Ca2%2B_ISE-Module

6. Q: Is special organ tissue or cell preparation needed to measure ions? Or, whole tissue and adherent/suspended cells are used.


A: Adherent cells (without bringing them in suspension are conceptional not well suited for our system, see http://wiki.oroboros.at/index.php/Adherent_cells. TPP+ has been measured now with three types of samples, isolated mitochondria, permabilized cultures cells and permeabilized tissues. See http://wiki.oroboros.at/index.php/Mitochondrial_membrane_potential for an overview. We have comparable little experience with Ca2+, please see http://wiki.oroboros.at/index.php/Calcium_electrode. I do not see any reason that would exclude a type of sample here.

7. Q: Where do you get NO sensor and the cost of it?


A: NO-Sensors: NO and H2O2 sensors use an amperometric measurement principle (Amp-Module), comparable to the polarographic oxygen sensor (POS), which yields an amperometric signal at a given polarization voltage. We supply custom-made stoppers that can accommodate such sensors. All amperometric sensors, like (H2S, H2O2) that have the same connection, can be used with the O2k. However, we have to mention that, while NO is measured by quite a few of our customers, the usability of any H2O2 electrode currently on the market is sometimes questioned. However, this depends on your sensitivity needs. We offer the O2k-MultiSensor Stoppers, for further information please check the following link: http://www.bioblast.at/index.php/O2k-NO_Amp-Module. For prices of NO electrodes (shaft with 2 mm diameter) please consult the manufacturer, e.g. WPI. WPI's 2 mm electrode is called "ISO-NOP" You will need the electrode housing , "replacement" sleeves #5436 (there are non with just the housing) and filling solution #7325. That is, unless they have changed the composition of their packages, so please inquire with them. For compatibility with our system the important point is the diameter of the sensor: 2mm.

8. Q: How are we going to avail parts and labor warranty in the US?


A: With our general terms and conditions we have the following warranty: 24 months* for parts and labour from day of delivery, except glassware and consumables. (*This warranty period applies in conjunction with participation in an Oxygraph-2k Course within 12 months of delivery. Otherwise the warranty period is reduced to 12 months.). To this, we can ship spare parts to you if you require any and if there is anything serious with the instrument it can be shipped to our Workshop.

9. Q: Please explain Delivery included but duty unpaid? I do not get it.


A: DDU (Delivery included - Duty Unpaid) means: We pay for the shipment (delivery) of your order and you pay for the local VAT and the customs duty for the import of the instrument.

10. Q: Different module mentioned includes o2k-chamber and oxygen sensor as spare. Do these go bad with usage? Do you recommend to have them as a backup?


A: O2k-chamber and Oxygen sensor: It is not required to have these items as backup however as they are quite essential to the usage of the instrument we recommend having them as spares. As the O2k-Chamber is out of glass, it may be good to have a backup, but not necessary. This is completely up to you. The oxygen sensor (POS) has a two year warranty and we recommend regular sensor maintenance to keep its lifetime to a maximum. POS maintenance: The sensors do not need to be shipped. For details please check our POS Service Manual which is freely available on our homepage (http://www.oroboros.at/index.php?o2k-pos-service.).

11. Q: With regard to workshop: If O2K-fluorescence is different from O2K-basic. What will be covered in the workshop in Sydney, Australia? Is registration fee included with the purchase for the workshop in Sydney?


A: The registration fee which is included with the purchase of the O2k-Core is valid for any O2k-Basic-Workshop. The Workshop in Sydney is an O2k-Basic-Workshop so you are free to go there if you prefer this destination. The registration for the Fluorescence-Workshop comes with the purchase of the Fluoresence-Module.