User talk:Tindle Lisa/My sandbox Open Support

                  

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User talk:Tindle Lisa/My sandbox Open Support

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Many inquiries we receive relate to an abnormal O₂ signal. In many of these cases, the issues can be diagnosed with a few simple checks. Therefore, please review the startup information and Quality Control steps/O2k-Open Support resources before contacting our O2k-Experts.

(You may also find the answers to more advanced topics using the navigation menu above or simply by using the Bioblast search function.)

Currently Asked Questions - »O2k-Open Support alert« 

O2k-Open Support resources

Startup information

• Startup checklist

• Installation and startup session reading material

- To register for your remote session with an O2k-Expert (included in with all O2k-Packages from May 2021 onward): Registration form

Quality control

• QC page

» Search for defective O2k components

Advanced topics

• Workshop reading material

• See navigation menu

Startup Information

• Startup checklist

• Installation and startup session reading material

- To register for your remote session with an O2k-Expert (included in with all O2k-Packages from May 2021 onward): Registration form

O2k-Quality Control

Many commonly raised issues of O2k-technical support are solved in the newest version of DatLab.

The O₂ sensor and O2k chamber tests are important to eliminate instrumental artefacts (instrumental quality control, QC), distinguished from assay QC addressing problems of solution protocols (preparation of chemicals), sample preparation protocols (such as mitochondrial preparations), and SUIT protocols.

1. Clean O2k-chambers and contamination-free incubation medium are required.
2. Analysis of DatLab files recorded in instrumental QC tests is essential.
3. Biological experiments are not suitable for trouble shooting.

1. O₂ sensor test

The OroboPOS (polarographic oxygen sensor) requires regular service at intervals - these may be more than 1 year. SOPs are available to determine if a sensor service is required at an earlier or later date:

» O₂ sensor test: QC1: Oxygen sensor test

2. O2k-chamber test

If all quality control criteria of the O₂ sensor test are met, the operator can be assured that the quality of the sensor signal is acceptable. Next, the quality of the O2k-chamber assembly has to be tested, described in detail:

» O2k-chamber test: QC2: Instrumental O₂ background test

Ask an O2k-Expert and the O2k-Open Support agreement

We give you the tools to be self-sufficient, but sometimes you need expert advice or help solving a technical problem. The Oroboros experts will be happy to help in using our O2k-Open Support system. » Contact Support

» O2k-Open Support agreement

» O2k repair

The O2k-Network: An additional resource

The Oroboros O2k-Network serves to connect and support: Contact for advice on applications of the O2k and high-resolution respirometry - the global network in mitochondrial physiology.

» The O2k-Network Labs: » O2k-Network

» O2k-Network discussion forum

» Oroboros O2k-Laboratory - open for innovation and cooperation.

» Oroboros Science Scholarships - are offered for focused research projects on mitochondrial physiology and high-resolution respirometry

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