From Bioblast
Description
Glucose, also known as D-glucose or dextrose, is a monosaccharide and an important carbohydrate in biology. Cells use it as the primary source of energy and a metabolic intermediate.
Abbreviation: Glc
Reference: MiPNet15.09. Yeast: HRR Reference Assay
Application in HRR
- Glc: Glucose (D-(+)-Glucose; C6H1206), Sigma-Aldrich: G8270, store at RT, CAS: 50-99-7, M = 180.16 g·mol-1
- 2021-03: The preparation instructions were updated to take the volume of the solute into account (see: Volume of the solute).
- Preparation of 2 M stock solution (1 mL, dissolved in H2O):
- weigh 360.32 mg of Glucose
- dissolve in 775 µL H2O in 1.5 mL Eppendorf
- »O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 20 µL using a 50 µL Hamilton syringe.
- Titration volume (0.5-mL O2k-chamber): 5 µL using a 25 µL Hamilton syringe.
- Final concentration: 20 mM
- Preparation of 2.2 M stock solution (200 µL, dissolved in H2O):
- weigh 79.2 mg of Glucose
- dissolve 155 µL H2O in 0.5 mL Eppendorf
- »O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 5 µL using a 25 µL Hamilton syringe.
- Final concentration: 5.5 mM (1 g/L)
- »O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 10 µL using a 25 µL Hamilton syringe.
- Final concentration: 11 mM (2 g/L)
MitoPedia topics:
Substrate and metabolite