User talk:Spitzer Gudrun

Antimycin A version 1

Ama: Antimycin A (from Streptomyces sp.) Sigma A 8674, 25 mg, store at -20 °C: FW = 540

Caution: Very toxic. Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.

Preparation of 5 mM stock solution (dissolved in 99.9% ethanol) for 2-mL O2k-chamber:

1. Weigh 5.4 mg of antimycin A into a small glass vial. Caution: Ama is hygroscopic.
2. Add 2 mL ethanol.
3. Divide into 0.2 mL portions in clear glass vials.
4. Store at -20 °C.

» O2k manual titrations MiPNet09.12 O2k-Titrations

• Titration volume (2-mL O2k-chamber): 1 µL using a 10 µL Hamilton syringe.
• Final concentration: 2.5 µM.

Preparation of 1,25 mM stock solution (dissolved in 99.9% ethanol) for 0.5-mL O2k-chamber:

1. Take 100 µL of 5 mM stock solution.
2. Add 300 µL ethanol.

» O2k manual titrations MiPNet09.12 O2k-Titrations

• Titration volume (0.5-mL O2k-chamber): 1 µL using a 10 µL Hamilton syringe.
• Final concentration: 2.5 µM.

Antimycin A version 2

Ama: Antimycin A (from Streptomyces sp.) Sigma A 8674, 25 mg, store at -20 °C: FW = 540

Caution: Very toxic. Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.

Preparation of 5 mM stock solution (dissolved in 99.9% ethanol):

1. Weigh 5.4 mg of antimycin A into a small glass vial. Caution: Ama is hygroscopic.
2. Add 2 mL ethanol.
3. Divide into 0.2 mL portions in clear glass vials.
4. Store at -20 °C.
5. For 1.25 mM concentration take 100 µL stock solution (5 mM) and dilute it with 300 µl ethanol.

» O2k manual titrations MiPNet09.12 O2k-Titrations

• Titration volume (2-mL O2k-chamber): 1 µL (5 mM) using a 10 µL Hamilton syringe.
• Titration volume (0.5-mL O2k-chamber): 1 µL (1.25 mM) using a 10 µL Hamilton syringe.
• Final concentration: 2.5 µM.