MiP2005: Session 7

Mitochondrial Physiology Network 10.9: 91 (2005) - download pdf

 

Influence of oxidative stress on osteoblasts cultured on either titanium or polystyrene.

Marie Kalbacova, U. Hempel, P. Dieter

Institute of Physiological Chemistry, Medical Faculty of Carl Gustav Carus, Dresden University of Technology. - marie.kalbacova@mailbox.tu-dresden.de

    Titanium is a successful biomaterial used for many applications that possesses good biocompatibility and mechanical strength. It is covered by a surface layer of titanium dioxide. Mechanical disintegration of this stable inert oxide layer leads to fast reformation of titanium dioxide (corrosion). For that, the oxidation of titanium (anodic reaction) as well as the reduction of oxygen (cathodic reaction) is necessary. During the cathodic reaction at least intermediately, reactive oxygen species (ROS) can occur, which are able to affect interactions between titanium implant and surrounding tissue (monocytes/macrophages, osteoblasts, osteoclasts, endothelial cells). The surrounding cells can be activated (firstly transiently and secondly permanently) and production of reactive oxygen species by cells themselves can be induced.

    In this study, we simulated oxidative stress by hydrogen peroxide and observed changes which occured in osteoblasts cultured on either titanium or polystyrene. We determined dose-dependent but material independent cytotoxicity, different reduced glutathione levels and activation of different enzymes of oxidative defence and intracellular signalling.

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