Boehmert 2020 Transfusion
|Böhmert S, Kübel S, Müller MM, Weber CF, Adam EH, Dröse S, Zacharowski K, Fischer D (2020) The effect of the interruption of agitation, temporary cooling, and pneumatic tube transportation on platelet quality during storage for transfusion. Transfusion [Epub ahead of print].|
Boehmert Stephanie, Kuebel Sarah, Mueller Markus Matthias, Weber Christian Friedrich, Adam Elisabeth Hannah, Droese Stefan, Zacharowski Kai, Fischer Dania (2020) Transfusion
Abstract: Conditions during blood product storage and transportation should maintain quality. The aim of this in vitro study was to investigate the effect of interruption of agitation, temporary cooling (TC), and pneumatic tube system transportation (PTST) on the aggregation ability (AA) and mitochondrial function (MF) of platelet concentrates (PC).
A PC was divided equally into four subunits and then allocated to four test groups. The control group (I) was stored as recommended (continuous agitation, 22 ± 2°C) for 4 days. The test groups were stored without agitation (II), stored as recommended, albeit 4°C for 60 minutes on day (d)2 (III) and PTST (IV). Aggregometry was measured using Multiplate (RocheAG; ADPtest, ASPItest, TRAPtest, COLtest) and MF using Oxygraph-2k (Oroboros Instruments). The basal and maximum mitochondrial respiratory rate (MMRR) were determined. AA and MF were measured daily in I and II and AA in III and IV on d2 after TC/PTST. Statistical analysis was performed using tests for matched observations.
Eleven PCs were used. TRAP-6 induced AA was significantly lower in II when compared to I on d4 (P = 0.015*). In III the ASPItest was significantly lower (P = 0.032*). IV showed no significant differences. The basal and MMRR were significantly reduced over 4 days in I and II (for both rates in both groups: P = <0.0001*). No significant differences occurred on d4 (P = 0.495).
Our results indicate that ex vivo AA and MF of PCs are unaffected, even in no-ideal storage and transport circumstances with respect to agitation, temperature, and force.
© 2020 The Authors. Transfusion published by Wiley Periodicals LLC. on behalf of AABB.
• Bioblast editor: Plangger M • O2k-Network Lab: DE Frankfurt Droese S
Labels: MiParea: Respiration, Instruments;methods
Organism: Human Tissue;cell: Platelet Preparation: Intact cells
Coupling state: LEAK, ROUTINE, ET Pathway: CIV HRR: Oxygraph-2k