Fisher 2020 J Physiol
|Fisher JJ, Vanderpeet CL, Bartho LA, McKeating DR, Cuffe JSM, Holland OJ, Perkins AV (2020) Mitochondrial dysfunction in placental trophoblast cells experiencing gestational diabetes mellitus. J Physiol 599:1291-305.
Abstract: Mitochondrial dysfunction has been associated with diabetic phenotypes, yet the involvement of placental mitochondria in gestational diabetes mellitus (GDM) remains inconclusive. This is in part complicated by the different mitochondrial subpopulations present in the two major trophoblast cell lineages of the placenta. To better elucidate the role of mitochondria in this pathology, this study examined key aspects of mitochondrial function in placentae from healthy pregnancies and those complicated by GDM in both whole tissue and isolated mitochondria. Mitochondrial content, citrate synthase activity, reactive oxygen species production and gene expression regulating metabolic, hormonal and antioxidant control was examined in placental tissue, before examining functional differences between mitochondrial isolates from cytotrophoblast (Cyto-Mito) and syncytiotrophoblast (Syncytio-Mito). Our study observed evidence of mitochondrial dysfunction across multiple pathways when assessing whole placental tissue from GDM pregnancies compared to healthy controls. Furthermore, by examining isolated mitochondria from the cytotrophoblast and syncytiotrophoblast cell lineages of the placenta we established that although both mitochondrial populations were dysfunctional, they were differentially impacted. These data highlight the need to consider changes in mitochondrial sub populations at the feto maternal interface when studying pregnancy pathologies. This article is protected by copyright. All rights reserved. • Keywords: Cytotrophoblast, Gestational diabetes mellitus, Mitochondrial dysfunction, Mitochondrial isolation, Placenta, Syncytiotrophoblast • Bioblast editor: Plangger M • O2k-Network Lab: AU Southport Peart J
Labels: MiParea: Respiration Pathology: Diabetes
Organism: Human Tissue;cell: Genital Preparation: Isolated mitochondria
Coupling state: OXPHOS, ET Pathway: N, NS, ROX HRR: Oxygraph-2k