Least squares method

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Bioblasts - Richard Altmann and MiPArt by Odra Noel
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Least squares method

Description

This method makes use of all of the data points of the spectrum in order to quantify a measured spectrum with a reference spectrum of known concentration using a least squares method to match the measured spectrum with the reference spectrum. The technique results in improved accuracy compared with the use of only a few characteristic wavelengths.



MitoPedia methods: Fluorometry, Spectrophotometry