Mitochondrial Preservation Medium, MiP03, developed for preservation of isolated mitochondria.
MitoPedia topics: Media for respirometry
- For preparation of MiP03 see MiPNet03.02 Chemicals-Media.
- 2 mM (+)-α-Tocopherol acid succinate (T3126, Sigma Aldrich, store at RT): dissolve 1.0616 mg in 1 ml Ethanol (100%) and store aliquots at 4 °C in dark glass vials (stable for several months)
- 1 mM Leupeptine (L9783, Sigma Aldrich, store at -20 °C): dissolve 0.463 mg in 1 ml H2O and store frozen aliquots at -20 °C (at least stable for 6 months)