MitoOx1

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MitoPedia

MitoOx1

Description

Mitochondrial respiration medium, MitoOx1, used by the Budapest groups for respirometry und Amplex Red trials.

Abbreviation: MitoOx1

Reference: Komary 2010 Biochim Biophys Acta






MitoPedia topics: Media for respirometry 

Application in HRR

MitoOx1: Mitochondrial Respiration Medium for measuring the H2O2 production

Oxygen solubility factor of MitoOx1 at 30 °C and 37 °C = 0.95


Chemicals for MitoOx1

Compound Final conc. M [g·mol-1] Company, product code and storage
KCl 125 mM 74.55 Sigma 31248, store at RT
HEPES free acid 20 mM 238.31 Sigma H-7523 store at RT
KH2PO4 2 mM 136.09 Sigma P-5655, store at RT
MgCl2 2.86 mM 203.30 Scharlau MA0036, store at RT
EGTA free acid 0.2 mM 380.35 Sigma E-4378, store at RT
BSA essentially fatty acid free 0.025% - Sigma A-6003, store at 2 - 8 °C

adjust pH to 7.0 (room temperature) with KOH