Mitochondrial preparations (mtprep) are isolated mitochondria (imt), tissue homogenate (thom), mechanically or chemically permeabilized tissue (permeabilized fibers, pfi) or permeabilized cells (pce). In mtprep the plasma membranes are either removed (imt) or mechanically (thom) and chemically permeabilized (pfi), while mitochondrial functional integrity and to a large extent mt-structure are maintained in incubation media optimized to support mitochondrial physiological performance. According to this definition, submitochondrial particles (smtp) are not a mtprep, since mitochondrial structure is altered although specific mitochondrial functions are preserved.
Communicated by Gnaiger E 2011-11-15 (last edit 2022-12-11).
- Isolated mitochondria, imt
- Tissue homogenate, thom
- Permeabilized tissue, pti
- Permeabilized muscle fibers, pfi
- Permeabilized cells, pce
- » Compare: Submitochondrial particles, smtp
- O2k-Protocols for mt-preparations: O2k-mitochondrial preparations
|BEC 2020.1 doi10.26124bec2020-0001.v1||Gnaiger E et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. https://doi.org/10.26124/bec:2020-0001.v1||2020|
Preparation: Isolated mitochondria, Permeabilized tissue, Permeabilized cells, Homogenate
MitoPedia topics: Sample preparation