Ouabain (synonym: G-strophantin octahydrate) is a poisonous cardiac glycoside. The classical mechanism of action of ouabain involves its binding to and inhibition of the plasma membrane Na+/K+-ATPase (sodium pump) especially at the higher concentrations. Low (nanomolar and subnanomolar) concentrations of ouabain stimulate the Na-K-ATPase.
Application in HRR
- Oua: Ouabain octahydrate (≥95% (HPLC), powder; C29H44O12*8H20); Sigma O 3125, store at R.T.; M = 728.77 g·mol-1.
- Caution: Light sensitive and toxic!
- Preparation of 10 mM stock solution (dissolved in H2O):
- Weigh 7.3 mg ouabain.
- Dissolve in 1 mL H2O.
- Divide into 0.2 mL portions or store in 2 mL glass vial and use subsamples during experiments to avoid contamination of the storage solution.
- Store in glass vials (recommendation) at -20 °C.
MitoPedia topics: Inhibitor