Parey 2018 Elife

From Bioblast
Publications in the MiPMap
Parey K, Brandt U, Xie H, Mills DJ, Siegmund K, Vonck J, KΓΌhlbrandt W, Zickermann V (2018) Cryo-EM structure of respiratory complex I at work.

Β» Elife 7:e39213. PMID: 30277212 Open Access

Parey Kristian, Brandt Ulrich, Xie Hao, Mills Deryck J, Siegmund Karin, Vonck Janet, Kuehlbrandt Werner, Zickermann Volker (2018) Elife

Abstract: Mitochondrial complex I has a key role in cellular energy metabolism, generating a major portion of the proton motive force that drives aerobic ATP synthesis. The hydrophilic arm of the L-shaped ~1 MDa membrane protein complex transfers electrons from NADH to ubiquinone, providing the energy to drive proton pumping at distant sites in the membrane arm. The critical steps of energy conversion are associated with the redox chemistry of ubiquinone. We report the cryo-EM structure of complete mitochondrial complex I from the aerobic yeast Yarrowia lipolytica both in the deactive form and after capturing the enzyme during steady-state activity. The site of ubiquinone binding observed during turnover supports a two-state stabilization change mechanism for complex I. β€’ Keywords: Yarrowia lipolytica, Active/deactive transition, Molecular biophysics, Redox-linked proton translocation, Respiratory complex I, Structural biology β€’ Bioblast editor: Plangger M

Labels: MiParea: mt-Structure;fission;fusion 

Enzyme: Complex I 

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