Salicylhydroxamic acid

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Bioblasts - Richard Altmann and MiPArt by Odra Noel
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Salicylhydroxamic acid


Salicylhydroxamic acid (SHAM; synonym: 2-Hydroxybenzohydroxamic acid N,2-Dihydroxybenzamide) is an inhibitor of the alternative oxidase (AOX). When AOX is blocked by SHAM, electrons are forced through the CIII-cytochrome c oxidase pathway, allowing observation of the operation of the CIII-CIV pathway without AOX activity.

Abbreviation: SHAM

Reference: Parrino_2000_J_Exp_Biol

Application in HRR

SHAM: Salicylhydroxamic acid (99%; HOC6H4CONHOH); Sigma S 607, 5 g, store at RT; M = 153.14 g·mol-1.
SHAM is reported to be easily soluble in EtOH. So EtOH could be considered as a solvent, too. However, in Oroboros-lab SHAM in EtOH has not been tested yet.
Caution: Irritant!
Preparation of 250 mM stock solution (dissolved in DMSO):
  1. Weigh 76.57 mg of SHAM.
  2. Dissolve in 1950 µL of DMSO.
  3. Divide into 0.2 mL portions
  4. Store at -20 °C.
» O2k Manual Titrations: MiPNet09.12 O2k-Titrations
  • Titration volume: 8 µL
  • Final conc. in 2 mL O2k chamber: 1 mM

MitoPedia topics: Inhibitor