Sodium phosphate buffer

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Sodium phosphate buffer

Description

Sodium phosphate buffer, Na-PB, for HRR with freeze-dried baker´s yeast.

Abbreviation: Na-PB



MitoPedia topics: Media for respirometry 

Application in HRR

  • Oxygen solubility factor of Na-PB = 0.95


Na-PB (50 mM, pH 7.1)

Dissolve 3.9 g NaH2PO4 x 2 H2O (Sigma 71505, M = 156.01 g·mol-1) in 500 mL a.dest and adjust pH with 10 M NaOH (Sigma 72068, M = 40.00 g·mol-1) to 7.1.