Oroboros Laboratory: Difference between revisions
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[[File:OROBOROS MitoFit-Lab.jpg|left|240px |MitoFit Laboratory]] | [[File:OROBOROS MitoFit-Lab.jpg|left|240px |MitoFit Laboratory]] | ||
[[Image:Logo MitoFit.jpg|right|180px|link=http://www.mitofit.org/index.php/MitoFit|MitoFit]] | [[Image:Logo MitoFit.jpg|right|180px|link=http://www.mitofit.org/index.php/MitoFit|MitoFit]] | ||
__TOC__ | __TOC__ | ||
The OROBOROS MitoFit Laboratory (MitoFit Lab, 'Mitochondriales Forschungslabor') is a scientific research laboratory in Innsbruck, Austria, that is probably the world-wide best equipped lab for high-resolution respirometry. The MitoFit Lab was opened during the course of the K-Regio project [[MitoCom_O2k-Fluorometer| MitoCom Tyrol]], June 2013. It comprises 140 sqm which are divided into the office and research areas. Β | Β | ||
:::: The OROBOROS MitoFit Laboratory (MitoFit Lab, 'Mitochondriales Forschungslabor') is a scientific research laboratory in Innsbruck, Austria, that is probably the world-wide best equipped lab for high-resolution respirometry. The MitoFit Lab was opened during the course of the K-Regio project [[MitoCom_O2k-Fluorometer| MitoCom Tyrol]], June 2013. It comprises 140 sqm which are divided into the office and research areas. Β | |||
=== O2k instruments === | === O2k instruments === | ||
[[File:OROBOROS MitoFit Lab IMG 7638.JPG|right|500px |Lisi & Yvonne, MitoFit Laboratory]] | [[File:OROBOROS MitoFit Lab IMG 7638.JPG|right|500px |Lisi & Yvonne, MitoFit Laboratory]] | ||
'''28 O2k-Chambers (14 O2k)''' | :::: '''28 O2k-Chambers (14 O2k)''' | ||
* Twelve O2k instruments (24 chambers) equipped with automatic Titration-Injection microPumps and the newest O2k-MultiSensor Modules or the NextGen-O2k configuration are available for research and biomedical applications. The newest instrumental designs are used for rigorous testing of our innovations. | ::::* Twelve O2k instruments (24 chambers) equipped with automatic Titration-Injection microPumps and the newest O2k-MultiSensor Modules or the NextGen-O2k configuration are available for research and biomedical applications. The newest instrumental designs are used for rigorous testing of our innovations. | ||
* Two additional O2ks (4 chambers) are devoted to instrumental development. | :::: * Two additional O2ks (4 chambers) are devoted to instrumental development. | ||
''More details:'' [[O2k-technical_support_and_open_innovation|OROBOROS Support]] | ::::Β» ''More details:'' [[O2k-technical_support_and_open_innovation|OROBOROS Support]] | ||
=== OXPHOS analysis === | === OXPHOS analysis === | ||
SUIT protocols are applied in tissue samples from animal models and human patients. Using cultured cells or cells extracted from blood samples, the effects of genotypic variations or of various drugs are studied on OXPHOS parameters, using | :::: SUIT protocols are applied in tissue samples from animal models and human patients. Using cultured cells or cells extracted from blood samples, the effects of genotypic variations or of various drugs are studied on OXPHOS parameters, using | ||
Β | |||
* various substrate-uncoupler-inhibitor titration (SUIT) protocols in permeabilized cells for deep OXPHOS phenotyping ([[Cell_ergometry|cell ergometry]]). | ::::* coupling control protocols in intact cells to evaluate effects on ROUTINE respiration, biochemical coupling efficiency and extracellular ROS production under physiological conditions; | ||
::::* various substrate-uncoupler-inhibitor titration (SUIT) protocols in permeabilized cells for deep OXPHOS phenotyping ([[Cell_ergometry|cell ergometry]]). | |||
=== The MitoFit Centre of Excellence === | === The MitoFit Centre of Excellence === | ||
OROBOROS INSTRUMENTS collaborates closely with research labs of the Medical University of Innsbruck, the Ludwig-Franzens University of Innsbruck, and with visiting scientists from numerous internation research institutions. | :::: OROBOROS INSTRUMENTS collaborates closely with research labs of the Medical University of Innsbruck, the Ludwig-Franzens University of Innsbruck, and with visiting scientists from numerous internation research institutions. | ||
According to the concept of open innovation, the Mitochondrial Research Laboratory welcomes visiting researchers from all over the world. | :::: According to the concept of open innovation, the Mitochondrial Research Laboratory welcomes visiting researchers from all over the world. | ||
[[File:ORO Lab 3.jpg|250px]] [[File:ORO Lab 4.jpg|250px]] [[File:ORO Lab 2.jpg|250px]] | [[File:ORO Lab 3.jpg|250px]] [[File:ORO Lab 4.jpg|250px]] [[File:ORO Lab 2.jpg|250px]] | ||
::::Β» [[OROBOROS_Company#Current_projects |'''Current projects and collaborators''']] | |||
Β | ::::Β» [[OROBOROS_MitoFit_lab:_visiting_scientists |'''Visiting scientists''']] | ||
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:::: [[OROBOROS_MitoFit_lab:_visiting_scientists |''' |
Revision as of 18:37, 3 November 2016
Oroboros Laboratory
- The OROBOROS MitoFit Laboratory (MitoFit Lab, 'Mitochondriales Forschungslabor') is a scientific research laboratory in Innsbruck, Austria, that is probably the world-wide best equipped lab for high-resolution respirometry. The MitoFit Lab was opened during the course of the K-Regio project MitoCom Tyrol, June 2013. It comprises 140 sqm which are divided into the office and research areas.
O2k instruments
- 28 O2k-Chambers (14 O2k)
- Twelve O2k instruments (24 chambers) equipped with automatic Titration-Injection microPumps and the newest O2k-MultiSensor Modules or the NextGen-O2k configuration are available for research and biomedical applications. The newest instrumental designs are used for rigorous testing of our innovations.
- * Two additional O2ks (4 chambers) are devoted to instrumental development.
- Β» More details: OROBOROS Support
OXPHOS analysis
- SUIT protocols are applied in tissue samples from animal models and human patients. Using cultured cells or cells extracted from blood samples, the effects of genotypic variations or of various drugs are studied on OXPHOS parameters, using
- coupling control protocols in intact cells to evaluate effects on ROUTINE respiration, biochemical coupling efficiency and extracellular ROS production under physiological conditions;
- various substrate-uncoupler-inhibitor titration (SUIT) protocols in permeabilized cells for deep OXPHOS phenotyping (cell ergometry).
The MitoFit Centre of Excellence
- OROBOROS INSTRUMENTS collaborates closely with research labs of the Medical University of Innsbruck, the Ludwig-Franzens University of Innsbruck, and with visiting scientists from numerous internation research institutions.
- According to the concept of open innovation, the Mitochondrial Research Laboratory welcomes visiting researchers from all over the world.