Bioblast alert 2015: Difference between revisions

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=== Bioblast alert 2011(04): 2011-12-24 ===
=== Bioblast alert - previous issues ===


* '''Congratulations''' to [[Pesta D|Dr. Dominik Pesta]], for receiving his degree of a PhD at the Medical University of Innsbruck! (Jan 2012)
* [[Bioblast alert 2011]]
* [[Bioblast editorial board]] - [[Talk:Bioblast editorial board|Why a wiki editorial board?]]
* '''[[SUIT protocol]]s for HRR: from intact cells to permeabilized muscle fibres''':
::[[Pesta 2012 Methods Mol Biol|Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58.]]
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=== Bioblast alert 2011(03): 2011-11-24 ===
* '''Fluorometry combined with high-resolution respirometry''': Light guide inserted into the chamber through a black PEEK stopper<ref>[[Anderson 2011 Am J Physiol Heart Circ Physiol|Anderson EJ, Rodriguez E, Anderson CA, Thayne K, Chitwood WR, Kypson AP (2011) Increased propensity for cell death in diabetic human heart is mediated by mitochondrial-dependent pathways. Am J Physiol Heart Circ Physiol 300: H118-124.]]</ref>, or optical probe operated through the O2k-glass window<ref>[[Hickey 2012 J Comp Physiol B|Hickey AJ, Renshaw GM, Speers-Roesch B, Richards JG, Wang Y, Farrell AP, Brauner CJ (2012) A radical approach to beating hypoxia: depressed free radical release from heart fibres of the hypoxia-tolerant epaulette shark (''Hemiscyllum ocellatum''). J Comp Physiol B [Epub ahead of print] ]]</ref>.
<references/>
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*'''[[Talk:Fluorometry|Fluorometry versus fluorimetry]]''' - term of the week. >>>New '''[[Glossary: Fluorometry]]''' - high-resolution terminology.
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=== Bioblast alert 2011(02): 2011-11-17 ===
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* '''[http://www.oroboros.at/index.php?ioc64 O2k-Workshop IOC64 - Centre for Cellular and Molecular Biology, Hyderabad, India]: 2011-12-08'''.
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* '''Heart failure and mitochondrial dysfunction'''. Increased [[OXPHOS capacity]] after cold preservation of the human heart – a paradox resolved by a dyscoupling mechanism.
::[[Lemieux 2011 Int J Biochem Cell Biol|Lemieux H, Semsroth S, Antretter H, Hoefer D, Gnaiger E (2011) Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart. Int J Biochem Cell Biol 43: 1729–1738.]] --> [[Talk:Lemieux 2011 Int J Biochem Cell Biol|Discussion: Complementary results on the PGC-1a pathway]].
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* '''[[Tissue storage]]''' on ice is possible for limited periods of time (several hours) with preservation of mitochondrial respiratory function, as evaluated with [[SUIT protocol]]s. ([[Scientific service]])
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* '''[[Uncoupled]], [[non-coupled]], [[dyscoupled]]''' - term of the week: a hallmark of high-resolution respirometry is a matching high-resolution terminology.
::[[Talk:Uncoupled respiration|Evaluate an example of low-resolution terminology]]: '''''[[Dyscoupled respiration|Uncoupling]]''' was studied in stressed cells by measurement of coupled respiration, evaluation of '''[[LEAK|uncoupled]]''' respiration after inhibition with oligomycin, and FCCP titration to quantify '''[[non-coupled|uncoupled]]''' respiration''.
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=== Bioblast alert 2011(01): 2011-11-11 ===
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* A key question on the separation of '''qualitative versus quantitative change of mitochondrial function''' is addressed in a new O2k-Publication. Does training induce mild uncoupling? Are sedentary humans healthy controls?
::[[Pesta_2011_Am J Physiol Regul Integr Comp Physiol|Pesta D, Hoppel F, Macek C, Messner H, Faulhaber M, Kobel C, Parson W, Burtscher M, Schocke M, Gnaiger E (2011) Similar qualitative and quantitative changes of mitochondrial respiration following strength and endurance training in normoxia and hypoxia in sedentary humans. Am J Physiol Regul Integr Comp Physiol 301: R1078–R1087.]]
* [[ETS capacity]] plays a key role in such analyses, which cannot be achieved quantitatively without careful '''[[Optimum uncoupler concentration|uncoupler titrations]]'''.
* '''[[Basal respiration]]''' - term of the month: a hallmark of high-resolution respirometry is a matching high-resolution terminology.


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Revision as of 07:08, 31 October 2012

Bioblast alert.jpg

Bioblast alert is prepared by the Bioblast editorial board to draw attention to specific topics on the Bioblast wiki. 

Bioblast alert: for information purposes, not intended to be an advertisement.  
Contributions are welcome.


Bioblast alert 2012(07): 2012-10-24

  • MitoPedia topic: Publications of historical interest are valued as milestones in science, as sources of understanding currently discussed concepts, and as a reminder that 'nothing' is new (something new is 'nothing'?) >> Mitochondria and bioblasts: Made history
  • MitoPedia terminology: LEAK vs. State 2: The resting state of substrate supported respiration without ADP (LEAK state) in contrast to the substrate limited State 2 of residual oxygen consumption at high ADP concentration: >> Chance and Williams 1955.
MitoPedia Glossary: Respiratory states

Bioblast alert 2012(06): 2012-07-20

>> More information on the MitoPedia list Substrates and metabolites
  • MitoPedia terminology: State 3 - is [ADP] saturating? Not generally in State 3, but certainly in the OXPHOS state (P).
>> Update on the MitoPedia glossary: Respiratory states

Bioblast alert 2012(05): 2012-06-05

Bioblast alert 2012(04): 2012-04-29

Votion DM, Gnaiger E, Lemieux H, Mouithys-Mickalad A, Serteyn D (2012) Physical fitness and mitochondrial respiratory capacity in horse skeletal muscle. PLoS One 7(4): e34890.

Bioblast alert 2012(03): 2012-03-23 / 2012-04-03

MitoPedia topic: Oxygen dependence of ROS production - are permeabilized fibres a valid model?

--> Discussion

Drosophila vs. sandworm: High-resolution respirometry in permeabilized muscle fibres

  1. Pichaud N, Rioux P, Blier PU (2012) In situ quantification of mitochondrial respiration in permeabilized fibers of a marine invertebrate with low aerobic capacity. Comp Biochem Physiol A Mol Integr Physiol 161: 429-435.
  2. Pichaud N, Ballard JWO, Tanguay RM, Blier PU (2011) Thermal sensitivity of mitochondrial functions in permeabilized muscle fibers from two populations of Drosophila simulans with divergent mitotypes. Am J Physiol Regul Integr Comp Physiol 301: R48-R49.

New from the Ballard lab:


Bioblast alert 2012(02): 2012-03-06

  • 50 Years ago: Nick Holonyak, considered as the "father of the light-emitting diode", developed the first practical visible-spectrum LED in 1962. Which colour? What is a LED? >>> see Glossary: Fluorescence

Mitochondrial dys/function in obesity vs. anorexia:

  1. Lindfors C, Nilsson IA, Garcia-Roves PM, Zuberi AR, Karimi M, Donahue LR, Roopenian DC, Mulder J, UhlΓ©n M, EkstrΓΆm TJ, Davisson MT, HΓΆkfelt TG, Schalling M, Johansen JE (2011) Hypothalamic mitochondrial dysfunction associated with anorexia in the anx/anx mouse. Proc Natl Acad Sci U S A 108: 18108-18113.
  2. Holmstroem MH, Iglesias-Gutierrez E, Zierath JR, Garcia-Roves PM (2012) Tissue-specific control of mitochondrial respiration in obesity-related insulin resistance and diabetes. Am J Physiol Endocrinol Metab 302: E731-E739.

Bioblast alert 2012(01): 2012-02-27

Two exciting publications on mitochondrial p50, apparent excess capacity of cytochrome c oxidase, basal metabolic rate, P/O ratios and ergometric efficiency:

  1. Larsen FJ, Schiffer TA, Sahlin K, Ekblom B, Weitzberg E, Lundberg JO (2011) Mitochondrial oxygen affinity predicts basal metabolic rate in humans. FASEB J 25: 2843-2852.
  2. Larsen FJ, Schiffer TA, Borniquel S, Sahlin K, Ekblom B, Lundberg JO, Weitzberg E (2011) Dietary inorganic nitrate improves mitochondrial efficiency in humans. Cell Metab 13: 149-159.


Bioblast alert - previous issues

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