Template:Current week ecosystem agenda: Difference between revisions

Latest revision as of 09:37, 21 May 2024

Oroboros declares solidarity against the Russian aggression-war in Ukraine and against all aggression-wars worldwide. - »More details

-» A future for Ukraine is the future of its science

2024 Week 21

May-20 Mo

How do I determine the optimal oligomycin concentration for my assay?

Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity.
- »Oligomycin titration test«
- communicated by »Baglivo Eleonora« and »Schmitt Sabine«

May-21 Tu

May-22 We - International Day for Biological Diversity

"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."

Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. »Bioblast link«

from the O2k-Network »NO Tromsoe Wood SH«

May-23 Th

May-24 Fr

Next Week

2024 Week 22

May-27 Mo

Same experimental conditions, but different O₂ fluxes in the left and right chambers?

Check for instrumental and operational factors that could be causing O₂ flux differences between the O2k-chambers.
- »Different O2 fluxes in left and right chamber«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«

May-28 Tu

May-29 We

May-30 Th

"Harmonization of isolation protocols yielding intact and enzymatically active mitochondria are critical to assess mitochondrial respiratory function"

- »MiPNet20.06 Isolation mouse heart mitochondria«
- communicated by »Cardoso Luiza« and »Leo Elettra«

May-31 Fr

@@ Line 1: / Line 1: @@
+{{Template:Science for Ukraine}}
-Week 20
+Week 21
-* '''May-11 Mo'''
+* '''May-20 Mo'''
+:{|
+| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
+|''' How do I determine the optimal oligomycin concentration for my assay?'''<br>
+Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity. <br>
+- »'''[https://wiki.oroboros.at/index.php/Oligomycin#Omy_titration_test Oligomycin titration test]'''« <br>
+- communicated by »[[Baglivo Eleonora]]« and »[[Schmitt Sabine]]«
+|}
+----
+* '''May-21 Tu'''
+----
+* '''May-22 We''' - International Day for Biological Diversity
 :{|
-| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]
+| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-|'''Do you know how to avoid underestimating/overestimating your respiratory fluxes? Measuring steady states with high-resolution respirometry is the key to obtaining correct values.'''<br>
+|'''"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."''' <br>
-Learn more about steady states measured with high-resolution respirometry: performance and mark setting for data analysis <br>
+* Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. [[Ciccone 2024 J Exp Biol|»Bioblast link«]]
-- »[[Steady state]]«
+:from the O2k-Network »[[NO Tromsoe Wood SH]]«
 |}
 ----
-* '''May-12 Tu'''
+* '''May-23 Th'''
-:{|
+----
-| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
+* '''May-24 Fr'''
-| '''Mitochondria - a driving force in adipocyte differentiation''' <br>
-:from the O2k-Network »[[CZ Pilsen Kuncova J]]« <br>
-* Kladnická I, Čedíková M, Kripnerová M, Dvořáková J, Kohoutová M, Tůma Z, Müllerová D, Kuncová J (2019) Mitochondrial respiration of adipocytes differentiating from human mesenchymal stem cells derived from adipose tissue. Physiol Res 68:287-96. [[Kladnicka 2019 Physiol Res|»Bioblast link«]]
-|}
 ----
-* '''May-13 We'''
-----
+'''<big>Next Week</big>'''
-* '''May-14 Th'''
+Week 22
+* '''May-27 Mo'''
 :{|
-| width="110" align="center" | [[File:MitoPedia.jpg|left|60px|MitoPedia]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Info: take care]]
-|How can I measure '''ATP production''' with the O2k?  <br>
+|'''Same experimental conditions, but different O<sub>2</sub> fluxes in the left and right chambers?'''<br>
-Learn more about how to measure ATP production in [[mitochondrial preparations]] with Magnesium Green <br>
+Check for instrumental and operational factors that could be causing O<sub>2</sub> flux differences between the O2k-chambers.
-- »'''[[Magnesium Green]]'''«
+<br>
+- »'''[[Different O2 fluxes in left and right chamber]]'''«<br>
+- communicated by »[[Timon-Gomez Alba]]« and »[[Baglivo Eleonora]]«
 |}
 ----
-* '''May-15 Fr'''
+* '''May-28 Tu'''
+----
+* '''May-29 We'''
+----
+* '''May-30 Th'''
 :{|
-| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|''' Restoration of mitochondrial oxygen consumption and decrease of mitochondrial ROS by AOX in the post-ischaemic heart are not sufficient to confer acute or chronic cardioprotection in mouse ''' <br>
+|''' "Harmonization of isolation protocols yielding intact and enzymatically active mitochondria are critical to assess mitochondrial respiratory function"'''<br>
-: from the O2k-Network » [[ FI Helsinki Jacobs HT]] « , » [[ DE Magdeburg Gellerich FN]] « , » [[FI Tampere Dufour E]] « , » [[DE Magdeburg Debska-Vielhaber G]] « and » [[DE Frankfurt Wittig I]]«<br>
+- »'''[[MiPNet20.06_IsolationMouseHeart-mt|MiPNet20.06 Isolation mouse heart mitochondria]]'''«<br>
-* Szibor Marten, Schreckenberg Rolf, Gizatullina Zemfira, Dufour Eric, Wiesnet Marion, Dhandapani Praveen Kumar, Debska-Vielhaber Grazyna, Heidler Juliana, Wittig Ilka, Nyman Tuula A, Gaertner Ulrich, Hall Andrew R, Pell Victoria, Viscomi Carlo, Krieg Thomas, Murphy Michael P, Braun Thomas, Gellerich Frank Norbert, Schlueter Klaus-Dieter, Jacobs Howard T(2020) Respiratory chain signalling is essential for adaptive remodelling following cardiac ischaemia. J Cell Mol Med [Epub ahead of print].   - [[Szibor 2020 J Cell Mol Med|»Bioblast link«]]
+- communicated by »[[Cardoso Luiza]]« and »[[Leo Elettra]]«
 |}
+----
+* '''May-31 Fr'''
+----