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Latest revision as of 09:37, 21 May 2024

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-» A future for Ukraine is the future of its science

2024 Week 21

May-20 Mo

How do I determine the optimal oligomycin concentration for my assay?

Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity.
- »Oligomycin titration test«
- communicated by »Baglivo Eleonora« and »Schmitt Sabine«

May-21 Tu

May-22 We - International Day for Biological Diversity

"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."

Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. »Bioblast link«

from the O2k-Network »NO Tromsoe Wood SH«

May-23 Th

May-24 Fr

Next Week

2024 Week 22

May-27 Mo

Same experimental conditions, but different O₂ fluxes in the left and right chambers?

Check for instrumental and operational factors that could be causing O₂ flux differences between the O2k-chambers.
- »Different O2 fluxes in left and right chamber«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«

May-28 Tu

May-29 We

May-30 Th

"Harmonization of isolation protocols yielding intact and enzymatically active mitochondria are critical to assess mitochondrial respiratory function"

- »MiPNet20.06 Isolation mouse heart mitochondria«
- communicated by »Cardoso Luiza« and »Leo Elettra«

May-31 Fr

@@ Line 1: / Line 1: @@
 {{Template:Science for Ukraine}}
-Week 41
-*'''Oct- 09 Mo'''
+Week 21
-----
-*'''Oct- 10 Tu'''
+* '''May-20 Mo'''
 :{|
 | width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
-|'''What is the best fluorophore and concentration thereof for measuring mt-membrane potential?'''<br>
+|''' How do I determine the optimal oligomycin concentration for my assay?'''<br>
-It depends on sample characteristics -  test the concentration of different fluorophores (safranin, TMRM and rhodamine 123) with your specific model to evaluate for inhibitory or uncoupling effects on respiration. <br>
+Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity. <br>
-- »'''[https://wiki.oroboros.at/index.php/Mitochondrial_membrane_potential#Different_methods_for_measurements_of_mt-membrane_potential mt-membrane potential measurement]'''« <br>
+- »'''[https://wiki.oroboros.at/index.php/Oligomycin#Omy_titration_test Oligomycin titration test]'''« <br>
-- communicated by »[[Grings Mateus]]« and »[[Cardoso Luiza]]«
+- communicated by »[[Baglivo Eleonora]]« and »[[Schmitt Sabine]]«
 |}
 ----
-*'''Oct- 11 We'''
+* '''May-21 Tu'''
-:{|
-| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
-| The abbreviation 'Dith' has been used previously for '''Dithionite''' and is stepwise replaced by the shorter 'Dit'. '''<br>
-- »'''[https://wiki.oroboros.at/index.php/Dithionite Dithionite, Dit]'''«
-|}
 ----
-*'''Oct- 12 Th''' - World Arthritis Day
+* '''May-22 We''' - International Day for Biological Diversity
 :{|
 | width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-|'''“Mitochondrial dysfunction did not seem to be a major contributing factor for insulin resistance in RA [rheumatoid arthritis]”''' <br>
+|'''"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."''' <br>
-* Moellering DR, Smith-Johnston K, Kelley C, Sammy MJ, Benedict J, Brock G, Johnson J, Baskin KK, Jarjour WN, Belury MA, Reiser PJ, Nagareddy PR, Hanaoka BY (2023) Association between skeletal muscle mitochondrial dysfunction and insulin resistance in patients with rheumatoid arthritis: a case-control study. [[Moellering 2023 Arthritis Res Ther|»Bioblast link«]]
+* Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. [[Ciccone 2024 J Exp Biol|»Bioblast link«]]
-:from the O2k-Network »[[US AL Birmingham Moellering_DR]]«
+:from the O2k-Network »[[NO Tromsoe Wood SH]]«
 |}
 ----
-*'''Oct- 13 Fr'''
+* '''May-23 Th'''
+----
+* '''May-24 Fr'''
 ----
-'''<big>Next Week</big>
+'''<big>Next Week</big>'''
-'''
+Week 22
-Week 42
-*'''Oct- 16 Mo'''
+* '''May-27 Mo'''
-:::: '''»[[2nd Workshop on Mitochondrial Functional Diagnostics Innsbruck AT]]«'''
-----
-*'''Oct- 17 Tu'''
 :{|
-| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Info: take care]]
-|'''Noticing higher values of J°1 when using the O2k sV-chamber (0.5 mL) compared with the 2mL-chamber? '''<br>
+|'''Same experimental conditions, but different O<sub>2</sub> fluxes in the left and right chambers?'''<br>
-The instrumental oxygen background is volume-dependent in the closed chamber; therefore, the values of J°1 are about four times higher in the sV chamber.
+Check for instrumental and operational factors that could be causing O<sub>2</sub> flux differences between the O2k-chambers.
 <br>
-- »'''[[O2k-sV-Module|O2k-sV-Module]]'''«, »'''[[MiPNet06.03 POS-calibration-SOP|POS calibration-SOP]]'''«<br>
+- »'''[[Different O2 fluxes in left and right chamber]]'''«<br>
-- communicated by »[[Timon-Gomez Alba]]« and »[[Leo Elettra]]«
+- communicated by »[[Timon-Gomez Alba]]« and »[[Baglivo Eleonora]]«
 |}
 ----
-*'''Oct- 18 We'''
+* '''May-28 Tu'''
+----
+* '''May-29 We'''
 ----
-*'''Oct- 19 Th'''
+* '''May-30 Th'''
 :{|
-| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|'''"MDH2 is a metabolic switch rewiring [electron transfer system] and TCA fuelling"''' <br>
+|''' "Harmonization of isolation protocols yielding intact and enzymatically active mitochondria are critical to assess mitochondrial respiratory function"'''<br>
-* Molinié T, Cougouilles E, David C, Cahoreau E, Portais JC, Mourier A (2022) MDH2 produced OAA is a metabolic switch rewiring the fuelling of respiratory chain and TCA cycle. [[Molinie 2022 Biochim Biophys Acta Bioenerg|»Bioblast link«]]
+- »'''[[MiPNet20.06_IsolationMouseHeart-mt|MiPNet20.06 Isolation mouse heart mitochondria]]'''«<br>
-:from the O2k-Network »[[FR Bordeaux Mourier A]]«
+- communicated by »[[Cardoso Luiza]]« and »[[Leo Elettra]]«
 |}
 ----
-*'''Oct- 20 Fr'''
+* '''May-31 Fr'''
 ----