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Latest revision as of 09:37, 21 May 2024

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-» A future for Ukraine is the future of its science

2024 Week 21

May-20 Mo

How do I determine the optimal oligomycin concentration for my assay?

Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity.
- »Oligomycin titration test«
- communicated by »Baglivo Eleonora« and »Schmitt Sabine«

May-21 Tu

May-22 We - International Day for Biological Diversity

"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."

Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. »Bioblast link«

from the O2k-Network »NO Tromsoe Wood SH«

May-23 Th

May-24 Fr

Next Week

2024 Week 22

May-27 Mo

Same experimental conditions, but different O₂ fluxes in the left and right chambers?

Check for instrumental and operational factors that could be causing O₂ flux differences between the O2k-chambers.
- »Different O2 fluxes in left and right chamber«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«

May-28 Tu

May-29 We

May-30 Th

"Harmonization of isolation protocols yielding intact and enzymatically active mitochondria are critical to assess mitochondrial respiratory function"

- »MiPNet20.06 Isolation mouse heart mitochondria«
- communicated by »Cardoso Luiza« and »Leo Elettra«

May-31 Fr

@@ Line 1: / Line 1: @@
 {{Template:Science for Ukraine}}
-Week 44
+Week 21
-*'''Oct- 30 Mo'''
+* '''May-20 Mo'''
-----
-*'''Oct- 31 Tu'''
 :{|
 | width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
-|''' How to interpret cytochrome ''c'' efficiency '''<br>
+|''' How do I determine the optimal oligomycin concentration for my assay?'''<br>
-Stimulation of OXPHOS or ET capacity upon addition of cytochrome ''c''  provides evidence of cytochrome ''c'' loss, indicating damage of the mitochondrial outer membrane, which could be caused by sample preparation or pathological conditions. <br>
+Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity. <br>
-- »'''[https://wiki.oroboros.at/index.php/Cytochrome_c_control_efficiency Cytochrome ''c'' control efficiency]'''« <br>
+- »'''[https://wiki.oroboros.at/index.php/Oligomycin#Omy_titration_test Oligomycin titration test]'''« <br>
-- communicated by »[[Schmitt Sabine]]« and »[[Leo Elettra]]«
+- communicated by »[[Baglivo Eleonora]]« and »[[Schmitt Sabine]]«
 |}
 ----
-*'''Nov- 01 We'''
+* '''May-21 Tu'''
 ----
-*'''Nov- 02 Th'''
+* '''May-22 We''' - International Day for Biological Diversity
-----
+:{|
-*'''Nov- 03 Fr'''
-:{|
 | width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-|'''"Peripheral impairments at the skeletal muscle level, namely reduced mitochondrial function and the presence of markers of impaired mitochondrial biogenesis emerged as major determinants of exercise intolerance and fatigue [in patients with post-acute sequelae of SARS-CoV-2 infection]"''' <br>
+|'''"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."''' <br>
-* Colosio M, Brocca L, Gatti M, Neri M, Crea E, Cadile F, Canepari M, Pellegrino MA, Polla B, Porcelli S, Bottinelli R (2023) Structural and functional impairments of skeletal muscle in patients with post-acute sequelae of SARS-CoV-2 infection. [[Colosio 2023 J Appl Physiol (1985)|»Bioblast link«]]
+* Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. [[Ciccone 2024 J Exp Biol|»Bioblast link«]]
+:from the O2k-Network »[[NO Tromsoe Wood SH]]«
 |}
+----
+* '''May-23 Th'''
+----
+* '''May-24 Fr'''
 ----
 '''<big>Next Week</big>'''
+Week 22
-Week 45
+* '''May-27 Mo'''
-*'''Nov- 06 Mo'''
-----
-*'''Nov- 07 Tu'''
 :{|
 | width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Info: take care]]
-|'''How often should I re-assemble the O2k-chamber?'''<br>
+|'''Same experimental conditions, but different O<sub>2</sub> fluxes in the left and right chambers?'''<br>
-We recommend disassembling, cleaning and re-assembling the O2k-chamber at least every 1-2 years. It is also necessary to disassemble it when there is persistent biological or protein contamination, medium leakage from the chamber, an oxygen leak of the chamber, or an unstable or unexpectedly high or low oxygen signal.
+Check for instrumental and operational factors that could be causing O<sub>2</sub> flux differences between the O2k-chambers.
 <br>
-- »'''[https://wiki.oroboros.at/index.php/O2k-chamber#O2k-chamber_assembly_-_possible_problems O2k-chamber assembly - possible problems]'''« and »'''[https://www.youtube.com/watch?v=1MSsI1ePMu8&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3 O2k-Videosupport: Insert O2k-chamber]'''«<br>
+- »'''[[Different O2 fluxes in left and right chamber]]'''«<br>
-- communicated by »[[Timon-Gomez Alba]]« and »[[Schmitt Sabine]]«
+- communicated by »[[Timon-Gomez Alba]]« and »[[Baglivo Eleonora]]«
 |}
 ----
-*'''Nov- 08 We'''
+* '''May-28 Tu'''
 ----
-*'''Nov- 09 Th'''
+* '''May-29 We'''
 ----
-*'''Nov- 10 Fr'''
+* '''May-30 Th'''
 :{|
-| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|''' When should I analyze my data as Flux control ratios (''FCR''s)?'''<br>
+|''' "Harmonization of isolation protocols yielding intact and enzymatically active mitochondria are critical to assess mitochondrial respiratory function"'''<br>
-''FCR'' enables the comparison of samples normalized with various mitochondrial content parameters or when sample concentration is unknown. This is achieved by internally normalizing O<sub><small>2</small></sub> fluxes in different respiratory states to the maximum flux in a common reference state. <br>
+- »'''[[MiPNet20.06_IsolationMouseHeart-mt|MiPNet20.06 Isolation mouse heart mitochondria]]'''«<br>
-- »'''[[Flux control ratio]]'''« <br>
+- communicated by »[[Cardoso Luiza]]« and »[[Leo Elettra]]«
-- communicated by »[[Baglivo Eleonora]]« and »[[Timon-Gomez Alba]]«
 |}
+----
+* '''May-31 Fr'''
 ----