Template:Current week ecosystem agenda: Difference between revisions

Latest revision as of 09:37, 21 May 2024

Oroboros declares solidarity against the Russian aggression-war in Ukraine and against all aggression-wars worldwide. - »More details

-» A future for Ukraine is the future of its science

2024 Week 21

May-20 Mo

How do I determine the optimal oligomycin concentration for my assay?

Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity.
- »Oligomycin titration test«
- communicated by »Baglivo Eleonora« and »Schmitt Sabine«

May-21 Tu

May-22 We - International Day for Biological Diversity

"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."

Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. »Bioblast link«

from the O2k-Network »NO Tromsoe Wood SH«

May-23 Th

May-24 Fr

Next Week

2024 Week 22

May-27 Mo

Same experimental conditions, but different O₂ fluxes in the left and right chambers?

Check for instrumental and operational factors that could be causing O₂ flux differences between the O2k-chambers.
- »Different O2 fluxes in left and right chamber«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«

May-28 Tu

May-29 We

May-30 Th

"Harmonization of isolation protocols yielding intact and enzymatically active mitochondria are critical to assess mitochondrial respiratory function"

- »MiPNet20.06 Isolation mouse heart mitochondria«
- communicated by »Cardoso Luiza« and »Leo Elettra«

May-31 Fr

@@ Line 1: / Line 1: @@
 {{Template:Science for Ukraine}}
-Week 49
+Week 21
-*'''Dec- 04 Mo'''
+* '''May-20 Mo'''
-----
-*'''Dec- 05 Tu'''
 :{|
-| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
+| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
-|''' Is DatLab 8 compatible with your operative system?'''<br>
+|''' How do I determine the optimal oligomycin concentration for my assay?'''<br>
-The DatLab 8 software is designed for 64-bit versions of Windows operating systems and does not run on MAC devices.
+Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity. <br>
-<br>
+- »'''[https://wiki.oroboros.at/index.php/Oligomycin#Omy_titration_test Oligomycin titration test]'''« <br>
-- »'''[https://www.oroboros.at/index.php/product/datlab/ DatLab 8]'''«<br>
+- communicated by »[[Baglivo Eleonora]]« and »[[Schmitt Sabine]]«
-- communicated by »[[Leo Elettra]]« and »[[Baglivo Eleonora]]«
+|}
-|}
 ----
-*'''Dec- 06 We'''
+* '''May-21 Tu'''
-:{|
-| width="100" align="center" |[[File:MitoFit Preprints.png|100px|link=https://wiki.oroboros.at/images/0/00/Cecatto_2023_MitoFit.pdf |MitoFit pdf]]
-|'''"The capacity of FA oxidative phosphorylation (F-OXPHOS) with palmitoylcarnitine was up to 4 times higher than respiration with octanoylcarnitine. The optimal concentration of palmitoylcarnitine was 10 μM which corresponds to the total concentration of LC acylcarnitines in the brain."'''<br>
-* Cecatto C, Cardoso LHD, Ozola M, Korzh S, Zvejniece L, Gukalova B, Doerrier C, Dambrova M, Gnaiger E, Makrecka-Kuka M, Liepinsh E (2023) Fatty acid β-oxidation in brain mitochondria: Insights from high-resolution respirometry in mouse, rat and Drosophila brain, ischemia and aging models. MitoFit Preprints 2023.10. https://doi.org/10.26124/mitofit:2023-0010
-:from the O2k-Network »[[AT Innsbruck Oroboros]]« and »[[LV Riga Liepins E]]«
-|}
 ----
-*'''Dec- 07 Th'''
+* '''May-22 We''' - International Day for Biological Diversity
 :{|
 | width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-|'''"(…) L-Erg [L-Ergothioneine] treatment prevented and delayed cystine lithiasis in a cystinuria mouse model by increasing urinary cystine solubility and restored kidney GSH content and OXPHOS function."''' <br>
+|'''"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."''' <br>
-* Mayayo-Vallverdú C, López de Heredia M, Prat E, González L, Espino Guarch M, Vilches C, Muñoz L, Asensi MA, Serra C, Llebaria A, Casado M, Artuch R, Garrabou G, Garcia-Roves PM, Pallardó FV, Nunes V (2023) The antioxidant l-Ergothioneine prevents cystine lithiasis in the Slc7a9-/- mouse model of cystinuria [[Mayayo-Vallverdu 2023 Redox Biol|»Bioblast link«]]
+* Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. [[Ciccone 2024 J Exp Biol|»Bioblast link«]]
-:from the O2k-Network »[[ES Barcelona Moren C]]« and »[[ES Barcelona Garcia-Roves PM]]«
+:from the O2k-Network »[[NO Tromsoe Wood SH]]«
 |}
 ----
-*'''Dec- 08 Fr'''
+* '''May-23 Th'''
 ----
+* '''May-24 Fr'''
+----
 '''<big>Next Week</big>'''
-Week 50
+Week 22
-*'''Dec- 11 Mo''' - International Mountain Day
+* '''May-27 Mo'''
 :{|
-| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Info: take care]]
-|'''Mountain slopes can be difficult, but O<sub>2</sub> slopes can be smooth with DatLab'''<br>
+|'''Same experimental conditions, but different O<sub>2</sub> fluxes in the left and right chambers?'''<br>
-The default setting of Slope smoothing is 40 data points, corresponding to an interval of 80 s at a data recording interval of 2 s. DatLab 7 expresses it in data points and DatLab 8 in seconds.
+Check for instrumental and operational factors that could be causing O<sub>2</sub> flux differences between the O2k-chambers.
 <br>
-- »'''[https://wiki.oroboros.at/index.php/Flux_/_Slope#Smoothing.2C_noise.2C_and_sample_stability Smoothing in Flux/Slope]'''«<br>
+- »'''[[Different O2 fluxes in left and right chamber]]'''«<br>
 - communicated by »[[Timon-Gomez Alba]]« and »[[Baglivo Eleonora]]«
 |}
 ----
-*'''Dec- 12 Tu'''
+* '''May-28 Tu'''
-:{|
-| width="100" align="center" |[[File:MitoFit Preprints.png|100px|MitoFit pdf]]
-| '''From preprint to Gold Open Access publication in J Biol Chem'''<br>
-* Gnaiger E (2023) Complex II ambiguities ― FADH2 in the electron transfer system. [[Gnaiger 2023 J Biol Chem|»Bioblast link«]]
-|}
 ----
-*'''Dec- 13 We'''
+* '''May-29 We'''
 ----
-*'''Dec- 14 Th'''
+* '''May-30 Th'''
 :{|
-| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|'''"HCR [High capacity runner rats] showed a consistent and robust trend of higher maximal oxygen consumption compared to LCR [low capacity runner rats], especially for long chain FAs [fatty acids]."''' <br>
+|''' "Harmonization of isolation protocols yielding intact and enzymatically active mitochondria are critical to assess mitochondrial respiratory function"'''<br>
-* Fleischman JY, Van den Bergh F, Collins NL, Bowers M, Beard DA, Burant CF (2023) Higher mitochondrial oxidative capacity is the primary molecular differentiator in muscle of rats with high and low intrinsic cardiorespiratory fitness. [[Fleischman 2023 Mol Metab|»Bioblast link«]]
+- »'''[[MiPNet20.06_IsolationMouseHeart-mt|MiPNet20.06 Isolation mouse heart mitochondria]]'''«<br>
-:from the O2k-Network »[[US MI Ann Arbor Beard DA]]«
+- communicated by »[[Cardoso Luiza]]« and »[[Leo Elettra]]«
 |}
 ----
-*'''Dec- 15 Fr'''
+* '''May-31 Fr'''
 ----