IOC61: Difference between revisions

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[[File:O2k-TIP2k.jpg|right|300px|caption]]
<p><img src="/images/thumb/1/14/O2k-TIP2k.jpg/300px-O2k-TIP2k.jpg" _fck_mw_filename="O2k-TIP2k.jpg" _fck_mw_location="right" _fck_mw_width="300" alt="caption" class="fck_mw_right" />
'''O2k-Workshop on High-Resolution Respirometry - Introductory and Advanced'''
<b>O2k-Workshop on High-Resolution Respirometry - Introductory and Advanced</b>
 
</p><p>Schröcken, Vorarlberg, Austria  26 April - 1 May, 2011.  
Schröcken, Vorarlberg, Austria  26 April - 1 May, 2011.  
</p><p>Hotel Körbersee
 
</p><p>For some pictures, see <a href="IOC60 Photos">IOC60</a>
Hotel Körbersee
</p><p>More information on IOC61: http://www.oroboros.at/index.php?nextcourse
 
</p><p><b>Contact</b>: {{{mailaddress|[email protected]}}}<span class="smwttpersist"><span class="smwtticon">warning.png</span><span class="smwttcontent">URIs of the form &quot;{{{mailaddress|[email protected]}}}&quot; are not allowed.</span> </span>
For some pictures, see [[IOC60_Photos|IOC60]]
</p><p><br />
 
</p>
More information on IOC61: http://www.oroboros.at/index.php?nextcourse
<h2>Course structure</h2>
 
<ul><li> Introductory and advanced groups will be working in parallel session, to adjust the programme optimally to the specific needs of the participants in different workpackages.
'''Contact''': [[Has mailaddress::{{{mailaddress|[email protected]}}}]]
</li></ul>
 
<ul><li> Hot Topics in Mitochondrial Physiology: 10+5 min
 
</li></ul>
==Course structure==
<h2>Abstracts</h2>
 
<ul><li><a href=":IOC61 Abstracts">Look at Abstracts &gt;&gt;</a>
* Introductory and advanced groups will be working in parallel session, to adjust the programme optimally to the specific needs of the participants in different workpackages.
</li></ul>
 
<ul><li><a href="http://www.bioblast.at/index.php/Special:AbstractsExtension?event=IOC61">Add your Abstract &gt;&gt;</a>
* Hot Topics in Mitochondrial Physiology: 10+5 min
</li></ul>
 
<p>For the abstract format, see <a _fcknotitle="true" href="Author IOC61">Author IOC61</a>.
==Abstracts==
</p>
 
<h2> Combined introductory and advanced O2k-Workshop with parallel sessions </h2>
*[[:IOC61_Abstracts|Look at Abstracts >>]]
<dl><dd><dl><dd><b>A: Introductory lectures and hands-on experiments for basic HRR in small working teams:</b>
 
</dd></dl>
*[http://www.bioblast.at/index.php/Special:AbstractsExtension?event=IOC61 Add your Abstract >>]
</dd></dl>
 
<p><b>A1. General <a _fcknotitle="true" href="HRR">HRR</a> topics</b>
For the abstract format, see [[Author_IOC61]].
</p>
 
<ul><li> O2k-assembly,  
== Combined introductory and advanced O2k-Workshop with parallel sessions ==
</li><li> <a _fcknotitle="true" href="Polarographic oxygen sensor">Polarographic oxygen sensor</a> (OroboPOS) service,
 
</li><li> POS calibration,
 
</li><li> <a href="DatLab 4.3">DatLab</a> software: principles and operation,
::'''A: Introductory lectures and hands-on experiments for basic HRR in small working teams:'''
</li><li> <a _fcknotitle="true" href="Titration-Injection-microPump">Titration-Injection-microPump</a> - TIP2k.
 
</li></ul>
'''A1. General [[HRR]] topics'''
<p><b>A2. Protocols in HRR</b>
* O2k-assembly,
</p>
* [[Polarographic oxygen sensor]] (OroboPOS) service,
<ul><li> Introduction to fundamental elements of mitochondrial respiratory physiology,
* POS calibration,
</li><li> Design and examples of experimental protocols with intact cells, permeabilized cells and tissues, isolated mitochondria.
* [[DatLab 4.3|DatLab]] software: principles and operation,
</li></ul>
* [[Titration-Injection-microPump]] - TIP2k.
<p><b>A3. Hands-on experiments with the O2k</b>
'''A2. Protocols in HRR'''
</p>
* Introduction to fundamental elements of mitochondrial respiratory physiology,
<ul><li> Measurement of <a _fcknotitle="true" href="Instrumental background oxygen flux">Instrumental background oxygen flux</a>, manual and TIP2k-feedback control,
* Design and examples of experimental protocols with intact cells, permeabilized cells and tissues, isolated mitochondria.
</li><li> Intact cells: Respirometric reference assay with yeast, <a _fcknotitle="true" href="Phosphorylation control protocol">Phosphorylation control protocol</a>,
'''A3. Hands-on experiments with the O2k'''
</li><li> Preparation of <a _fcknotitle="true" href="Permeabilized muscle fibre">Permeabilized muscle fibre</a>s,
* Measurement of [[instrumental background oxygen flux]], manual and TIP2k-feedback control,
</li><li> HRR with permeabilized muscle fibres.
* Intact cells: Respirometric reference assay with yeast, [[phosphorylation control protocol]],
</li></ul>
* Preparation of [[permeabilized muscle fibre]]s,
<p><b>A4. Presentation and interpretation of <a _fcknotitle="true" href="Oxygen flux">Oxygen flux</a></b>
* HRR with permeabilized muscle fibres.
</p>
'''A4. Presentation and interpretation of [[oxygen flux]]'''
<ul><li> Normalization of respiration per tissue or per <a _fcknotitle="true" href="Mitochondrial marker">Mitochondrial marker</a>,
* Normalization of respiration per tissue or per [[mitochondrial marker]],
</li><li> Coupling control ratios,
* Coupling control ratios,
</li><li> Substrate control ratios,
* Substrate control ratios,
</li><li> Generalized flux control ratios.  
* Generalized flux control ratios.
</li></ul>
'''A5. Overview on O2k-MultiSensor applications - Simultaneous measurement of respiration and:'''
<p><b>A5. Overview on O2k-MultiSensor applications - Simultaneous measurement of respiration and:</b>
* Mitochondrial membrane potential (TPP+ electrode),
</p>
* Acidification (pH electrode),
<ul><li> Mitochondrial membrane potential (TPP+ electrode),
* Nitric oxide (amperometric),
</li><li> Acidification (pH electrode),
* Spectrophotometry (cytochrome spectra),
</li><li> Nitric oxide (amperometric),
* Spectrofluorimetry (Amplex red, safranin, etc.)
</li><li> Spectrophotometry (cytochrome spectra),
 
</li><li> Spectrofluorimetry (Amplex red, safranin, etc.)
::'''B: Advanced users will join in small working teams in parallel sessions, with specific topics:
</li></ul>
'''
<dl><dd><dl><dd><b>B: Advanced users will join in small working teams in parallel sessions, with specific topics:</b>
'''B1. O2k-MultiSensor and TIP2k'''
</dd></dl>
* Hands-on MultiSensor TPP<sup>+</sup> applications, see [[TPP special interest group during an International Oxygraph Course]]
</dd></dl>
* Oxygen kinetics: experimental examples with TIP2k-feedback control (oxystat) and data analysis,
<p>
* ADP kinetics: experimental examples with TIP2-steady state control and data analysis,
<b>B1. O2k-MultiSensor and TIP2k</b>
* pH-stat feedback control,
</p>
'''B2. Theoretical perspectives'''
<ul><li> Hands-on MultiSensor TPP<sup>+</sup> applications, see <a _fcknotitle="true" href="TPP special interest group during an International Oxygraph Course">TPP special interest group during an International Oxygraph Course</a>
* Advanced experimental protocols,  
</li><li> Oxygen kinetics: experimental examples with TIP2k-feedback control (oxystat) and data analysis,  
* Quality control - towards [[quality assurance]];
</li><li> ADP kinetics: experimental examples with TIP2-steady state control and data analysis,  
 
</li><li> pH-stat feedback control,
* ''Add your topic'':
</li></ul>
 
<p><b>B2. Theoretical perspectives</b>
==Participants and areas of interest==
</p>
 
<ul><li> Advanced experimental protocols,  
* [[Alexeyev M|'''Alexeyev Mikhail, USA''']]: Mouse modeling of mitochondrial disorders caused by mtDNA mutations.
</li><li> Quality control - towards <a _fcknotitle="true" href="Quality assurance">Quality assurance</a>;
* [[Angelin A|'''Angelin Alessia, USA''']]: Function in muscle tissue; in particular human muscle disorder (muscular dystrophy).
</li></ul>
* [[Balboa Castillo E|'''Balboa Castillo Elisa, Chile''']]: Increase in mitochondrial cholesterol and dysfunction in NPC.
<ul><li> <i>Add your topic</i>:
* [[Castelein N|'''Castelein Natascha, Belgium''']]: The role of mitochondria in dietary restriction induced lifespan extension in ''C. elegan''s.
</li></ul>
* [[Gabrielova E|'''Gabrielova Eva, Czech Republic''']]: [[Gabrielova IOC61|Evaluation of oxygen consumption in rat heart mitochondria treatment by polyphenol compounds.]]
<h2>Participants and areas of interest</h2>
* [[Hals I|'''Hals Ingrid, Norway''']]: Effects of hypoxia in pancreatic beta-cells on mitochondrial functions.
<ul><li> <a href="Alexeyev M"><b>Alexeyev Mikhail, USA</b></a>: Mouse modeling of mitochondrial disorders caused by mtDNA mutations.
* [[Hancock S|'''Hancock Saege, USA''']]
</li><li> <a href="Angelin A"><b>Angelin Alessia, USA</b></a>: Function in muscle tissue; in particular human muscle disorder (muscular dystrophy).
* [[Kasal D|'''Kasal Daniel, Brazil''']]: Modulation of cardiomyocyte respiration by bone marrow stem cells.
</li><li> <a href="Balboa Castillo E"><b>Balboa Castillo Elisa, Chile</b></a>: Increase in mitochondrial cholesterol and dysfunction in NPC.
* [[Labieniec M|'''Labieniec Magdalena, Poland''']]: Mitochondrial respiratory capacity; mt membrane potential; calcium homeostasis; mitochondria in health and pathology (diabetes, neurodegenerative diseases, cancer); mt function; ROS
</li><li> <a href="Castelein N"><b>Castelein Natascha, Belgium</b></a>: The role of mitochondria in dietary restriction induced lifespan extension in <i>C. elegan</i>s.
* [[Le C|'''Le Catherine, USA''']]: Implications of mitochondrial dysfunction in heart disease; cardiomypathy; ROS; mitochondrial function, respiration, membrane potential.
</li><li> <a href="Gabrielova E"><b>Gabrielova Eva, Czech Republic</b></a>: <a href="Gabrielova IOC61">Evaluation of oxygen consumption in rat heart mitochondria treatment by polyphenol compounds.</a>
* [[Magnifico MC|'''Magnifico Maria Chiara, Italy''']]: Measurement of oxygen consumption, respiratory flux and membrane potential in HaCaT cells.
</li><li> <a href="Hals I"><b>Hals Ingrid, Norway</b></a>: Effects of hypoxia in pancreatic beta-cells on mitochondrial functions.
* [[Mandl M|'''Mandl Markus, Vienna''']]
</li><li> <a href="Hancock S"><b>Hancock Saege, USA</b></a>
* [[Manjeri G|'''Manjeri Ganesh, The Netherlands''']]: Understanding oxygen flux in mitochondrial disorders working with skin fibroblasts.
</li><li> <a href="Kasal D"><b>Kasal Daniel, Brazil</b></a>: Modulation of cardiomyocyte respiration by bone marrow stem cells.
* [[Marelsson S|'''Marelsson Sigurdur, Sweden''']]: The role of mitochondrial dysfunction in encephalopathy.
</li><li> <a href="Labieniec M"><b>Labieniec Magdalena, Poland</b></a>: Mitochondrial respiratory capacity; mt membrane potential; calcium homeostasis; mitochondria in health and pathology (diabetes, neurodegenerative diseases, cancer); mt function; ROS
* [[Morein T|'''Morein Torbjörn, Sweden''']]
</li><li> <a href="Le C"><b>Le Catherine, USA</b></a>: Implications of mitochondrial dysfunction in heart disease; cardiomypathy; ROS; mitochondrial function, respiration, membrane potential.
* [[Nuskova H|'''Nuskova Hana, Czech Republic''']]: [[Nuskova IOC61|Mitochondrial Energetics in Health and Disease]].
</li><li> <a href="Magnifico MC"><b>Magnifico Maria Chiara, Italy</b></a>: Measurement of oxygen consumption, respiratory flux and membrane potential in HaCaT cells.
* [[Sanz A|'''Sanz Alberto, Finland''']]: Mechanism causing aging and the role of mitochondria in this process.
</li><li> <a href="Mandl M"><b>Mandl Markus, Vienna</b></a>
* [[Scherer Alves L|'''Scherer Alves Leonardo, USA''']]: Mitochondrial Physiology as a force of human adaptation and evolution to different geographic niches and the relation between the environment and mitochondrial genes in human common diseases.
</li><li> <a href="Manjeri G"><b>Manjeri Ganesh, The Netherlands</b></a>: Understanding oxygen flux in mitochondrial disorders working with skin fibroblasts.
* [[Watala C|'''Watala Cezary, Poland''']]: Mitocondrial respiratory capacity; mt membrane potential; calcium homeostasis; mitochondria in health and pathology (diabetes, neurodegenerative diseases, cancer); mt function; ROS
</li><li> <a href="Marelsson S"><b>Marelsson Sigurdur, Sweden</b></a>: The role of mitochondrial dysfunction in encephalopathy.
* [[Yokota T|'''Yokota Takashi, Denmark''']]: The role of mitochondria in chronic heart failure, diabetes, or obesity.
</li><li> <a href="Morein T"><b>Morein Torbjörn, Sweden</b></a>
* [[Zurmanova J|'''Zurmanova Jitka, Czech Republic''']]: Mechanisms of regulation of OXPHOS by local ADP in muscle cells.
</li><li> <a href="Nuskova H"><b>Nuskova Hana, Czech Republic</b></a>: <a href="Nuskova IOC61">Mitochondrial Energetics in Health and Disease</a>.
 
</li><li> <a href="Sanz A"><b>Sanz Alberto, Finland</b></a>: Mechanism causing aging and the role of mitochondria in this process.
== Tutors ==
</li><li> <a href="Scherer Alves L"><b>Scherer Alves Leonardo, USA</b></a>: Mitochondrial Physiology as a force of human adaptation and evolution to different geographic niches and the relation between the environment and mitochondrial genes in human common diseases.
 
</li><li> <a href="Watala C"><b>Watala Cezary, Poland</b></a>: Mitocondrial respiratory capacity; mt membrane potential; calcium homeostasis; mitochondria in health and pathology (diabetes, neurodegenerative diseases, cancer); mt function; ROS
* [[Gnaiger E|'''Erich Gnaiger''']] (Innsbruck Medical University, AT; OROBOROS INSTRUMENTS, AT)
</li><li> <a href="Yokota T"><b>Yokota Takashi, Denmark</b></a>: The role of mitochondria in chronic heart failure, diabetes, or obesity.
* [[Fasching M|'''Mario Fasching''']] (OROBOROS INSTRUMENTS, AT)
</li><li> <a href="Zurmanova J"><b>Zurmanova Jitka, Czech Republic</b></a>: Mechanisms of regulation of OXPHOS by local ADP in muscle cells.
* [[Pesta D|'''Dominik Pesta''']] (Innsbruck Medical University, AT)
</li></ul>
* [[Sumbalova Z|'''Zuzana Sumbalova''']] (OROBOROS INSTRUMENTS, AT)
<h2> Tutors </h2>
 
<ul><li> <a href="Gnaiger E"><b>Erich Gnaiger</b></a> (Innsbruck Medical University, AT; OROBOROS INSTRUMENTS, AT)
==Discussion==
</li><li> <a href="Fasching M"><b>Mario Fasching</b></a> (OROBOROS INSTRUMENTS, AT)
 
</li><li> <a href="Pesta D"><b>Dominik Pesta</b></a> (Innsbruck Medical University, AT)
[[Talk:IOC61|Continue the Discussion >>]]
</li><li> <a href="Sumbalova Z"><b>Zuzana Sumbalova</b></a> (OROBOROS INSTRUMENTS, AT)
 
</li></ul>
[[Category:IOC61]]
<h2>Discussion</h2>
[[Category:OroboroPedia]]
<p><a href="Talk:IOC61">Continue the Discussion &gt;&gt;</a>
[[Category:Events]]
</p><a _fcknotitle="true" href="Category:IOC61">Category:IOC61</a> <a _fcknotitle="true" href="Category:OroboroPedia">OroboroPedia</a> <a _fcknotitle="true" href="Category:Events">Events</a>

Revision as of 12:32, 5 April 2011

<img src="/images/thumb/1/14/O2k-TIP2k.jpg/300px-O2k-TIP2k.jpg" _fck_mw_filename="O2k-TIP2k.jpg" _fck_mw_location="right" _fck_mw_width="300" alt="caption" class="fck_mw_right" /> O2k-Workshop on High-Resolution Respirometry - Introductory and Advanced

Schröcken, Vorarlberg, Austria 26 April - 1 May, 2011.

Hotel Körbersee

For some pictures, see <a href="IOC60 Photos">IOC60</a>

More information on IOC61: http://www.oroboros.at/index.php?nextcourse

Contact: [email protected]warning.pngURIs of the form "[email protected]" are not allowed.


Course structure

  • Introductory and advanced groups will be working in parallel session, to adjust the programme optimally to the specific needs of the participants in different workpackages.
  • Hot Topics in Mitochondrial Physiology: 10+5 min

Abstracts

  • <a href=":IOC61 Abstracts">Look at Abstracts >></a>

For the abstract format, see <a _fcknotitle="true" href="Author IOC61">Author IOC61</a>.

Combined introductory and advanced O2k-Workshop with parallel sessions

A: Introductory lectures and hands-on experiments for basic HRR in small working teams:

A1. General <a _fcknotitle="true" href="HRR">HRR</a> topics

  • O2k-assembly,
  • <a _fcknotitle="true" href="Polarographic oxygen sensor">Polarographic oxygen sensor</a> (OroboPOS) service,
  • POS calibration,
  • <a href="DatLab 4.3">DatLab</a> software: principles and operation,
  • <a _fcknotitle="true" href="Titration-Injection-microPump">Titration-Injection-microPump</a> - TIP2k.

A2. Protocols in HRR

  • Introduction to fundamental elements of mitochondrial respiratory physiology,
  • Design and examples of experimental protocols with intact cells, permeabilized cells and tissues, isolated mitochondria.

A3. Hands-on experiments with the O2k

  • Measurement of <a _fcknotitle="true" href="Instrumental background oxygen flux">Instrumental background oxygen flux</a>, manual and TIP2k-feedback control,
  • Intact cells: Respirometric reference assay with yeast, <a _fcknotitle="true" href="Phosphorylation control protocol">Phosphorylation control protocol</a>,
  • Preparation of <a _fcknotitle="true" href="Permeabilized muscle fibre">Permeabilized muscle fibre</a>s,
  • HRR with permeabilized muscle fibres.

A4. Presentation and interpretation of <a _fcknotitle="true" href="Oxygen flux">Oxygen flux</a>

  • Normalization of respiration per tissue or per <a _fcknotitle="true" href="Mitochondrial marker">Mitochondrial marker</a>,
  • Coupling control ratios,
  • Substrate control ratios,
  • Generalized flux control ratios.

A5. Overview on O2k-MultiSensor applications - Simultaneous measurement of respiration and:

  • Mitochondrial membrane potential (TPP+ electrode),
  • Acidification (pH electrode),
  • Nitric oxide (amperometric),
  • Spectrophotometry (cytochrome spectra),
  • Spectrofluorimetry (Amplex red, safranin, etc.)
B: Advanced users will join in small working teams in parallel sessions, with specific topics:

B1. O2k-MultiSensor and TIP2k

  • Hands-on MultiSensor TPP+ applications, see <a _fcknotitle="true" href="TPP special interest group during an International Oxygraph Course">TPP special interest group during an International Oxygraph Course</a>
  • Oxygen kinetics: experimental examples with TIP2k-feedback control (oxystat) and data analysis,
  • ADP kinetics: experimental examples with TIP2-steady state control and data analysis,
  • pH-stat feedback control,

B2. Theoretical perspectives

  • Advanced experimental protocols,
  • Quality control - towards <a _fcknotitle="true" href="Quality assurance">Quality assurance</a>;
  • Add your topic:

Participants and areas of interest

  • <a href="Alexeyev M">Alexeyev Mikhail, USA</a>: Mouse modeling of mitochondrial disorders caused by mtDNA mutations.
  • <a href="Angelin A">Angelin Alessia, USA</a>: Function in muscle tissue; in particular human muscle disorder (muscular dystrophy).
  • <a href="Balboa Castillo E">Balboa Castillo Elisa, Chile</a>: Increase in mitochondrial cholesterol and dysfunction in NPC.
  • <a href="Castelein N">Castelein Natascha, Belgium</a>: The role of mitochondria in dietary restriction induced lifespan extension in C. elegans.
  • <a href="Gabrielova E">Gabrielova Eva, Czech Republic</a>: <a href="Gabrielova IOC61">Evaluation of oxygen consumption in rat heart mitochondria treatment by polyphenol compounds.</a>
  • <a href="Hals I">Hals Ingrid, Norway</a>: Effects of hypoxia in pancreatic beta-cells on mitochondrial functions.
  • <a href="Hancock S">Hancock Saege, USA</a>
  • <a href="Kasal D">Kasal Daniel, Brazil</a>: Modulation of cardiomyocyte respiration by bone marrow stem cells.
  • <a href="Labieniec M">Labieniec Magdalena, Poland</a>: Mitochondrial respiratory capacity; mt membrane potential; calcium homeostasis; mitochondria in health and pathology (diabetes, neurodegenerative diseases, cancer); mt function; ROS
  • <a href="Le C">Le Catherine, USA</a>: Implications of mitochondrial dysfunction in heart disease; cardiomypathy; ROS; mitochondrial function, respiration, membrane potential.
  • <a href="Magnifico MC">Magnifico Maria Chiara, Italy</a>: Measurement of oxygen consumption, respiratory flux and membrane potential in HaCaT cells.
  • <a href="Mandl M">Mandl Markus, Vienna</a>
  • <a href="Manjeri G">Manjeri Ganesh, The Netherlands</a>: Understanding oxygen flux in mitochondrial disorders working with skin fibroblasts.
  • <a href="Marelsson S">Marelsson Sigurdur, Sweden</a>: The role of mitochondrial dysfunction in encephalopathy.
  • <a href="Morein T">Morein Torbjörn, Sweden</a>
  • <a href="Nuskova H">Nuskova Hana, Czech Republic</a>: <a href="Nuskova IOC61">Mitochondrial Energetics in Health and Disease</a>.
  • <a href="Sanz A">Sanz Alberto, Finland</a>: Mechanism causing aging and the role of mitochondria in this process.
  • <a href="Scherer Alves L">Scherer Alves Leonardo, USA</a>: Mitochondrial Physiology as a force of human adaptation and evolution to different geographic niches and the relation between the environment and mitochondrial genes in human common diseases.
  • <a href="Watala C">Watala Cezary, Poland</a>: Mitocondrial respiratory capacity; mt membrane potential; calcium homeostasis; mitochondria in health and pathology (diabetes, neurodegenerative diseases, cancer); mt function; ROS
  • <a href="Yokota T">Yokota Takashi, Denmark</a>: The role of mitochondria in chronic heart failure, diabetes, or obesity.
  • <a href="Zurmanova J">Zurmanova Jitka, Czech Republic</a>: Mechanisms of regulation of OXPHOS by local ADP in muscle cells.

Tutors

  • <a href="Gnaiger E">Erich Gnaiger</a> (Innsbruck Medical University, AT; OROBOROS INSTRUMENTS, AT)
  • <a href="Fasching M">Mario Fasching</a> (OROBOROS INSTRUMENTS, AT)
  • <a href="Pesta D">Dominik Pesta</a> (Innsbruck Medical University, AT)
  • <a href="Sumbalova Z">Zuzana Sumbalova</a> (OROBOROS INSTRUMENTS, AT)

Discussion

<a href="Talk:IOC61">Continue the Discussion >></a>

<a _fcknotitle="true" href="Category:IOC61">Category:IOC61</a> <a _fcknotitle="true" href="Category:OroboroPedia">OroboroPedia</a> <a _fcknotitle="true" href="Category:Events">Events</a>

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