Injarabian 2020 Sci Rep: Difference between revisions
No edit summary |
No edit summary ย |
||
Line 1: | Line 1: | ||
{{Publication | {{Publication | ||
|title=Injarabian L, Scherlinger M, Devin A, Ransac S, Lykkesfeldt J, Marteyn BS (2020) Ascorbate maintains a low plasma oxygen level . Sci Rep 10:10659. | |title=Injarabian L, Scherlinger M, Devin A, Ransac S, Lykkesfeldt J, Marteyn BS (2020) Ascorbate maintains a low plasma oxygen level. Sci Rep 10:10659. | ||
|info=[https://www.ncbi.nlm.nih.gov/pubmed/32606354 PMID: 32606354 Open Access] | |info=[https://www.ncbi.nlm.nih.gov/pubmed/32606354 PMID: 32606354 Open Access] | ||
|authors=Injarabian | |authors=Injarabian Louise, Scherlinger Marc, Devin Anne, Ransac Stephane, Lykkesfeldt Jens, Marteyn Benoit S | ||
|year=2020 | |year=2020 | ||
|journal=Sci Rep | |journal=Sci Rep |
Latest revision as of 20:43, 8 July 2020
Injarabian L, Scherlinger M, Devin A, Ransac S, Lykkesfeldt J, Marteyn BS (2020) Ascorbate maintains a low plasma oxygen level. Sci Rep 10:10659. |
Injarabian Louise, Scherlinger Marc, Devin Anne, Ransac Stephane, Lykkesfeldt Jens, Marteyn Benoit S (2020) Sci Rep
Abstract: In human blood, oxygen is mainly transported by red blood cells. Accordingly, the dissolved oxygen level in plasma is expected to be limited, although it has not been quantified yet. Here, by developing dedicated methods and tools, we determined that human plasma pO2 = 8.4 mmHg (1.1% O2). Oxygen solubility in plasma was believed to be similar to water. Here we reveal that plasma has an additional ascorbate-dependent oxygen-reduction activity. Plasma experimental oxygenation oxidizes ascorbate (49.5 ฮผM in fresh plasma vs < 2 ฮผM in oxidized plasma) and abolishes this capacity, which is restored by ascorbate supplementation. We confirmed these results in vivo, showing that the plasma pO2 is significantly higher in ascorbate-deficient guinea pigs (Ascorbateplasma < 2 ฮผM), compared to control (Ascorbateplasma > 15 ฮผM). Plasma low oxygen level preserves the integrity of oxidation-sensitive components such as ubiquinol. Circulating leucocytes are well adapted to these conditions, since the abundance of their mitochondrial network is limited. These results shed a new light on the importance of oxygen exposure on leucocyte biological study, in regards with the reducing conditions they encounter in vivo; but also, on the manipulation of blood products to improve their integrity and potentially improve transfusions' efficacy.
โข Bioblast editor: Plangger M โข O2k-Network Lab: FR Bordeaux Devin A
Labels: MiParea: Respiration, Instruments;methods
Organism: Human
Tissue;cell: Blood cells
Preparation: Oxidase;biochemical oxidation
HRR: Oxygraph-2k
2020-07