MiPNet06.06 Chemical O2 background
| Kuznetsov AV, Gnaiger E. Oxygraph assay of cytochrome c oxidase activity: Chemical background correction. Mitochondr. Physiol. Network 06.06. |
» [[Has info::MiPNet06.06]]
) Mitochondr. Physiol. Network
Abstract: Oxygraphic determination of cytochrome c oxidase activity in the presence of TMPD, ascorbate and cytochrome c requires consideration of chemical background oxygen consumption. Several compounds are readily oxidized by molecular oxygen when dissolved in water. This leads to a significant chemical oxygen consumption in the absence of any respiring biological sample. The rate of this autoxidation represents a chemical background which strongly depends on experimental conditions, such as temperature, chemical composition, pH and oxygen concentration. Moreover, the rate of autoxidation may be significantly catalyzed by metal traces and metal-containing proteins (e.g. cytochrome c). Therefore, high-resolution respirometric measurement of oxygen flux ultimately depends on chemical background correction analyzed under experimental conditions over the entire oxygen range.
• O2k-Network Lab: AT_Innsbruck_OROBOROS
Labels:
Preparation: Oxidase; Biochemical Oxidation, Enzyme
Regulation: Respiration; OXPHOS; ETS Capacity
HRR: Oxygraph-2k, Protocol, Theory, MiPNet-Publication
O2k-SOP
