Brewer 2004 Mitochondrion
Brewer GJ, Jones TT, Wallimann T, Schlattner U (2004) Higher respiratory rates and improved creatine stimulation in brain mitochondria isolated with anti-oxidants. Mitochondrion 4: 49-57. |
Β» [[Has info::PMID: 16120374]]
Brewer GJ, Jones TT, Wallimann T, Schlattner U (2004) Mitochondrion
Abstract: We tested the effect of an anti-oxidant mixture on respiration in isolated rat brain mitochondria. Mitochondria were isolated in mannitol/sucrose/EGTA/BSAΒ±SCAVEGRβ’ anti-oxidants (SOD, catalase, vitamin E, vitamin E acetate, and glutathione reduced). TBARS were reduced by greater than 40% with SCAVEGR. Respiration driven by ADP showed a two-fold higher Vmax and a 15% higher respiratory control ratio when mitochondria were prepared with SCAVEGR. SCAVEGR also stabilized the octameric state of mitochondrial creatine kinase and thus improved creatine-stimulated respiration. These results suggest that significant improvements in brain mitochondrial function are obtained by isolation in the presence of an anti-oxidants mixture. β’ Keywords: Anti-oxidant, Brain mitochondria, Creatine kinase, Respiration, ROS
Labels:
Organism: Rat
Tissue;cell: Neurons; Brain
Preparation: Isolated Mitochondria
Regulation: Respiration; OXPHOS; ETS Capacity
HRR: Oxygraph-2k